Date published: 2025-9-7

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RhoGEF p115 抗体 (D-11): sc-166341

5.0(1)
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说明书
  • RhoGEF p115抗体(D-11)是小鼠单克隆IgG2a κ, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的RhoGEF p115的N-terminus附近的氨基酸48-75之间
  • 推荐用于 human 来源的RhoGEF p115 WB, IP, IF 和 ELISA检测
  • 目前,我们还没有完成RhoGEF p115 抗体 (D-11)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

GTP酶的Ras超家族可以细分为Ras、Rho/Rac、Sar、Rab、Arf和Ran等亚家族,并控制细胞功能的多个方面,包括细胞骨架重排、核信号传导和细胞生长。GTP酶的Ras超家族作为受调控的开关,在生物活性GTP结合形式和非活性GDP结合形式之间切换。这种激活是由鸟嘌呤核苷酸交换因子(GEFs)催化的。与Dbl相关的蛋白质是一大类结构相关的分子,它们具有为Ras家族的特定成员催化GEF活性的共同能力。与Dbl相关的蛋白质包括FGD1、RhoGEF p115、Lsc、Lfc、Lbc和Brx。RhoGEF p115、Lsc、Lbc和Lfc具有序列同源性,并显示出对Rho家族GTP酶的交换活性。RhoGEF p115(Lsc的人类同源物)可催化Rho的GEF活性,但不催化Rac、Cdc42或Ras GTP酶的GEF活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RhoGEF p115 抗体 (D-11) 参考文献:

  1. p115-RhoGEF的N端富含酸性的区域发生突变,使α13的结合与α13促进的质膜招募分离。  |  Bhattacharyya, R. and Wedegaertner, PB. 2003. FEBS Lett. 540: 211-6. PMID: 12681510
  2. 协调golgin系链和SNARE组装:GM130 以 p115 调节的方式与突触融合蛋白 5 结合。  |  Diao, A., et al. 2008. J Biol Chem. 283: 6957-67. PMID: 18167358
  3. G(12/13)偶联受体可导致p115-RhoGEF的可逆易位。  |  Meyer, BH., et al. 2008. J Cell Biochem. 104: 1660-70. PMID: 18320579
  4. Galpha12和Galpha13介导的p115-RhoGEF在质膜上的聚集存在差异。  |  Bhattacharyya, R., et al. 2009. Cell Signal. 21: 996-1006. PMID: 19249348
  5. P115 RhoGEF和微管决定凋亡细胞从上皮中排出的方向。  |  Slattum, G., et al. 2009. J Cell Biol. 186: 693-702. PMID: 19720875
  6. GEF开关的调节:p115-RhoGEF的内在鸟苷酸交换活性的自我抑制。  |  Chen, Z., et al. 2011. Protein Sci. 20: 107-17. PMID: 21064165
  7. PRL-1 蛋白通过与 p115 Rho GTPase-activating 蛋白的 Src 同源 3 结构域的非经典相互作用,促进 ERK1/2 和 RhoA 蛋白的活化。  |  Bai, Y., et al. 2011. J Biol Chem. 286: 42316-42324. PMID: 22009749
  8. p115-RhoGEF的激活需要Gα13和Dbl同源结构域的直接结合。  |  Chen, Z., et al. 2012. J Biol Chem. 287: 25490-500. PMID: 22661716
  9. JAM-A 上的张力通过 GEF-H1 和 p115 RhoGEF 激活 RhoA。  |  Scott, DW., et al. 2016. Mol Biol Cell. 27: 1420-30. PMID: 26985018
  10. p115 RhoGEF,一种GTP酶激活蛋白,用于激活Galpha12和Galpha13。  |  Kozasa, T., et al. 1998. Science. 280: 2109-11. PMID: 9641915

订购信息

产品名称产品编号规格价格数量收藏夹

RhoGEF p115 抗体 (D-11)

sc-166341
200 µg/ml
RMB2377.00

RhoGEF p115 (D-11) 中和勝肽

sc-166341 P
100 µg/0.5 ml
RMB511.00

Will your p115RhoGef antibodies pick up other species such as rat and mouse

Asked by: RomanG
Thank you for your question. We do offer one antibody currently, RhoGEF p115/Lsc Antibody (B-7): sc-373720, which is recommended for detection of the mouse and rat homolog protein of RhoGEF p115, known as Lsc.
Answered by: Technical Support
Date published: 2020-04-22

What application is the blocking peptide sc-166341 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Good Western blot data of RhoGEF p115 expressionGood Western blot data of RhoGEF p115 expression in non-transfected and human RhoGEF p115 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-09-15
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RhoGEF p115 抗体 (D-11) is rated 5.0 out of 5 by 1.
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