Date published: 2025-9-7

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Rho GDIα 抗体 (G-2): sc-373724

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说明书
  • Rho GDI alpha 抗体 G-2 是小鼠单克隆 IgG2b κ,Rho GDIα抗体, 在19篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Rho GDIα的N-terminus的氨基酸2-29之间
  • Rho GDI alpha 抗体 (G-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ,检测 mouse, rat, human 和 来源的Rho GDIα; 也和以下物种反应,包括: 和 equine, canine, bovine, porcine and avian
  • 抗Rho GDI alpha抗体(G-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rho GDIα (G-2): sc-373724免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Rho GDIα Antibody (G-2) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与Rho GDIα Antibody (G-2) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

Rho GDIα抗体(G-2)是一种IgG2b κ小鼠单克隆Rho GDI alpha抗体(也称为ARHGDIA抗体、GDIA1抗体或Rho GDI 1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的Rho GDI alpha蛋白。Rho GDIα抗体(G-2)既可以是非偶联的抗Rho GDI alpha抗体形式,也可以是多种偶联形式的抗Rho GDI alpha抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ras超家族的小GTP结合蛋白成员是多种细胞信号通路的关键介质,包括导致细胞增殖、细胞骨架组织和分泌的通路。这些蛋白的活性GTP结合形式向其非活性GDP结合形式的反向转化受到两类调节蛋白的影响:一类是改变GTP结合蛋白的内在GTP酶活性,另一类是改变GDP/GTP交换速率。鸟苷酸释放因子(GRFs)增加GDP解离速率,而GDP解离抑制因子(GDIs)降低解离速率。Rho GDIα,也称为ARHGDIA或GDIA1,是包含204个氨基酸的Rho GDI家族蛋白成员。Rho GDIα定位于细胞质中,抑制GDP从Rho蛋白上解离,从而阻止GTP与Rho蛋白结合并随后激活Rho蛋白。在人类中,Rho GDIα可以在Ser 101位点被p21活化激酶(αPAK)磷酸化,这一事件抑制了Rho GDIα的活性,并可能导致对某些Rho GDIα靶向蛋白的正反馈调节。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rho GDIα 抗体 (G-2) 参考文献:

  1. 缺乏Rho GDIα的小鼠的肾脏和生殖器官逐渐受损。  |  Togawa, A., et al. 1999. Oncogene. 18: 5373-80. PMID: 10498891
  2. Rac/Rho-GDI 复合物激活 NADPH 氧化酶的机制。  |  Di-Poï, N., et al. 2001. Biochemistry. 40: 10014-22. PMID: 11513579
  3. Pak1 对 RhoGDI 的磷酸化介导了 Rac GTPase 的解离。  |  DerMardirossian, C., et al. 2004. Mol Cell. 15: 117-27. PMID: 15225553
  4. rho GDP解离抑制剂的抑制和穿梭功能解耦。  |  Dransart, E., et al. 2005. J Biol Chem. 280: 4674-83. PMID: 15513926
  5. Src 对 RhoGDI 的磷酸化调节 Rho GTPase 的结合和细胞膜-膜循环。  |  DerMardirossian, C., et al. 2006. Mol Biol Cell. 17: 4760-8. PMID: 16943322
  6. Rho GDP解离抑制剂α与雌激素受体α相互作用,影响雌激素的反应性。  |  El Marzouk, S., et al. 2007. J Mol Endocrinol. 39: 249-59. PMID: 17909265
  7. Bcr GTPase 激活结构域的活性是通过与 Rho 鸟嘌呤核苷酸解离抑制剂的直接蛋白质/蛋白质相互作用来调节的。  |  Kweon, SM., et al. 2008. J Biol Chem. 283: 3023-3030. PMID: 18070886
  8. 通过 RhoGDIα 的 cAMP 依赖性磷酸化调节 RhoA 信号传导。  |  Oishi, A., et al. 2012. J Biol Chem. 287: 38705-15. PMID: 23012358
  9. 对赖氨酸乙酰化调节Rho基本调节因子RhoGDIα的结构和机理的深入理解。  |  Kuhlmann, N., et al. 2016. J Biol Chem. 291: 5484-5499. PMID: 26719334
  10. RhoGDIα抑制胶质瘤干细胞的自我更新和肿瘤形成。  |  Wu, F., et al. 2016. Oncotarget. 7: 61619-61629. PMID: 27557508
  11. 鉴定两种人类 Rho GDP 解离阻抑剂蛋白,它们的过表达会导致肌动蛋白细胞骨架的破坏。  |  Leffers, H., et al. 1993. Exp Cell Res. 209: 165-74. PMID: 8262133
  12. 远端 17q 的体细胞杂交面板:GDIA1 映射到 17q25.3。  |  Wagner, T., et al. 1997. Cytogenet Cell Genet. 76: 172-5. PMID: 9186513

订购信息

产品名称产品编号规格价格数量收藏夹

Rho GDIα 抗体 (G-2)

sc-373724
200 µg/ml
RMB2377.00

Rho GDIα (G-2): m-IgGκ BP-HRP 套装

sc-522470
200 µg Ab, 40 µg BP
RMB2662.00

Rho GDIα 抗体 (G-2) AC

sc-373724 AC
500 µg/ml, 25% agarose
RMB3129.00

Rho GDIα 抗体 (G-2) HRP

sc-373724 HRP
200 µg/ml
RMB2377.00

Rho GDIα 抗体 (G-2) FITC

sc-373724 FITC
200 µg/ml
RMB2482.00

Rho GDIα 抗体 (G-2) PE

sc-373724 PE
200 µg/ml
RMB2580.00

Rho GDIα 抗体 (G-2) Alexa Fluor® 488

sc-373724 AF488
200 µg/ml
RMB2685.00

Rho GDIα 抗体 (G-2) Alexa Fluor® 546

sc-373724 AF546
200 µg/ml
RMB2685.00

Rho GDIα 抗体 (G-2) Alexa Fluor® 594

sc-373724 AF594
200 µg/ml
RMB2685.00

Rho GDIα 抗体 (G-2) Alexa Fluor® 647

sc-373724 AF647
200 µg/ml
RMB2685.00

Rho GDIα 抗体 (G-2) Alexa Fluor® 680

sc-373724 AF680
200 µg/ml
RMB2685.00

Rho GDIα 抗体 (G-2) Alexa Fluor® 790

sc-373724 AF790
200 µg/ml
RMB2685.00

Rho GDIα (G-2) 中和勝肽

sc-373724 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-373724 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent in WBDetects very strong single band of the appropriate size in cell lines and tissue lysates of mouse and human origin. Can easily use at 1:4,000 or 1:8,000 (maybe lot dependent though).
Date published: 2018-05-17
Rated 5 out of 5 by from Great antibody!!This antibody is really good and spesific for Rho-GDIα expression in HEK293T whole cell lysates.
Date published: 2018-03-17
Rated 5 out of 5 by from Work perfectlyUse at 1:400 on human primary macrophages and work perfectly without any other bands or background
Date published: 2017-10-17
Rated 4 out of 5 by from it works well on IHC and western blotit works well it works well on IHC and western blot for human skin tissue
Date published: 2017-10-12
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of Rho GDIα expression in K-562, MCF7, Jurkat, PC-12, HL-60 and HeLa whole cell lysates.
Date published: 2017-02-06
Rated 5 out of 5 by from Produced nice Western blot data of Rho GDIProduced nice Western blot data of Rho GDI expression in K-562 and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-10-06
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Rho GDIα 抗体 (G-2) is rated 4.8 out of 5 by 6.
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