Date published: 2025-9-7
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Rheb Antibody (B-12) - Western Blotting - Image 354931
Rheb 抗体 (B-12): sc-271509. Jurkat (A), A-431 (B), PC-12 (C) 和 HeLa (D) 全细胞裂解液中 Rheb 表达的 Western 印迹分析.
Rheb 抗体 (B-12): sc-271509. Jurkat (A), A-431 (B), PC-12 (C) 和 HeLa (D) 全细胞裂解液中 Rheb 表达的 Western 印迹分析.

Rheb 抗体 (B-12): sc-271509

4.3(12)
寫評論提問

说明书
  • Rheb 抗体 B-12 是小鼠单克隆 IgG2b κ，Rheb抗体, 在12篇文献中引用，规格为200 µg/ml
  • 针对human起源的Rheb内部区域内的氨基酸41-110进行
  • Rheb 抗体 (B-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Rheb
  • 抗Rheb抗体（B-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rheb (B-12): sc-271509的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Rheb Antibody (B-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Rheb Antibody (B-12) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Rheb抗体，包括RhebL1

Rheb抗体（B-12）是一种IgG2b κ小鼠单克隆Rheb抗体（也被指定为RHEB2抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Rheb蛋白。Rheb抗体（B-12）既可作为非偶联的抗Rheb抗体形式提供，也可作为多种偶联形式的抗Rheb抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。H-、K-和N-Ras代表了一类小G蛋白的原型成员，这类蛋白在各种人类肿瘤中经常被激活至致癌状态。这种激活是由于其编码序列中第12位或第61位的点突变引起的。这些突变导致这些蛋白质被持续性地转化为与其活性GTP结合的状态，而不是与非活性GDP结合的状态。相关的人类R-Ras基因最初是通过低严谨性杂交方法克隆的。已证明R-Ras的第38位或第87位突变体（类似于H-Ras中的第12位和第61位）能够激活致癌功能。处于活性GTP结合状态的Ras p21与Raf-1结合，从而激活MAP激酶信号级联。Ras家族的另一个成员Rheb（Ras相关GTP结合蛋白）也与Raf-1相互作用。这种相互作用被生长因子和增加cAMP水平的试剂所增强。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rheb 抗体 (B-12) 参考文献:

  1. Rheb家族GTP酶研究的最新进展。  |  Heard, JJ., et al. 2014. Cell Signal. 26: 1950-7. PMID: 24863881
  2. 人类癌症中的 ras 致癌基因：综述。  |  Bos, JL. 1989. Cancer Res. 49: 4682-9. PMID: 2547513
  3. 与 ras 原癌基因密切相关的新基因--人类和鼠类 R-ras 基因的结构。  |  Lowe, DG., et al. 1987. Cell. 48: 137-46. PMID: 3098437
  4. ras 基因。  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
  5. 人类 R-ras 基因产品的异源表达和特征描述。  |  Lowe, DG. and Goeddel, DV. 1987. Mol Cell Biol. 7: 2845-56. PMID: 3313005
  6. AAV1-Rheb(S16H)转导治疗神经退行性疾病的治疗潜力。  |  Nam, Y., et al. 2021. Int J Mol Sci. 22: PMID: 33802760
  7. Rheb调节核mTORC1活性不依赖于法尼基化。  |  Zhong, Y., et al. 2022. Cell Chem Biol. 29: 1037-1045.e4. PMID: 35294906
  8. CREB3L4通过促进RHEB-mTORC1信号传导途径，促进肝细胞癌进展并降低索拉非尼的化疗敏感性。  |  Jiang, Z., et al. 2024. iScience. 27: 108843. PMID: 38303702
  9. 体外通过Ras依赖性和Ras非依赖性机制调节Raf-1和Raf-1突变体。  |  Dent, P., et al. 1995. Mol Cell Biol. 15: 4125-35. PMID: 7623807
  10. R-Ras 能诱导 NIH3T3 细胞发生恶性转化，但不能诱导其形态转化。  |  Cox, AD., et al. 1994. Oncogene. 9: 3281-8. PMID: 7936652
  11. 通过与原型 H-ras 致癌基因类似的点突变激活人类 R-ras。  |  Saez, R., et al. 1994. Oncogene. 9: 2977-82. PMID: 8084601

订购信息

产品名称产品编号规格价格数量收藏夹

Rheb 抗体 (B-12)

sc-271509
200 µg/ml
RMB2377.00

Rheb (B-12): m-IgGκ BP-HRP 套装

sc-521963
200 µg Ab, 40 µg BP
RMB2662.00

Rheb (B-12): m-IgG2b BP-HRP 套装

sc-548989
200 µg Ab; 10 µg BP
RMB2662.00

Rheb 抗体 (B-12) AC

sc-271509 AC
500 µg/ml, 25% agarose
RMB3129.00

Rheb 抗体 (B-12) HRP

sc-271509 HRP
200 µg/ml
RMB2377.00

Rheb 抗体 (B-12) FITC

sc-271509 FITC
200 µg/ml
RMB2482.00

Rheb 抗体 (B-12) PE

sc-271509 PE
200 µg/ml
RMB2580.00

Rheb 抗体 (B-12) Alexa Fluor® 488

sc-271509 AF488
200 µg/ml
RMB2685.00

Rheb 抗体 (B-12) Alexa Fluor® 546

sc-271509 AF546
200 µg/ml
RMB2685.00

Rheb 抗体 (B-12) Alexa Fluor® 594

sc-271509 AF594
200 µg/ml
RMB2685.00

Rheb 抗体 (B-12) Alexa Fluor® 647

sc-271509 AF647
200 µg/ml
RMB2685.00

Rheb 抗体 (B-12) Alexa Fluor® 680

sc-271509 AF680
200 µg/ml
RMB2685.00

Rheb 抗体 (B-12) Alexa Fluor® 790

sc-271509 AF790
200 µg/ml
RMB2685.00

是否有抗-Rheb 抗体 (B-12) 偶联磁珠用于 IP 的文献？

Asked by: 1111
感谢您的提问。使用该抗体发表的文献可在产品页面查询。如果有其他问题，请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your question. Published literature using this antibody is available on the website. If you have any other question, please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Sen Li
Date published: 2025-02-20

抗-Rheb 抗体 (B-12) 可否偶联磁珠用于 IP ？

Asked by: JUNT
感谢您的提问。您可以偶联磁珠用于 IP。 Thank you for your question. You can couple magnetic beads for IP.
Answered by: Zhanglong
Date published: 2024-08-12

I was using this antibody on 2 separate westerns to detect endogenous RHEB in Hep3B, HepG2 and HeLA cell lines. In both occasions, an intense band more than 50 Kda was observed. Given that the predicted size of RHEB is around 20 kD, how is this possible?

Asked by: Siew Lan
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-02-23

Can Rheb (B-12): sc-271509 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Rheb (B-12): sc-271509 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Antiody works for immunoblotting.Antibody works great for immunoblot analysis and is highly specific for Rheb1.
Date published: 2019-02-16
Rated 5 out of 5 by from works well for endogenous Rheb IPThis is the best product I ever used for endogenous Rheb IP. It is very specific.
Date published: 2017-12-31
Rated 4 out of 5 by from Good for WB on recombinant Rat proteinI tested on cells transfected with a recombinant Rat form of Rheb and it worked well (1:500)
Date published: 2017-11-07
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of 5 by from good做的大鼠睾丸组织的western，开始做的一两次还行，但是后面量就没有了，可能挥发了吧，总之，如果量足的话还是不错的
Date published: 2017-07-19
Rated 5 out of 5 by from Worked great for Western and immunoprecipitation.This antibody cleanly identified N-terminally Flag-tagged human Rheb in transiently transfected 293T cell lysates. It also immunoprecipitated Rheb from the same lysates.
Date published: 2017-06-12
Rated 4 out of 5 by from Clear WB bandTested endogenous RheB in HeLa cells, got clear band!
Date published: 2017-06-08
Rated 5 out of 5 by from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-17
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Rheb 抗体 (B-12) is rated 4.3 out of 5 by 12.
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