Date published: 2025-9-8

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RGS5 抗体 (B-4): sc-514184

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说明书
  • RGS5 抗体 B-4 是小鼠单克隆 IgG1 κ,RGS5抗体, 在2篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的RGS5的N-terminus附近的氨基酸28-47之间
  • RGS5 抗体 (B-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 RGS5
  • 抗RGS5抗体(B-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RGS5 (B-4): sc-514184免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 RGS5 抗体 (B-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RGS5 抗体 (B-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

RGS5抗体(B-4)是一种IgG1 κ型小鼠单克隆RGS5抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学(IHC(P))和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的RGS5蛋白。RGS5抗体(B-4)既有非偶联形式的抗RGS5抗体,也有多种偶联形式的抗RGS5抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。G蛋白信号调节因子(RGS蛋白)是一个具有高度多样性、多功能信号蛋白的家族,这些蛋白共享一个保守的120个氨基酸结构域(RGS结构域)。RGS结构域直接与活化的Gα亚基结合,并作为GTP酶激活蛋白(GAPs)来减弱和/或调节由Gα-GTP和Gβγ介导的激素和神经递质受体引发的信号传导。RGS蛋白缩短活化的G蛋白的寿命。血管内皮细胞表达RGS蛋白RGS5,它与毛细血管形态发生相关,因此它是参与毛细血管生长、血管生成以及潜在的中风病理生理学的候选基因。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RGS5 抗体 (B-4) 参考文献:

  1. RGS 蛋白在细胞信号中的新作用。  |  Hepler, JR. 1999. Trends Pharmacol Sci. 20: 376-82. PMID: 10462761
  2. Rgs5的水泡特异性表达:血管成熟过程中PDGF和EDG受体信号的影响  |  Cho, H., et al. 2003. FASEB J. 17: 440-2. PMID: 12514120
  3. G 蛋白信号调节器:对心血管系统信号产生影响的多功能蛋白质。  |  Wieland, T. and Mittmann, C. 2003. Pharmacol Ther. 97: 95-115. PMID: 12559385
  4. 血小板衍生生长因子-B缺陷小鼠胚胎的转录谱分析发现 RGS5 是周细胞和血管平滑肌细胞的新标记。  |  Bondjers, C., et al. 2003. Am J Pathol. 162: 721-9. PMID: 12598306
  5. 人类肾细胞癌肿瘤血管中 G 蛋白信号调节蛋白 5 (RGS5) 的表达。  |  Furuya, M., et al. 2004. J Pathol. 203: 551-8. PMID: 15095478
  6. G 蛋白信号调节器 5 标志着外周动脉平滑肌细胞,并在动脉粥样硬化斑块中下调。  |  Li, J., et al. 2004. J Vasc Surg. 40: 519-28. PMID: 15337883
  7. 鉴定人类眼组织中 RGS5 mRNA 的新型替代剪接变体。  |  Liang, Y., et al. 2005. FEBS J. 272: 791-9. PMID: 15670159
  8. β肾上腺素能受体介导的心房特异性 RGS5 上调。  |  Jean-Baptiste, G., et al. 2005. Life Sci. 76: 1533-45. PMID: 15680317
  9. GPSM3对RGS5 GAP活性的调节。  |  Zhao, P. and Chidiac, P. 2015. Mol Cell Biochem. 405: 33-40. PMID: 25842189
  10. 血管平滑肌细胞中由高血压诱发的 RhoA 活性需要 RGS5。  |  Arnold, C., et al. 2018. FASEB J. 32: 2021-2035. PMID: 29208700
  11. Resmetirom通过抑制STAT3和NF-κB信号通路改善RGS5依赖性NASH模型小鼠。  |  Wang, X., et al. 2023. Int J Mol Sci. 24: PMID: 36982915
  12. 心肌细胞表达十种 RGS 蛋白的 mRNA:心室肌细胞和培养心房中 RGS mRNA 表达的变化。  |  Kardestuncer, T., et al. 1998. FEBS Lett. 438: 285-8. PMID: 9827562

订购信息

产品名称产品编号规格价格数量收藏夹

RGS5 抗体 (B-4)

sc-514184
200 µg/ml
RMB2377.00

RGS5 (B-4): m-IgG Fc BP-HRP 套装

sc-527861
200 µg Ab; 10 µg BP
RMB2662.00

RGS5 (B-4): m-IgG1 BP-HRP 套装

sc-533234
200 µg Ab; 20 µg BP
RMB2662.00

RGS5 抗体 (B-4) AC

sc-514184 AC
500 µg/ml, 25% agarose
RMB3129.00

RGS5 抗体 (B-4) HRP

sc-514184 HRP
200 µg/ml
RMB2377.00

RGS5 抗体 (B-4) FITC

sc-514184 FITC
200 µg/ml
RMB2482.00

RGS5 抗体 (B-4) PE

sc-514184 PE
200 µg/ml
RMB2580.00

RGS5 抗体 (B-4) Alexa Fluor® 488

sc-514184 AF488
200 µg/ml
RMB2685.00

RGS5 抗体 (B-4) Alexa Fluor® 546

sc-514184 AF546
200 µg/ml
RMB2685.00

RGS5 抗体 (B-4) Alexa Fluor® 594

sc-514184 AF594
200 µg/ml
RMB2685.00

RGS5 抗体 (B-4) Alexa Fluor® 647

sc-514184 AF647
200 µg/ml
RMB2685.00

RGS5 抗体 (B-4) Alexa Fluor® 680

sc-514184 AF680
200 µg/ml
RMB2685.00

RGS5 抗体 (B-4) Alexa Fluor® 790

sc-514184 AF790
200 µg/ml
RMB2685.00

RGS5 (B-4) 中和勝肽

sc-514184 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-514184 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Western blot application on ratI have tested protein concentration from 25ug, 50ug, and 60ug and antibody from 1:1000 to 1:500 for western blot. however, no protein band was observed
Date published: 2024-10-23
Rated 4 out of 5 by from Great for WB!This antibody works well for WB in mouse primary muscle cells.
Date published: 2018-03-07
Rated 5 out of 5 by from Great features!I got the sample a month ago, and try it with WB, the band is very sharp and specific! Recommend it!
Date published: 2018-02-27
Rated 2 out of 5 by from No bands under 30 kDa?Rgs5 gene transfected cell lysates. No bands under 30 kDa in our western blotting experiment.
Date published: 2017-09-29
Rated 1 out of 5 by from No positive signals by IF stainingI have tested this antibody in our frozen mouse brain section, however it did not show any positive signal. fixatives: 4% PFA, blocking buffer: 5% normal goat serum & 0.3% triton-X100 1 Ab incubation:4 degrees Celsius overnight.
Date published: 2017-07-12
Rated 5 out of 5 by from Satisfactory Western Blot data of RGS5 expressionSatisfactory Western Blot data of RGS5 expression in non-transfected and mouse RGS5 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-16
Rated 5 out of 5 by from Nice Western Blot data of RGS5 expressionNice Western Blot data of RGS5 expression in Hep G2 whole cell lysate. -SCBT QC
Date published: 2013-02-06
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RGS5 抗体 (B-4) is rated 3.4 out of 5 by 7.
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