Date published: 2025-9-7

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Rex-1 抗体 (E-11): sc-377095

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说明书
  • Rex-1 抗体 E-11 是小鼠单克隆 IgG1 κ,Rex-1抗体, 在4篇文献中引用,规格为200 µg/ml
  • 与氨基酸序列C-terminal(h)对应的多肽序列(Rex-1的human起源)
  • Rex-1 抗体 (E-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Rex-1
  • 抗Rex-1抗体(E-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-377095 X, 200 µg/0.1 ml)
  • 关于如何获取Rex-1 (E-11): sc-377095免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Rex-1 抗体 (E-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Rex-1 抗体 (E-11) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Rex-1抗体(E-11)是一种IgG1 κ小鼠单克隆Rex-1抗体(也称为Rex-1抗体),可通过蛋白质印迹法(WB)、免疫沉淀法(IP)、免疫荧光法(IF)、免疫组织化学法(IHC(P))和酶联免疫吸附测定法(ELISA)检测到小鼠、大鼠和人源的Rex-1蛋白。Rex-1抗体(E-11)既有非偶联的抗Rex-1抗体形式,也有多种偶联形式的抗Rex-1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Rex-1(表达减少),也称为锌指蛋白42(ZFP42),是一种酸性锌指蛋白。Rex-1包含四个锌指核酸结合基序的重复序列和一个潜在的酸性激活结构域,表明它是一种调节蛋白。Rex-1定位于细胞核,并在胚胎干细胞(ES)和未分化的小鼠F9畸胎瘤细胞中高度表达。在转录水平上,当通过添加维甲酸(RA)诱导F9细胞分化时,Rex-1的表达降低,而E1A可增强Rex-1的抑制。Oct-3/4转录因子可以根据细胞环境激活或抑制Rex-1启动子,而Oct-6可以降低Rex-1的表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rex-1 抗体 (E-11) 参考文献:

  1. 胚胎植入前的人类胚胎和胚胎干细胞在特定阶段胚胎抗原的表达上具有可比性。  |  Henderson, JK., et al. 2002. Stem Cells. 20: 329-37. PMID: 12110702
  2. 尼古丁对小鼠胚胎干细胞 Oct-4 和 Rex-1 表达的影响  |  Zhang, H., et al. 2005. Reprod Toxicol. 19: 473-8. PMID: 15749260
  3. 从中国人口中提取的人类胚胎干细胞系。  |  Fang, ZF., et al. 2005. Cell Res. 15: 394-400. PMID: 15916726
  4. 白血病抑制因子是无饲养细胞无血清培养基中多能小鼠胚胎干细胞的抗凋亡有丝分裂原。  |  Furue, M., et al. 2005. In Vitro Cell Dev Biol Anim. 41: 19-28. PMID: 15926856
  5. Nanog和Sox2对多能性标记Rex-1的调控  |  Shi, W., et al. 2006. J Biol Chem. 281: 23319-25. PMID: 16714766
  6. 推测多能性标志物UTF-1和REX-1在人类成人睾丸和生殖细胞肿瘤中表达。  |  Kristensen, DM., et al. 2008. Hum Reprod. 23: 775-82. PMID: 18281244
  7. Rex-1 与目的基因的关联支持其与多聚酶功能的相互作用。  |  Garcia-Tuñon, I., et al. 2011. Stem Cell Res. 7: 1-16. PMID: 21530438
  8. IL-6调节应激诱导的人类脱落牙齿干细胞中Rex-1的表达。  |  Govitvattana, N., et al. 2013. Oral Dis. 19: 673-82. PMID: 23279369
  9. REX-1 抑制 RASSF1a 并激活 MEK/ERK 通路,促进前列腺癌的肿瘤发生  |  Liu, W., et al. 2021. Mol Cancer Res. 19: 1666-1675. PMID: 34183450
  10. 八聚体基团有助于视黄酸调控的锌指基因Rex-1(Zfp-42)在F9畸胎瘤细胞中的表达。  |  Hosler, BA., et al. 1993. Mol Cell Biol. 13: 2919-28. PMID: 8474450
  11. Rex-1是一个编码在早期胚胎中表达的转录因子的基因,它通过Oct-3/4和Oct-6与一个八聚体位点的结合以及一个新蛋白Rox-1与邻近位点的结合进行调控。  |  Ben-Shushan, E., et al. 1998. Mol Cell Biol. 18: 1866-78. PMID: 9528758
  12. 视黄酸可迅速减少 F9畸胎癌细胞中含有锌指图案的基因 REX-1 的表达。  |  Hosler, BA., et al. 1989. Mol Cell Biol. 9: 5623-9. PMID: 2511439

订购信息

产品名称产品编号规格价格数量收藏夹

Rex-1 抗体 (E-11)

sc-377095
200 µg/ml
RMB2377.00

Rex-1 (E-11): m-IgG Fc BP-HRP 套装

sc-529973
200 µg Ab; 10 µg BP
RMB2662.00

Rex-1 (E-11): m-IgGκ BP-HRP 套装

sc-523185
200 µg Ab, 40 µg BP
RMB2662.00

Rex-1 (E-11): m-IgG1 BP-HRP 套装

sc-543797
200 µg Ab; 20 µg BP
RMB2662.00

Rex-1 抗体 (E-11) X

sc-377095 X
200 µg/0.1 ml
RMB2377.00

Rex-1 抗体 (E-11) AC

sc-377095 AC
500 µg/ml, 25% agarose
RMB3129.00

Rex-1 抗体 (E-11) HRP

sc-377095 HRP
200 µg/ml
RMB2377.00

Rex-1 抗体 (E-11) FITC

sc-377095 FITC
200 µg/ml
RMB2482.00

Rex-1 抗体 (E-11) PE

sc-377095 PE
200 µg/ml
RMB2580.00

Rex-1 抗体 (E-11) Alexa Fluor® 488

sc-377095 AF488
200 µg/ml
RMB2685.00

Rex-1 抗体 (E-11) Alexa Fluor® 546

sc-377095 AF546
200 µg/ml
RMB2685.00

Rex-1 抗体 (E-11) Alexa Fluor® 594

sc-377095 AF594
200 µg/ml
RMB2685.00

Rex-1 抗体 (E-11) Alexa Fluor® 647

sc-377095 AF647
200 µg/ml
RMB2685.00

Rex-1 抗体 (E-11) Alexa Fluor® 680

sc-377095 AF680
200 µg/ml
RMB2685.00

Rex-1 抗体 (E-11) Alexa Fluor® 790

sc-377095 AF790
200 µg/ml
RMB2685.00

hi, Can I use Rex-1 Antibody (E-11): sc-377095 for Immunofluor? Thanks, Mohammed

Asked by: MOHAMMED
Thank you for your question. Yes, immunofluorescence (IF) is a recommended application for this antibody.
Answered by: Technical Support
Date published: 2019-01-26

What is a good positive control to use anti-Rex antibody for western blots?

Asked by: sudipta
Thank you for your question. We recommend PC-3 or DU 145 whole cell lysates as positive controls for this target. Please contact Technical Service if you have any further questions.
Answered by: Technical Support
Date published: 2019-01-06

I am using Rex-1 (E-11): sc-377095 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from no stainingNo staining after immunofluorescence on PFA-fixed cells at 1:1000 antibody dilution.
Date published: 2019-04-18
Rated 1 out of 5 by from Nonreactive in mouse IFNo fluorescent signal in (PFA-fixed) frozen mouse testis
Date published: 2018-04-03
Rated 5 out of 5 by from Produced publishable IF data in mouseThis Rex-1 monoclonal antibody was referenced in a couple publications for the use of IF on mouse samples
Date published: 2017-02-08
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclearProduced exceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded nasopharynx tissue. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by from Satisfactory Western Blot data of RexSatisfactory Western Blot data of Rex-1 expression in PC-3 and DU 145 nuclear extracts. -SCBT QC
Date published: 2014-05-14
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Rex-1 抗体 (E-11) is rated 3.4 out of 5 by 5.
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