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Reptin 52 Antibody (B-5) - Western Blotting - Image 358571
Reptin 52 抗体 (B-5): sc-374135. Jurkat (A), Hep G2 (B), c4 (C), NIH/3T3 (D), KNRK (E) 和 RPE-J (F) 全细胞裂解液中 Reptin 52 表达的 Western 印迹分析.
Reptin 52 抗体 (B-5): sc-374135. Jurkat (A), Hep G2 (B), c4 (C), NIH/3T3 (D), KNRK (E) 和 RPE-J (F) 全细胞裂解液中 Reptin 52 表达的 Western 印迹分析.

Reptin 52 抗体 (B-5): sc-374135

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说明书
  • Reptin 52 抗体 B-5 是小鼠单克隆 IgG2a κ，Reptin 52抗体, 在5篇文献中引用，规格为200 µg/ml
  • 针对human起源的Reptin 52内部区域内的氨基酸134-295进行
  • Reptin 52 抗体 (B-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Reptin 52; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗Reptin 52抗体（B-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-374135 X, 200 µg/0.1 ml)
  • 关于如何获取Reptin 52 (B-5): sc-374135的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是Reptin 52 Antibody (B-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Reptin 52 Antibody (B-5) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Reptin抗体

Reptin 52抗体（B-5）是一种IgG2a κ小鼠单克隆Reptin 52抗体（也称为Reptin 52抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类的Reptin 52蛋白。Reptin 52抗体（B-5）既可以是非偶联的抗Reptin 52抗体形式，也可以是多种偶联形式的抗Reptin 52抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Pontin 52是一种主要表达于细胞核同时也存在于细胞质中的核基质蛋白。它在全细胞的核质中表达，但不存在于核仁中。Pontin 52，也称为E. coli RuvB样1蛋白的RUVBL1或NMP 238，是大鼠TIP49的人类同源物。Pontin 52包含一个ATP酶/解旋酶基序，可能代表一类由转录激活结构域招募的辅因子，这些辅因子在多种途径中发挥作用。例如，在体内，Pontin 52与Myc和Reptin 52（一种与Pontin 52相关的蛋白质，也称为RUVBL2）形成复合物。Pontin 52与Myc的相互作用依赖于Myc结构域，该结构域对致癌活性至关重要，这表明功能性Pontin 52是Myc致癌转化的重要介质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Reptin 52 抗体 (B-5) 参考文献:

  1. ATP酶/螺旋酶复合物是c-Myc致癌转化的重要辅助因子。  |  Wood, MA., et al. 2000. Mol Cell. 5: 321-30. PMID: 10882073
  2. RuvBl2与Ets2合作转录调控结肠癌中的hTERT。  |  Flavin, P., et al. 2011. FEBS Lett. 585: 2537-44. PMID: 21763315
  3. 通过 BioID 鉴定线粒体 DNA 聚合酶 gamma 的相互作用组，发现 Ruvbl2 定位于线粒体。  |  Liyanage, SU., et al. 2017. Mitochondrion. 32: 31-35. PMID: 27845271
  4. AAA+ATP酶RUVBL2对急性髓性白血病中c-MYB的致癌功能至关重要。  |  Armenteros-Monterroso, E., et al. 2019. Leukemia. 33: 2817-2829. PMID: 31138842
  5. RUVBL1-RUVBL2-ZNHIT2 的冷冻电镜，这是一个与前核糖核酸加工剪接因子 8 相互作用的复合物。  |  Serna, M., et al. 2022. Nucleic Acids Res. 50: 1128-1146. PMID: 34951455
  6. 解密人类 DPCD 蛋白与 RUVBL1/RUVBL2 AAA-ATPases 复合物的细胞和分子决定因素。  |  Dos Santos Morais, R., et al. 2022. J Mol Biol. 434: 167760. PMID: 35901867
  7. 新型 LINC02321 通过调节 RUVBL2 促进膀胱癌细胞增殖并降低顺铂敏感性。  |  Lu, C., et al. 2024. Transl Oncol. 45: 101962. PMID: 38677015
  8. 敲除 RUVBL2 可改善 hnRNPA2/B1 应激颗粒动态，从而抑制老年轻度认知障碍大鼠围手术期的神经认知障碍。  |  Wang, Z., et al. 2024. Aging Cell. e14418. PMID: 39610020
  9. TIP49 与细菌 DNA 螺旋酶 RuvB 同源，是人类的一种自身抗原。  |  Makino, Y., et al. 1998. Biochem Biophys Res Commun. 245: 819-23. PMID: 9588198
  10. 真核生物 RuvB 样蛋白（RUVBL1）对生长至关重要。  |  Qiu, XB., et al. 1998. J Biol Chem. 273: 27786-93. PMID: 9774387
  11. 鉴定和描述普遍存在的核基质蛋白 NMP 238。  |  Holzmann, K., et al. 1998. Biochem Biophys Res Commun. 252: 39-45. PMID: 9813143
  12. Pontin52 是 beta-catenin 的相互作用伙伴，能与 TATA 盒结合蛋白结合。  |  Bauer, A., et al. 1998. Proc Natl Acad Sci U S A. 95: 14787-92. PMID: 9843967

订购信息

产品名称产品编号规格价格数量收藏夹

Reptin 52 抗体 (B-5)

sc-374135
200 µg/ml
RMB2377.00

Reptin 52 (B-5): m-IgG Fc BP-HRP 套装

sc-525937
200 µg Ab; 10 µg BP
RMB2662.00

Reptin 52 抗体 (B-5) X

sc-374135 X
200 µg/0.1 ml
RMB2377.00

Reptin 52 抗体 (B-5) AC

sc-374135 AC
500 µg/ml, 25% agarose
RMB3129.00

Reptin 52 抗体 (B-5) HRP

sc-374135 HRP
200 µg/ml
RMB2377.00

Reptin 52 抗体 (B-5) FITC

sc-374135 FITC
200 µg/ml
RMB2482.00

Reptin 52 抗体 (B-5) PE

sc-374135 PE
200 µg/ml
RMB2580.00

Reptin 52 抗体 (B-5) Alexa Fluor® 488

sc-374135 AF488
200 µg/ml
RMB2685.00

Reptin 52 抗体 (B-5) Alexa Fluor® 546

sc-374135 AF546
200 µg/ml
RMB2685.00

Reptin 52 抗体 (B-5) Alexa Fluor® 594

sc-374135 AF594
200 µg/ml
RMB2685.00

Reptin 52 抗体 (B-5) Alexa Fluor® 647

sc-374135 AF647
200 µg/ml
RMB2685.00

Reptin 52 抗体 (B-5) Alexa Fluor® 680

sc-374135 AF680
200 µg/ml
RMB2685.00

Reptin 52 抗体 (B-5) Alexa Fluor® 790

sc-374135 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with Reptin 52 (B-5): sc-374135 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Good choice for IFI used this antibody at a dilution 1:200 in pancreatic cancer cells. We can got sensitive and repeatable results. I would recommend it to colleagues.
Date published: 2023-08-03
Rated 4 out of 5 by from clean antibodyin compare with other brand, SantaCruz antibody has a clean and nice band
Date published: 2018-07-03
Rated 5 out of 5 by from GoodVery nice shopping experience! Agents are all really great!
Date published: 2017-11-14
Rated 5 out of 5 by from Works great for WB/IF1:1000 for WB, 1:200 for IF. Works great with PF fixed cells, 1 hr incubation at room temp.
Date published: 2017-08-23
Rated 5 out of 5 by from PF fixation works bestAsynchronous HeLa were either paraformaldehyde or methanol fixed, immunofluorescently labeled and counterstained with DAPI. PF fixation works best as it only requires a 1hr incubation time where as MeOH fixation requires an o/n incubation with the primary antibody. The antibody gives a predominantly nuclear focal staining pattern in interphase cels.
Date published: 2017-02-18
Rated 5 out of 5 by from Superb immunofluorescence nuclear and cytoplasmicSuperb immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-13
Rated 5 out of 5 by from Nice Western Blot data of Reptin 52 expressionNice Western Blot data of Reptin 52 expression in non-transfected 293T, human Reptin 52 transfected 293T and K-562 whole cell lysates and mouse kidney and human kidney tissue extracts. -SCBT QC
Date published: 2013-01-25
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Reptin 52 抗体 (B-5) is rated 4.7 out of 5 by 7.
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