Date published: 2025-9-8
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Renin Antibody (B-12) - Western Blotting - Image 356177
肾素抗体 (B-12): sc-133145. HeLa (A), Hep G2 (B), AMJ2-C8 (C) 和 KNRK (D) 全细胞裂解液中肾素表达的 Western 印迹分析.
肾素抗体 (B-12): sc-133145. HeLa (A), Hep G2 (B), AMJ2-C8 (C) 和 KNRK (D) 全细胞裂解液中肾素表达的 Western 印迹分析.

Renin 抗体 (B-12): sc-133145

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寫評論提問

说明书
  • Renin 抗体 B-12 是小鼠单克隆 IgG1 κ，Renin抗体, 在23篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Renin的氨基酸116-220
  • Renin 抗体 (B-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 precursor and mature Renin
  • 抗Renin抗体（B-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Renin (B-12): sc-133145的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Renin 抗体 (B-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Renin 抗体 (B-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

肾素抗体（B-12）是一种IgG1 κ小鼠单克隆肾素抗体（也称为血管紧张素原酶抗体、肾素-血管紧张素-醛固酮系统（RAAS）相关抗体、血管紧张素形成酶抗体、高尿酸血症肾病、家族性青少年2型抗体或HNFJ2抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的肾素蛋白。肾素抗体（B-12）既有非偶联形式的抗肾素抗体，也有多种偶联形式的抗肾素抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肾素是一种高度特异性的内肽酶，可介导循环底物血管紧张素原的裂解，产生血管紧张素I。然后，血管紧张素转化酶I（ACE）完成从血管紧张素I到血管紧张素II的转化，这在调节电解质平衡和血压方面具有重要意义。交感神经刺激（β1-肾上腺素能受体）、肾动脉低血压和肾远端小管钠传递的减少都会发出肾素释放的信号。肾素-血管紧张素系统（RAS）对于调节血容量、动脉压以及维持正常的心脏和血管功能至关重要。肾素由肾脏肾小球旁器（JGA）中的修饰平滑肌细胞合成并分泌。尽管肾素在其他组织（包括大脑）中的表达已得到验证，但其可能发挥的稳态作用尚有待确定。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Renin 抗体 (B-12) 参考文献:

  1. 遗传性血管紧张素原减少的小鼠体内的平衡主要是通过肾素生成细胞数量的增加来实现的。  |  Kim, HS., et al. 1999. J Biol Chem. 274: 14210-7. PMID: 10318840
  2. 肾素与肾脏  |  Chonko, AM., et al. 1975. Nephron. 15: 279-305. PMID: 1101089
  3. 微血管血管生成与肾素-血管紧张肽系统。  |  Greene, AS. and Amaral, SL. 2002. Curr Hypertens Rep. 4: 56-62. PMID: 11790293
  4. 肾素血管紧张肽系统与肾脏发育。  |  Matsusaka, T., et al. 2002. Annu Rev Physiol. 64: 551-61. PMID: 11826279
  5. 肾素基因的表达：开关和手指。  |  Morris, BJ., et al. 2001. Clin Exp Pharmacol Physiol. 28: 1044-7. PMID: 11903312
  6. HADHB, HuR和CP1与人肾素mRNA远端3'-非翻译区结合，并以不同方式调节肾素的表达。  |  Adams, DJ., et al. 2003. J Biol Chem. 278: 44894-903. PMID: 12933794
  7. 启动子区域中人类肾素基因表达的候选顺式元件。  |  Konoshita, T., et al. 2004. J Cell Biochem. 93: 327-36. PMID: 15368359
  8. 肾素依赖性高矿物皮质激素症。  |  Young, WF. and Hogan, MJ. 1994. Trends Endocrinol Metab. 5: 97-106. PMID: 18407194
  9. 肾素的生理学  |  Castrop, H., et al. 2010. Physiol Rev. 90: 607-73. PMID: 20393195
  10. 克隆 cDNA 在哺乳动物细胞中表达的人促肾素的特征。  |  Fritz, LC., et al. 1986. Proc Natl Acad Sci U S A. 83: 4114-8. PMID: 3520565
  11. 肾素和肾素阻断剂对补体活性没有作用。  |  Zhang, Y., et al. 2024. Kidney Int. 105: 328-337. PMID: 38008161
  12. Ren-1 和 Ren-2 基因座在小鼠肾脏中表达。  |  Field, LJ. and Gross, KW. 1985. Proc Natl Acad Sci U S A. 82: 6196-200. PMID: 3898081

订购信息

产品名称产品编号规格价格数量收藏夹

Renin 抗体 (B-12)

sc-133145
200 µg/ml
RMB2377.00

Renin (B-12): m-IgG Fc BP-HRP 套装

sc-527095
200 µg Ab; 10 µg BP
RMB2662.00

Renin (B-12): m-IgG1 BP-HRP 套装

sc-532468
200 µg Ab; 20 µg BP
RMB2662.00

Renin 抗体 (B-12) AC

sc-133145 AC
500 µg/ml, 25% agarose
RMB3129.00

Renin 抗体 (B-12) HRP

sc-133145 HRP
200 µg/ml
RMB2377.00

Renin 抗体 (B-12) FITC

sc-133145 FITC
200 µg/ml
RMB2482.00

Renin 抗体 (B-12) PE

sc-133145 PE
200 µg/ml
RMB2580.00

Renin 抗体 (B-12) Alexa Fluor® 488

sc-133145 AF488
200 µg/ml
RMB2685.00

Renin 抗体 (B-12) Alexa Fluor® 546

sc-133145 AF546
200 µg/ml
RMB2685.00

Renin 抗体 (B-12) Alexa Fluor® 594

sc-133145 AF594
200 µg/ml
RMB2685.00

Renin 抗体 (B-12) Alexa Fluor® 647

sc-133145 AF647
200 µg/ml
RMB2685.00

Renin 抗体 (B-12) Alexa Fluor® 680

sc-133145 AF680
200 µg/ml
RMB2685.00

Renin 抗体 (B-12) Alexa Fluor® 790

sc-133145 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-02-08
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Rated 4 out of 5 by from works on FFPEClear control cells in the kidney (same position of those seen using a RNA scope probe for Renin), but large amount of aspecific stain in other FFPE human tissues like ileum.
Date published: 2022-09-02
Rated 5 out of 5 by from gute Ergebnisse im Western Blot von humanem PlasmaProbe am 2.11.17 erhalten, da der AK Renin G-18 nicht mehr produziert wird. Musste etwas austesten, aber bin sehr zufrieden mit dem neuen AK.
Date published: 2017-12-13
Rated 5 out of 5 by from Excellent resultRenin antibody stains very brightly in Mouse cells,simple operation and short cycle. It is very clear in western bloting.
Date published: 2017-09-04
Rated 4 out of 5 by from Vitamin D receptor deficitWe hypothesized that lack of VDR would enhance p53 expression in podocytes through down regulation of SIRT1; the former would enhance the transcription of angiotensinogen (Agt) and angiotensinogen II type 1 receptor (AT1R) leading to the activation of RAS. Renal tissues of VDR mutant (M) mice displayed increased expression of p53, Agt, renin, and AT1R. In vitro studies, VDR knockout podocytes not only displayed up regulation p53 but also displayed enhanced expression of Agt, renin and AT1R. VDR deficient podocytes also displayed an increase in mRNA expression for p53, Agt, renin, and AT1R.
Date published: 2017-08-11
Rated 5 out of 5 by from rat spleen tissueThis antibody shows hight express in rat tissue. I tried it detect this sample from dilution 1/1000, I obtained specific band.
Date published: 2017-08-11
Rated 5 out of 5 by from It worked well.Clean bands observed in we stern blot analysis of p22-phox expression in RAW 264.7, THP-1 and SK-N-MC whole cell lysates.
Date published: 2017-02-07
Rated 5 out of 5 by from Good specificityRenin antibody worked well in the mouse samples,strong single band in WB in mouse Peritoneal macrophages,good specificity. got a pretty good impression of our lab.
Date published: 2017-02-04
Rated 5 out of 5 by from Great monoclonal for IF and WBSpecific staining in IF and solid single bands in Western blot make this a great choice for Renin detection.
Date published: 2017-01-26
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Renin 抗体 (B-12) is rated 4.7 out of 5 by 12.
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