Date published: 2025-9-8

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Reelin 抗体 (E-5): sc-25346

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说明书
  • Reelin 抗体 E-5 是小鼠单克隆 IgG2a κ,Reelin抗体, 在27篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Reelin的氨基酸3239-3460
  • Reelin 抗体 (E-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Reelin
  • Reelin 抗体 (E-5) 可偶联 琼脂糖 用于 IP; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Reelin (E-5): sc-25346免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 Reelin 抗体 (E-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Reelin 抗体 (E-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Reelin抗体(E-5)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附 酶联免疫吸附试验(ELISA)。抗Reelin抗体(E-5)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Reelin,也称为Reln,是一种主要由前脑的Cajal-Retzius细胞和小脑的颗粒神经元分泌的大型糖蛋白,在中央神经系统的发育中起着至关重要的作用。Reelin调节涉及Disabled-1(Dab1)的信号通路,后者对于大脑发育过程中神经元的正确定位和迁移至关重要,可维持大脑的分层结构。Reelin基因的突变与严重的神经系统疾病有关,正如"reeler"小鼠模型所证明的那样,该模型表现出明显的脑结构紊乱,导致运动协调障碍、震颤和共济失调。Reelin的表达在整个发育过程中是变化的,这凸显了Reelin在神经发育过程中的重要性及其对神经精神疾病的潜在影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Reelin 抗体 (E-5) 参考文献:

  1. 髓鞘蛋白信号在阿尔茨海默病中的作用  |  Yu, NN., et al. 2016. Mol Neurobiol. 53: 5692-700. PMID: 26491027
  2. Reelin 蛋白质溶解影响与突触正常功能和神经退行性变相关的信号传导  |  Lussier, AL., et al. 2016. Front Cell Neurosci. 10: 75. PMID: 27065802
  3. 大脑发育和成熟过程中 Reelin 的功能、作用机制和信号通路  |  Jossin, Y. 2020. Biomolecules. 10: PMID: 32604886
  4. 甲状腺激素调节神经精神疾病中的辘in表达。  |  Su, Y., et al. 2022. Can J Physiol Pharmacol.. PMID: 36166833
  5. 由SMARCD3-DAB1-Reelin信号介导的神经发育表观遗传程序被劫持以促进髓母细胞瘤转移。  |  Zou, H., et al. 2023. Nat Cell Biol. 25: 493-507. PMID: 36849558
  6. 血栓素原1和Reelin通过Vldlr调节心脏生长和修复。  |  Pei, L., et al. 2024. Basic Res Cardiol. 119: 169-192. PMID: 38147128
  7. 一种与小鼠突变体雷勒中被删除的细胞外基质蛋白有关的蛋白质。  |  D'Arcangelo, G., et al. 1995. Nature. 374: 719-23. PMID: 7715726
  8. 在成年大鼠的皮层和海马中,合成γ-氨基丁酸的神经元优先表达 Reelin。  |  Pesold, C., et al. 1998. Proc Natl Acad Sci U S A. 95: 3221-6. PMID: 9501244
  9. 胰岛素在控制大脑发育中的作用  |  Curran, T. and D'Arcangelo, G. 1998. Brain Res Brain Res Rev. 26: 285-94. PMID: 9651544
  10. 在控制哺乳动物大脑层状组织的信号通路中,Disabled-1作用于Reelin的下游。  |  Rice, DS., et al. 1998. Development. 125: 3719-29. PMID: 9716537
  11. BDNF调节大脑皮层中缰绳蛋白的表达和Cajal-Retzius细胞的发育。  |  Ringstedt, T., et al. 1998. Neuron. 21: 305-15. PMID: 9728912
  12. 发育中和成年小鼠前脑中Relin mRNA表达的区域和细胞模式  |  Alcántara, S., et al. 1998. J Neurosci. 18: 7779-99. PMID: 9742148

订购信息

产品名称产品编号规格价格数量收藏夹

Reelin 抗体 (E-5)

sc-25346
200 µg/ml
RMB2377.00

Reelin (E-5): m-IgG Fc BP-HRP 套装

sc-528408
200 µg Ab; 10 µg BP
RMB2662.00

Reelin (E-5): m-IgGκ BP-HRP 套装

sc-520781
200 µg Ab, 40 µg BP
RMB2662.00

Reelin (E-5): m-IgG2a BP-HRP 套装

sc-547088
200 µg Ab; 10 µg BP
RMB2662.00

Reelin 抗体 (E-5) AC

sc-25346 AC
500 µg/ml, 25% agarose
RMB3129.00

Reelin 抗体 (E-5) FITC

sc-25346 FITC
200 µg/ml
RMB2482.00

Reelin 抗体 (E-5) PE

sc-25346 PE
200 µg/ml
RMB2580.00

Reelin 抗体 (E-5) Alexa Fluor® 488

sc-25346 AF488
200 µg/ml
RMB2685.00

Reelin 抗体 (E-5) Alexa Fluor® 546

sc-25346 AF546
200 µg/ml
RMB2685.00

Reelin 抗体 (E-5) Alexa Fluor® 594

sc-25346 AF594
200 µg/ml
RMB2685.00

Reelin 抗体 (E-5) Alexa Fluor® 647

sc-25346 AF647
200 µg/ml
RMB2685.00

Reelin 抗体 (E-5) Alexa Fluor® 680

sc-25346 AF680
200 µg/ml
RMB2685.00

Reelin 抗体 (E-5) Alexa Fluor® 790

sc-25346 AF790
200 µg/ml
RMB2685.00

Do you have any FACS analyis or data of this ab used in Flow cytometry?

Asked by: Andres
Thank you for your question. Reelin Antibody (E-5): sc-25346 can be used in flow cytometry and is available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM. Please contact our technical service team directly for additional information.
Answered by: Tech Support Europe
Date published: 2023-12-27

Can Reelin (E-5): sc-25346 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Reelin (E-5): sc-25346 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Works but with strong backgroundI did 1/500 dilution on frozen mouse brain sections(12um). It worked, but with strong background staining.
Date published: 2019-04-02
Rated 4 out of 5 by from Good anti-Reelin abWe used this antibody to confirm the type of cells that we imaged during in-vivo two photon imaging in embryo. It worked quite good on our mouse embryo brain tissue sections 70um.
Date published: 2019-01-22
Rated 4 out of 5 by from It works sosoWe detected the expression of Reelin in epidermal cells, but nothing was detected.
Date published: 2017-09-07
Rated 4 out of 5 by from GoodThis antibody works mouse tissue IF staining, but not for western
Date published: 2017-09-06
Rated 5 out of 5 by from Excellent antibody,I tried this antibody on paraffin sections of human fetal brain and obtained very specific staining
Date published: 2017-05-02
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of Reelin expression in HeLa whole cell lysate.
Date published: 2017-01-19
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-03-12
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Reelin抗体 (E-5) is rated 4.4 out of 5 by 8.
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