Date published: 2025-9-7

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RCK 抗体 (G-12): sc-166489

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说明书
  • RCK抗体(G-12)是小鼠单克隆IgG2b (kappa轻链),规格为200 µg/ml
  • 免疫原氨基酸序列61-170位于human物种的RCK的N-terminus附近
  • 推荐用于 mouse, rat 和 human 来源的RCK WB, IP, IF 和 ELISA检测
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是RCK Antibody (G-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与RCK Antibody (G-12) 打包提供(请参阅下面的订购信息)。

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    RCK,又称DDX6和P54,属于DEAD-box RNA解旋酶家族蛋白,它们有共同的蛋白基序。RCK存在于大多数组织中,是一种解旋酶,具有依赖ATP的RNA解旋活性,按 5'-3'方向降解RNA。在非恶性细胞中,RCK与所有正常的RNA代谢过程相关,包括剪接、输出和翻译起始。编码RCK的基因发生突变可导致该蛋白过表达,从而改变其功能,使其成为癌基因,上调涉及细胞生长和增殖的基因表达。人们认为,RCK通过解旋酶活性来下调mRNA表达,并维持细胞内正常的转录水平。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    RCK 抗体 (G-12) 参考文献:

    1. 人类 DEAD-box 蛋白 rck/p54 的结构洞察其底物识别与构象变化。  |  Matsui, T., et al. 2006. Genes Cells. 11: 439-52. PMID: 16611246
    2. 胞质 RCK 结构域的动态寡聚转换介导了 MthK 钾通道的活性。  |  Kuo, MM., et al. 2007. Proc Natl Acad Sci U S A. 104: 2151-6. PMID: 17287352
    3. RCK 结构域对人类 BK 通道异构激活的贡献。  |  Savalli, N., et al. 2012. J Biol Chem. 287: 21741-50. PMID: 22556415
    4. 与 Ba(2+) 结合的门控环晶体结构揭示了 RCK 结构域激活的基本步骤。  |  Smith, FJ., et al. 2012. Structure. 20: 2038-47. PMID: 23085076
    5. 钾转运蛋白 KtrA K+ 传导调节器(RCK)C 域与环状单磷酸二腺苷(c-di-AMP)复合物的结构研究。  |  Kim, H., et al. 2015. J Biol Chem. 290: 16393-402. PMID: 25957408
    6. 一种保守的 TOR 依赖性 RCK 介导的 mRNA 降解机制可调节自噬。  |  Hu, G., et al. 2015. Nat Cell Biol. 17: 930-942. PMID: 26098573
    7. 确定表皮生长因子受体是沙门氏菌 Rck 依赖性侵袭的受体。  |  Wiedemann, A., et al. 2016. FASEB J. 30: 4180-4191. PMID: 27609774
    8. 具有 RCK 门控环的 Ca2+ 调节 Ca2+ 通道控制植物的共生结合。  |  Kim, S., et al. 2019. Nat Commun. 10: 3703. PMID: 31420535
    9. MthK K+ 通道 RCK 结构域中盎格鲁尺度构象变化的能量学。  |  Lewis, JH. and Lu, Z. 2019. Nat Struct Mol Biol. 26: 808-815. PMID: 31488910
    10. rck/p54 候选原癌基因产物是一种 54 千道尔顿的 D-E-A-D 盒蛋白,在人类和小鼠组织中表达不同。  |  Akao, Y., et al. 1995. Cancer Res. 55: 3444-9. PMID: 7614484

    订购信息

    产品名称产品编号规格价格数量收藏夹

    RCK 抗体 (G-12)

    sc-166489
    200 µg/ml
    $316.00

    RCK (G-12): m-IgGκ BP-HRP 套装

    sc-534778
    200 µg Ab; 40 µg BP
    $354.00

    RCK (G-12): m-IgG2b BP-HRP 套装

    sc-549295
    200 µg Ab; 10 µg BP
    $354.00

    For Western Blot, is it recommended to use denatured or non-denatured conditions with RCK (G-12): sc-166489 monoclonal antibody?

    Asked by: cjMara
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Produced positive Western blot data of RCKProduced positive Western blot data of RCK expression in non-transfected and human RCK transfected 293T whole cell lysates. -SCBT QC
    Date published: 2014-04-24
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    RCK 抗体 (G-12) is rated 5.0 out of 5 by 1.
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