Date published: 2025-9-7

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RCK 抗体 (E-8): sc-166590

5.0(3)
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说明书
  • RCK抗体(E-8)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列61-170位于human物种的RCK的N-terminus附近
  • 推荐用于WB, IP, IF, IHC(P)和ELISA检测mouse, rat, human和avian来源的RCK; 也和以下物种反应,包括: 和 equine, canine, bovine and avian
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 RCK 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RCK 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

RCK,又称DDX6和P54,属于DEAD-box RNA解旋酶家族蛋白,它们有共同的蛋白基序。RCK存在于大多数组织中,是一种解旋酶,具有依赖ATP的RNA解旋活性,按 5'-3'方向降解RNA。在非恶性细胞中,RCK与所有正常的RNA代谢过程相关,包括剪接、输出和翻译起始。编码RCK的基因发生突变可导致该蛋白过表达,从而改变其功能,使其成为癌基因,上调涉及细胞生长和增殖的基因表达。人们认为,RCK通过解旋酶活性来下调mRNA表达,并维持细胞内正常的转录水平。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RCK 抗体 (E-8) 参考文献:

  1. 丙型肝炎病毒相关慢性肝炎患者肝细胞中 DEAD 盒/RNA 螺旋酶蛋白 rck/p54 的过表达及其与肝细胞癌变的关系。  |  Miyaji, K., et al. 2003. J Viral Hepat. 10: 241-8. PMID: 12823589
  2. K+ 通道 RCK 结构域与带电膜表面的静电相互作用。  |  Ptak, CP., et al. 2005. Biochemistry. 44: 62-71. PMID: 15628846
  3. 在人体细胞中,microRNA 诱导的基因沉默对翻译的抑制需要 RCK/p54。  |  Chu, CY. and Rana, TM. 2006. PLoS Biol. 4: e210. PMID: 16756390
  4. 人类 DEAD-box/RNA 解旋酶 rck/p54 通过影响培养细胞的细胞周期来维持细胞生长。  |  Akao, Y., et al. 2006. Int J Oncol. 29: 41-8. PMID: 16773183
  5. 用 RNAi 方法敲除 RCK/p54 的表达可抑制体外和体内人类结直肠癌细胞的增殖。  |  Lin, F., et al. 2008. Cancer Biol Ther. 7: 1669-76. PMID: 18769115
  6. P 体蛋白 Rck/p54 与受抑制的 mRNA 的多重结合。  |  Ernoult-Lange, M., et al. 2012. RNA. 18: 1702-15. PMID: 22836354
  7. 通过 X 射线晶体学分析 MthK RCK 结构域中的 Ca(2+) 结合位点。  |  Smith, FJ. and Rothberg, BS. 2013. Methods Mol Biol. 998: 277-87. PMID: 23529438
  8. K+ 通道 RCK 结构域中 Ca2+ 结合位点之间异构相互作用的结构基础  |  Smith, FJ., et al. 2013. Nat Commun. 4: 2621. PMID: 24126388
  9. 调控枯草芽孢杆菌 Ktr 通道的第二个 RCK 结构域 KtrC 的分子特性。  |  Rocha, R., et al. 2019. J Struct Biol. 205: 34-43. PMID: 30753894
  10. xbx-4是朱伯综合征基因FAM149B1的同源物,通过CCRK和RCK激酶级联调节纤毛形态。  |  Maurya, AK. and Sengupta, P. 2021. Curr Biol. 31: 5642-5649.e5. PMID: 34731674

订购信息

产品名称产品编号规格价格数量收藏夹

RCK 抗体 (E-8)

sc-166590
200 µg/ml
RMB2377.00

RCK (E-8): m-IgG Fc BP-HRP 套装

sc-540127
200 µg Ab; 10 µg BP
RMB2662.00

RCK (E-8): m-IgG1 BP-HRP 套装

sc-541920
200 µg Ab; 20 µg BP
RMB2662.00

If I sent you a set of proteins from other species, would you be able to tell me whether RCK (E-8): sc-166590 should or could work? Thank you

Asked by: Gunnar
Thank you for your question. Since we have not epitope mapped this antibody, what we could do is check the homology against the other species and see if the homology is great enough to think it would work. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com and we'd be glad to help you look into the potential homology.
Answered by: Tech Service
Date published: 2017-06-05

I am using RCK (E-8): sc-166590 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from 抗体效果用的是santa cruz的RCK抗体,条带很清晰,在做的蛋白里面表达还是很高的,有机会还会来用别的产品的。
Date published: 2017-03-24
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by from Good Western Blot data of RCK expression in nonGood Western Blot data of RCK expression in non-transfected and human RCK transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-03-29
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RCK 抗体 (E-8) is rated 5.0 out of 5 by 3.
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