Date published: 2025-9-6
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Rb Antibody (Rb1) - Western Blotting - Image 401701
Rb 抗体 (Rb1) HRP: sc-73598 HRP. 直接 Western 印迹分析 HEL 92. 1. 7 (A), SK-MEL-28 (B), Jurkat (C), MOLT-4 (D) 和 Ramos (E) 全细胞裂解液中 Rb 的表达.
Rb 抗体 (Rb1) HRP: sc-73598 HRP. 直接 Western 印迹分析 HEL 92. 1. 7 (A), SK-MEL-28 (B), Jurkat (C), MOLT-4 (D) 和 Ramos (E) 全细胞裂解液中 Rb 的表达.

Rb 抗体 (Rb1): sc-73598

4.8(4)
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说明书
  • Rb抗体(Rb1)是小鼠单克隆IgG1 κ, 在25篇文献中引用，规格为200 µg/ml
  • 针对视网膜母细胞瘤基因产物β半乳糖苷酶融合蛋白
  • 推荐用于 mouse, rat 和 human 来源的Rb p110 WB, IP, IF, IHC(P) 和 FCM检测
  • 还可偶联琼脂糖， 用于 IP；偶联HRP，用于WB, IHC(P) 和 ELISA; 偶联FITC以及荧光素，用于IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488 和 Alexa Fluor® 647，用于IF, IHC(P) 和 FCM
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取Rb (Rb1): sc-73598的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Rb 抗体 (Rb1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Rb 抗体 (Rb1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Rb抗体（Rb1）是一种小鼠单克隆IgG1 kappa轻链抗体，针对全长人类视网膜母细胞瘤β-半乳糖苷酶融合蛋白（一种参与细胞周期调节的关键肿瘤抑制剂）产生。Rb小鼠单克隆抗体（Rb1）识别Rb p110免疫原序列内的表位，并表现出广泛的物种交叉反应性，可通过蛋白质印迹（WB）、免疫沉淀（IP）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测小鼠、大鼠和人类样本中的Rb。抗Rb单克隆抗体（Rb1）有结合和非结合两种形式。视网膜母细胞瘤蛋白通过与E2F转录因子的复杂相互作用，对细胞周期进展（尤其是G1/S转换）进行精密调节，从而控制分化、凋亡和细胞增殖。在低磷酸化状态下，Rb与E2F因子形成抑制复合物，通过两种主要机制抑制细胞周期相关基因：招募组蛋白去乙酰化酶和SWI/SNF染色质重塑复合物，促进染色质浓缩。这种抑制作用通过细胞周期蛋白依赖性激酶（D1、D2、D3）在多个位点（包括Ser249/Thr252、Thr373、Thr356、Ser780、Ser795、Ser807/Ser811和Thr821/Thr826）的磷酸化作用进行精确调节。这些改变使Rb进入非活动状态，释放E2F因子以激活S期基因。除了直接转录调控外，Rb还与组蛋白甲基转移酶和Polycomb组蛋白结合，从而扩大Rb对染色质结构和基因表达的影响。编码Rb的RB1基因突变可导致小儿视网膜母细胞瘤，并通过破坏这些调节机制导致各种人类肿瘤。通过这些不同的细胞功能，Rb (Rb1)抗体成为研究细胞周期控制、肿瘤抑制途径和癌症发展分子机制的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Rb 抗体 (Rb1)

sc-73598
200 µg/ml
RMB2377.00

Rb (Rb1): m-IgG Fc BP-HRP 套装

sc-527007
200 µg Ab; 10 µg BP
RMB2662.00

Rb (Rb1): m-IgG1 BP-HRP 套装

sc-532380
200 µg Ab; 20 µg BP
RMB2662.00

Rb 抗体 (Rb1) AC

sc-73598 AC
500 µg/ml, 25% agarose
RMB3129.00

Rb 抗体 (Rb1) HRP

sc-73598 HRP
200 µg/ml
RMB2377.00

Rb 抗体 (Rb1) FITC

sc-73598 FITC
200 µg/ml
RMB2482.00

Rb 抗体 (Rb1) PE

sc-73598 PE
200 µg/ml
RMB2580.00

Rb 抗体 (Rb1) Alexa Fluor® 488

sc-73598 AF488
200 µg/ml
RMB2685.00

Rb 抗体 (Rb1) Alexa Fluor® 647

sc-73598 AF647
200 µg/ml
RMB2685.00

Rb 抗体 (Rb1) TRITC

sc-73598 TRITC
200 µg/ml
RMB2407.00

Rb 抗体 (Rb1) Alexa Fluor® 405

sc-73598 AF405
200 µg/ml
RMB2587.00

Can the Anticorpo Rb (sc-73598) identify both the hypophosphorylated and hyperphosphorylated form of Rb? Are there any molecular weight differences? 

Asked by: Beatrice
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

is this RB1 antibody similar to BD BIOSCIENCE g3 245 used to test loss of expression in large cell neuroendocrine and small cell carcinoma lung ?

Asked by: marguerite
Thank you for your question. This is a different clone than “G3-245”. Please contact our Technical Service Department for further support.
Answered by: Technical Support
Date published: 2019-06-16

How much protein do you recommend loading on the gel for Western blot detection using Rb (Rb1): sc-73598?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 13
Date published: 2017-02-08

Hello dear Please tell me could RB1-sc-73598 is able to detect both hyper and hypo phosphorylation form of RB in western blot analysis?

Asked by: Hamid
Thank you for your question. This mouse monoclonal, Rb Antibody (Rb1): sc-73598, has been verified to detect Total Rb, both phosphorylated and un-phosphorylated Rb protein. We have not performed experiments to evaluate the reactivity of this clone against hyper and hypo phosphorylated forms of the protein. There are currently 8 product citations that we are aware of using this antibody in Western blot. A link to a list of these journal articles is provided below from the product citation search on our website. https://www.scbt.com/scbt/misc/citationResults.jsp?_requestid=237968
Answered by: Technical Support
Date published: 2016-12-16
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Rated 5 out of 5 by from Very popular choice!This monoclonal Rb Antibody (Rb1) is a very popular choice and has been featured in nearly a dozen publications, primarily used for WB and IHC.
Date published: 2017-01-27
Rated 4 out of 5 by from Good antibody for Western Blot against KGood antibody for Western Blot against K-562, A-431, HeLa and SK-LMS-1 whole cell lysates. -SCBT QC
Date published: 2015-02-14
Rated 5 out of 5 by from Works great for human Rb transfected 293TWorks great for human Rb transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by from Excellent intracellular FCM data of fixedExcellent intracellular FCM data of fixed and permeabilized Jurkat cells. -SCBT QC
Date published: 2014-11-28
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Rb 抗体 (Rb1) is rated 4.8 out of 5 by 4.
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