Date published: 2025-9-6
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Rb Antibody (IF8) - Western Blotting - Image 390155
Rb 抗体 (IF8) Alexa Fluor® 488: sc-102 AF488. Ramos (A), MDA-MB-231 (B) 和 MOLT-4 (C) 全细胞裂解液中 Rb 表达的荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.
Rb 抗体 (IF8) Alexa Fluor® 488: sc-102 AF488. Ramos (A), MDA-MB-231 (B) 和 MOLT-4 (C) 全细胞裂解液中 Rb 表达的荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.

Rb 抗体 (IF8): sc-102

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说明书
  • Rb 抗体 IF8 是小鼠单克隆 IgG1 κ，Rb抗体, 在388篇文献中引用，规格为200 µg/ml
  • 免疫成视网膜细胞瘤基因产物/β-galactosidase融合蛋白
  • Rb 抗体 (IF8) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Rb p110
  • 抗Rb抗体（IF8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取Rb (IF8): sc-102的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Rb 抗体 (IF8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Rb 抗体 (IF8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Rb抗体（IF8）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光、石蜡包埋切片免疫组织化学和流式细胞术检测小鼠、大鼠和人源Rb蛋白（也称为视网膜母细胞瘤（RB）转录共抑制因子1或RB1）。Rb单克隆抗体（IF8）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合型。小儿癌症视网膜母细胞瘤和其他人类肿瘤的形成可归因于视网膜母细胞瘤肿瘤抑制基因的突变。Rb蛋白通过协调G1/S期细胞周期与转录机制（包括异二聚体E2F家族）来调节分化、细胞凋亡和细胞周期控制。在G1期，依赖细胞周期蛋白D（D1、D2、D3）的激酶介导的Rb在Ser 795位点的磷酸化标志着Rb从转录抑制的低磷酸化状态转变为非活性磷酸化状态，这种状态可能通过有丝分裂中多达16个假定的丝氨酸或苏氨酸残基（包括Ser 249/Thr 252、Thr 373、Thr 356、Ser 780、Ser 807/Thr 807）的差异磷酸化得以维持 多达16个假定的丝氨酸或苏氨酸残基，包括Ser 249/Thr 252、Thr 373、Thr 356、Ser 780、Ser 807/Ser 811和Thr 821/Thr 826。低磷酸化的Rb通过直接蛋白质-蛋白质相互作用抑制控制细胞周期的基因转录，通过结合和灭活转录因子的启动子，以及通过招募组蛋白去乙酰化酶，后者去乙酰化启动子区域并增强核小体形成，从而掩盖转录增强顺式元件。建议研究细胞周期调控和癌症发展分子机制的研究人员使用抗Rb单克隆抗体（IF8）。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rb 抗体 (IF8) 参考文献:

  1. 发现了一种调控基调，它能控制特定上游细胞周期蛋白依赖性激酶位点的暴露，这些位点决定了 pRb 的构象和生长抑制活性。  |  Driscoll, B., et al. 1999. J Biol Chem. 274: 9463-71. PMID: 10092628
  2. 视网膜母细胞瘤蛋白磷酸化位点的谷氨酸突变对功能有多种影响。  |  Barrientes, S., et al. 2000. Oncogene. 19: 562-70. PMID: 10698526
  3. 转化生长因子β抑制pRB在多个丝氨酸/苏氨酸位点的磷酸化，并对人类髓系白血病细胞中pRB家族-E2F复合物的形成进行不同程度的调控。  |  Hu, X., et al. 2000. Biochem Biophys Res Commun. 276: 930-9. PMID: 11027571
  4. 视网膜母细胞瘤蛋白（Rb）在BAG-1核定域中的作用：对结直肠肿瘤细胞存活的影响。  |  Clemo, NK., et al. 2005. Biochem Soc Trans. 33: 676-8. PMID: 16042572
  5. 视网膜母细胞瘤蛋白（RB）与E2F3相互作用，控制支持细胞（Sertoli cells）的最终分化。  |  Rotgers, E., et al. 2014. Cell Death Dis. 5: e1274. PMID: 24901045
  6. 视网膜母细胞瘤蛋白（Rb）丝氨酸-807 的磷酸化是与 Bax 结合所必需的。  |  Antonucci, LA., et al. 2014. Cell Cycle. 13: 3611-7. PMID: 25483096
  7. 视网膜母细胞瘤蛋白是一种内在的 BRD4 抑制剂，可调节癌症中小分子 BET 抑制剂的敏感性。  |  Ding, D., et al. 2022. Nat Commun. 13: 6311. PMID: 36274096
  8. 视网膜母细胞瘤蛋白与细胞周期控制  |  Weinberg, RA. 1995. Cell. 81: 323-30. PMID: 7736585
  9. pRB 对转录的直接抑制以及特定细胞周期蛋白对其的逆转。  |  Bremner, R., et al. 1995. Mol Cell Biol. 15: 3256-65. PMID: 7760821
  10. 癌细胞周期  |  Sherr, CJ. 1996. Science. 274: 1672-7. PMID: 8939849
  11. 细胞周期蛋白 D1/Cdk4 通过特定位点磷酸化调节视网膜母细胞瘤蛋白介导的细胞周期停滞。  |  Connell-Crowley, L., et al. 1997. Mol Biol Cell. 8: 287-301. PMID: 9190208
  12. Rb 与组蛋白去乙酰化酶相互作用，抑制转录。  |  Luo, RX., et al. 1998. Cell. 92: 463-73. PMID: 9491888

订购信息

产品名称产品编号规格价格数量收藏夹

Rb 抗体 (IF8)

sc-102
200 µg/ml
RMB2377.00

Rb (IF8): m-IgG Fc BP-HRP 套装

sc-528097
200 µg Ab; 10 µg BP
RMB2662.00

Rb (IF8): m-IgGκ BP-HRP 套装

sc-520395
200 µg Ab, 40 µg BP
RMB2662.00

Rb (IF8): m-IgG1 BP-HRP 套装

sc-542756
200 µg Ab; 20 µg BP
RMB2662.00

Rb 抗体 (IF8) AC

sc-102 AC
500 µg/ml, 25% agarose
RMB3129.00

Rb 抗体 (IF8) HRP

sc-102 HRP
200 µg/ml
RMB2377.00

Rb 抗体 (IF8) FITC

sc-102 FITC
200 µg/ml
RMB2482.00

Rb 抗体 (IF8) PE

sc-102 PE
200 µg/ml
RMB2580.00

Rb 抗体 (IF8) Alexa Fluor® 488

sc-102 AF488
200 µg/ml
RMB2685.00

Rb 抗体 (IF8) Alexa Fluor® 546

sc-102 AF546
200 µg/ml
RMB2685.00

Rb 抗体 (IF8) Alexa Fluor® 594

sc-102 AF594
200 µg/ml
RMB2685.00

Rb 抗体 (IF8) Alexa Fluor® 647

sc-102 AF647
200 µg/ml
RMB2685.00

Rb 抗体 (IF8) Alexa Fluor® 680

sc-102 AF680
200 µg/ml
RMB2685.00

Rb 抗体 (IF8) Alexa Fluor® 790

sc-102 AF790
200 µg/ml
RMB2685.00

Rb 抗体 (IF8) TRITC

sc-102 TRITC
200 µg/ml
RMB2407.00

Rb 抗体 (IF8) Alexa Fluor® 405

sc-102 AF405
200 µg/ml
RMB2685.00

Hi, Is this a polyclonal; or monoclonal antibody? Which site of RB this antibody is recognizing?

Asked by: Pooja
Thank you for your question. sc-102 Rb (IF8) is a monoclonal antibody. We are unable to provide a an epitope range. For further assistance please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-04-04

Do you happen to know whether this mAb (IF8) is reactive with pig (porcine) RB by IHC and WB, looking for a pig RB antibody? Thanks!

Asked by: JWS1
Thank you for your question. Rb Antibody (IF8): sc-102 is recommended for detection of Rb p110 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. We have no internal data for this antibody's reactivity in porcine tissues and it is not recommended for this use. However there is a publication in which this antibody was used for detection of Rb in pig. The corresponding PMID is 16293970.
Answered by: Tech Support Europe
Date published: 2019-07-11

Hello My name is Laura, I bought the antibody sc-102 Rb and I am getting two bands, I need to know... ¿Is it a phosphorylated form of Rb or a nonspecific band? thank you so much

Asked by: Lauralopezbanuelos
Thank you for your question. We would recommend treating your lysates with Lambda Phosphatase: sc-200312A to confirm whether or not the antibody is specifically detecting phosphorylated Rb in your samples.
Answered by: Technical Support
Date published: 2019-01-26

I'm interested in sc-102 and would like to receive a FREE 10 µg sample of Rb (IF8): sc-102 for testing. Is this possible ?

Asked by: Janine Kruit
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Is the epitope that is recognised by the antibody known? (which aa region?) 

Asked by: ResearcherfromLeuven
Thank you for your inquiry. The epitope region is not known. For additional questions, please contact our Technical Service by email ([email protected]) or phone (00800 4573 8000).
Answered by: Technical Support Europe
Date published: 2017-12-20

Does this antibody (SC_102) bind with other proteins in Rb family? I can detect three bands of which their M.W. are within range of protein marker from 100 to 150 kD. Are all these bands Rb protein? (protein lysate from MCF7, 293T; Ab dilution 1:800)

Asked by: Wareerat
Thank you for your question. This mouse monoclonal antibody, Rb (IF8): sc-102, is specific for the detection of Rb. It sounds like your results are inconsistent with our Western blot results published on our website for this antibody. We would recommend you include a secondary antibody control (omit primary antibody incubation) to confirm if any of the other bands you are seeing are due to non-specific binding of the secondary antibody alone. Please contact our Technical Service Department for further help in troubleshooting your Western blot results with this antibody. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-11-15

Does this "Rb Antibody (IF8)" bind with both the phosphorylated and the un-phosphorylated Rb proteins? I can then detect the relative number of the phosphorylated Rb with one of the p-Rb antibodies, is this correct? Thanks.

Asked by: blcxfk
Thank you for your question. This mouse monoclonal antibody, Rb (IF8): sc-102, is not phospho-specific and therefore should be able to detect both the un-phosphorylated and phosphorylated Rb protein. If you use this antibody as a control along with a phospho-specific Rb antibody you will observe a qualitative but not a quantitative difference by Western blot.
Answered by: Technical Support
Date published: 2022-09-04

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 4 out of 5 by from works well for WBI used this antibody for WB and obtained good results.
Date published: 2020-08-21
Rated 5 out of 5 by from To recommendMonoclonal IF8 was obtained from S. Cruz as complimentary antibody. It was used at dilution 1/200 to probe whole cell extracts (50µg) from nocodazole-synchronized U2OS resolved on 7.5% SDS-PAGE Anderson gel system. M: mitosis; G1: G1 phase.
Date published: 2019-04-10
Rated 5 out of 5 by from good productThis antibody works well for western blot experiment.
Date published: 2018-08-22
Rated 5 out of 5 by from Great antibody!Specific and low background. I see two bands close to each other. they might be phosphorylated and un-phosphoyrlated forms.
Date published: 2018-03-01
Rated 5 out of 5 by from Works well for WBworks very well for western - samples were human cells in culture
Date published: 2017-11-21
Rated 3 out of 5 by from Good antibody for western blot to dilute 250x?The batch of antibody I have worked well on HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific but very weak. The antibody was diluted 500 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2017-11-16
Rated 5 out of 5 by from GreatWorks well at 1:250 in LNCaP cell lysate. Detects approximately 110 kDa in 8% PAGE
Date published: 2017-09-21
Rated 5 out of 5 by from Great for western blotsGives clean band at the expected size diluted 1:500 in milk
Date published: 2017-09-20
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Rb 抗体 (IF8) is rated 4.7 out of 5 by 27.
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