Date published: 2025-9-6
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Rb Antibody (C-2) - Western Blotting - Image 373404
Rb 抗体 (C-2): sc-74562. A-431 (A), Y79 (B) 和 RAW 264. 7 (C) 全细胞裂解液中 Rb 表达的 Western 印迹分析.
Rb 抗体 (C-2): sc-74562. A-431 (A), Y79 (B) 和 RAW 264. 7 (C) 全细胞裂解液中 Rb 表达的 Western 印迹分析.

Rb 抗体 (C-2): sc-74562

4.9(7)
寫評論提問

说明书
  • Rb抗体(C-2)是小鼠单克隆IgG2a κ, 在38篇文献中引用，规格为200 µg/ml
  • 针对氨基酸769-921，在Rb的mouse起源的C-terminus处进行映射
  • 抗-Rb 抗体 (C-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Rb
  • 抗Rb抗体（C-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rb (C-2): sc-74562的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是Rb Antibody (C-2) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Rb Antibody (C-2) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Rb抗体（C-2）是一种IgG2aκ小鼠单克隆Rb抗体（也称为Rb抗体或RB1抗体），通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Rb。Rb抗体（C-2）可作为非偶联的抗-Rb抗体，以及多种偶联形式的抗Rb抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。儿童癌症视网膜母细胞瘤和其他人类肿瘤的形成可归因于视网膜母细胞癌抑癌基因的突变。视网膜母细胞瘤肿瘤抑制基因产物，称为Rb或pRb，通过在G1/S期协调细胞周期与包括异二聚体E2F家族在内的转录机制来调节分化、凋亡和细胞周期控制。在G1期，细胞周期蛋白D（D1、D2、D3）依赖性激酶介导的Rb在Ser 795的磷酸化标志着Rb从转录抑制的低磷酸化状态转变为无活性的磷酸化状态，这可以通过多达16个推定的丝氨酸或苏氨酸残基的差异磷酸化通过有丝分裂来维持，包括Ser 249/Thr 252、Thr 373、Thr 356、Ser 780、Ser 807/Ser 811和Thr 821/Thr 826。低磷酸化的Rb通过直接的蛋白质-蛋白质相互作用，通过结合和失活转录因子的启动子，以及通过招募组蛋白脱乙酰酶来抑制控制细胞周期的基因的转录，组蛋白脱乙酰酶使启动子区域脱乙酰并增强核小体的形成，从而掩盖转录增强的顺式元件。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Rb 抗体 (C-2)

sc-74562
200 µg/ml
RMB2377.00

Rb (C-2): m-IgG Fc BP-HRP 套装

sc-525730
200 µg Ab; 10 µg BP
RMB2662.00

Rb 抗体 (C-2) AC

sc-74562 AC
500 µg/ml, 25% agarose
RMB3129.00

Rb 抗体 (C-2) HRP

sc-74562 HRP
200 µg/ml
RMB2377.00

Rb 抗体 (C-2) FITC

sc-74562 FITC
200 µg/ml
RMB2482.00

Rb 抗体 (C-2) PE

sc-74562 PE
200 µg/ml
RMB2580.00

Rb 抗体 (C-2) Alexa Fluor® 488

sc-74562 AF488
200 µg/ml
RMB2685.00

Rb 抗体 (C-2) Alexa Fluor® 546

sc-74562 AF546
200 µg/ml
RMB2685.00

Rb 抗体 (C-2) Alexa Fluor® 594

sc-74562 AF594
200 µg/ml
RMB2685.00

Rb 抗体 (C-2) Alexa Fluor® 647

sc-74562 AF647
200 µg/ml
RMB2685.00

Rb 抗体 (C-2) Alexa Fluor® 680

sc-74562 AF680
200 µg/ml
RMB2685.00

Rb 抗体 (C-2) Alexa Fluor® 790

sc-74562 AF790
200 µg/ml
RMB2685.00

Why can't I get the band in A549 cells? I diluted it 200 times by using BSA.

Asked by: Chirs
Thank you for your question. We do not have any data for Rb antigen in A549 cells. We would recommend testing a different cell line as a postive control for Rb, such as A-431 Whole Cell Lysate: sc-2201. We recommend loading 50 ug protein. And we also recommend using 5% nonfat milk in TBST for blocking and incubating both primary and secondary antibodies.
Answered by: Technical Support
Date published: 2019-02-06

Can you recommend a positive control to use with Rb (C-2): sc-74562 monoclonal antibody for Western blot?

Asked by: cjMara
We recommend using SW480: sc-2155, NIH/3T3: sc-2138 or KNRK: sc-2141 nuclear extracts to use as positive controls as we have great Western blot data showing specific detection of Rb in these cell lines.
Answered by: Technical Support 10
Date published: 2017-01-20
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Rated 5 out of 5 by from good productThis antibody works well for western blot application.
Date published: 2018-08-22
Rated 5 out of 5 by from strong bandsClear Western data. There was very little background with the strong bands. Good expression in Molt-4 and Jurkat cells.
Date published: 2017-10-03
Rated 5 out of 5 by from Great western dataVery clear western blot data; strong signal with little to no background
Date published: 2017-02-05
Rated 5 out of 5 by from Very strong antibody!Great choice for Western blot detection of Rb. Solid strong bands, consistent and reliable.
Date published: 2017-01-09
Rated 5 out of 5 by from Strong signal achieved using SW480 wholeStrong signal achieved using SW480 whole cell lysate and NIH/3T3 and KNRK nuclear extracts. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Favorable band in WB against human Rb transfectedFavorable band in WB against human Rb transfected 293T and K-562 whole cell lysates. -SCBT QC
Date published: 2015-01-31
Rated 4 out of 5 by from Good antibody for WB using AGood antibody for WB using A-431and SK-MEL-28 whole cell lysates.
Date published: 2014-12-04
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Rb 抗体 (C-2) is rated 4.9 out of 5 by 7.
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