Date published: 2025-9-7
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RasGRP1 Antibody (199) - Western Blotting - Image 360672
RasGRP1 抗体 (199): sc-8430. 小鼠大脑 (A) 和大鼠大脑 (B) 组织提取物中 RasGRP1 表达的 Western 印迹分析.
RasGRP1 抗体 (199): sc-8430. 小鼠大脑 (A) 和大鼠大脑 (B) 组织提取物中 RasGRP1 表达的 Western 印迹分析.

RasGRP1 抗体 (199): sc-8430

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说明书
  • RasGRP1 抗体 199 是小鼠单克隆 IgG1 κ，RasGRP1抗体, 在27篇文献中引用，规格为200 µg/ml
  • 针对全长RasGRP1，rat起源
  • RasGRP1 抗体 (199) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 RasGRP1
  • 抗RasGRP1抗体（199）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RasGRP1 (199): sc-8430的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是RasGRP1 Antibody (199) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与RasGRP1 Antibody (199) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

附加RasGRP1抗体，包括RasGRP4

RasGRP抗体（199）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测鼠、大鼠和人源的RasGRP。Anti-RasGRP antibody (199) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RasGRP作为Ras的鸟嘌呤核苷酸释放因子在细胞信号传导中起着至关重要的作用，而Ras是MAP激酶通路调节的关键因子。该通路对于包括生长、分化、存活在内的各种细胞过程至关重要。RasGRP包含两个结合钙离子的EF手结构域和一个结合二酰基甘油（DAG）的结构域，使RasGRP能够整合钙离子和DAG的信号，这对Ras的激活非常重要。RasGRP在神经系统和淋巴组织中的表达凸显了其在连接细胞外信号和细胞内反应中的重要性，使RasGRP成为调节免疫反应和神经信号传导的重要成分。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RasGRP1 抗体 (199) 参考文献:

  1. 含SH2和SH3结构域的蛋白质GRB2将受体酪氨酸激酶与ras信号传导联系起来。  |  Lowenstein, EJ., et al. 1992. Cell. 70: 431-42. PMID: 1322798
  2. GRB2 在连接胰岛素受体和 Ras 信号通路方面的功能。  |  Skolnik, EY., et al. 1993. Science. 260: 1953-5. PMID: 8316835
  3. 正常和致癌的 p21ras 蛋白与 c-Raf-1 的氨基末端，N 端调节结构域结合。  |  Zhang, XF., et al. 1993. Nature. 364: 308-13. PMID: 8332187
  4. 人类 Sos1：与 GRB2 结合的 Ras 的鸟嘌呤核苷酸交换因子。  |  Chardin, P., et al. 1993. Science. 260: 1338-43. PMID: 8493579
  5. RasGRP，一种具有钙和二酰甘油结合基团的 Ras 鸟苷酸释放蛋白。  |  Ebinu, JO., et al. 1998. Science. 280: 1082-6. PMID: 9582122
  6. 通过磷酸酯响应性 C1 结构域调节 RasGRP。  |  Tognon, CE., et al. 1998. Mol Cell Biol. 18: 6995-7008. PMID: 9819387

订购信息

产品名称产品编号规格价格数量收藏夹

RasGRP1 抗体 (199)

sc-8430
200 µg/ml
RMB2377.00

RasGRP1 (199): m-IgG Fc BP-HRP 套装

sc-528232
200 µg Ab; 10 µg BP
RMB2662.00

RasGRP1 (199): m-IgGκ BP-HRP 套装

sc-520558
200 µg Ab, 40 µg BP
RMB2662.00

RasGRP1 (199): m-IgG1 BP-HRP 套装

sc-542829
200 µg Ab; 20 µg BP
RMB2662.00

RasGRP1 抗体 (199) AC

sc-8430 AC
500 µg/ml, 25% agarose
RMB3129.00

RasGRP1 抗体 (199) HRP

sc-8430 HRP
200 µg/ml
RMB2377.00

RasGRP1 抗体 (199) FITC

sc-8430 FITC
200 µg/ml
RMB2482.00

RasGRP1 抗体 (199) PE

sc-8430 PE
200 µg/ml
RMB2580.00

RasGRP1 抗体 (199) Alexa Fluor® 488

sc-8430 AF488
200 µg/ml
RMB2685.00

RasGRP1 抗体 (199) Alexa Fluor® 546

sc-8430 AF546
200 µg/ml
RMB2685.00

RasGRP1 抗体 (199) Alexa Fluor® 594

sc-8430 AF594
200 µg/ml
RMB2685.00

RasGRP1 抗体 (199) Alexa Fluor® 647

sc-8430 AF647
200 µg/ml
RMB2685.00

RasGRP1 抗体 (199) Alexa Fluor® 680

sc-8430 AF680
200 µg/ml
RMB2685.00

RasGRP1 抗体 (199) Alexa Fluor® 790

sc-8430 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with RasGRP (199): sc-8430 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from BANDA SINGOLA!Testato questo anticorpo su cellule MCF-7. Preparato 1:500 in latte 5% con incubazione o.n. Funziona molto bene
Date published: 2025-03-19
Rated 5 out of 5 by from Positive western blot results in rat tissueWe used this antibody for expression detection of RasGRP by western blot. We got a positive band around the correct molecular weight in rat tissue.
Date published: 2017-02-07
Rated 5 out of 5 by from Produced great immunofluorescence membraneProduced great immunofluorescence membrane and cytoplasmic staining in methanol-fixed EOC 20 cells. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by from Good WB publishable data gathered with mouseGood WB publishable data gathered with mouse thymic subpopulations - SCBT Publication Review
Date published: 2015-04-23
Rated 5 out of 5 by from Successful application of this antibody inSuccessful application of this antibody in IHCP using human meningiomas -SCBT Publication Review
Date published: 2015-03-21
Rated 5 out of 5 by from Band detected at expected mw in in mouseBand detected at expected mw in in mouse and rat brain extracts. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for IHC and WB on rat transfected keratinocytes - SCBT Publication Review
Date published: 2015-02-21
Rated 5 out of 5 by from Publishable IF data with mouse bone marrowPublishable IF data with mouse bone marrow mononuclear cells (MNCs) - SCBT Publication Review
Date published: 2015-02-02
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RasGRP1 抗体 (199) is rated 4.9 out of 5 by 9.
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