Date published: 2025-9-6
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RARα/Retinoic Acid Receptor α Antibody (C-1) - Western Blotting - Image 375889
RARα 抗体 (C-1): sc-515796. 在 HeLa (A), K-562 (B), HL-60 (C), RAW 264. 7 (D), NIH/3T3 (E) 和 F9 (F) 全细胞裂解液中 RARα 表达的 Western 印迹分析.
RARα 抗体 (C-1): sc-515796. 在 HeLa (A), K-562 (B), HL-60 (C), RAW 264. 7 (D), NIH/3T3 (E) 和 F9 (F) 全细胞裂解液中 RARα 表达的 Western 印迹分析.

RARα/Retinoic Acid Receptor α 抗体 (C-1): sc-515796

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说明书
  • RARα/Retinoic Acid Receptor α 抗体 C-1 是小鼠单克隆 IgG1 κ，RARα/Retinoic Acid Receptor α抗体, 在25篇文献中引用，规格为200 µg/ml
  • 免疫human来源RARα内部区域的63-362氨基酸
  • RARα/Retinoic Acid Receptor α 抗体 (C-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 RARα
  • 抗RARα/Retinoic Acid Receptor α抗体（C-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RARα/Retinoic Acid Receptor α (C-1): sc-515796的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是RARα/Retinoic Acid Receptor α Antibody (C-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与RARα/Retinoic Acid Receptor α Antibody (C-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外RAR抗体，包括RAR α, RAR β, RAR β 2和RAR γ

RARα抗体（C-1）是一种小鼠单克隆IgG1 kappa轻链抗体，专门用于检测视黄酸受体α（RARα），也称为RARA或NR1B1。RARα单克隆抗体（C-1）靶向人类RARα蛋白的63-362氨基酸的内部表位，确保在多种物种（包括小鼠、大鼠和人类）中精确、可靠地结合。RARα是一种重要的核转录因子，位于细胞核内，负责调节维生素A的重要代谢产物--视黄酸的作用，而维生素A对于调节细胞分化、增殖和凋亡相关的基因表达至关重要。RARα的正常功能对于脊椎动物的发育和维持组织稳态至关重要，而RARα失调与多种疾病密切相关，尤其是急性早幼粒细胞白血病（APL）。在急性早幼粒细胞白血病中，染色体易位将RARα基因与PML基因融合，形成致癌的PML/RARα融合蛋白，破坏正常的凋亡途径，阻止髓样分化。RARα (C-1)抗体在蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）中均表现出色。这使得抗RARα抗体（C-1）成为研究RARα信号通路及其在疾病机理中作用的研究人员不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RARα/Retinoic Acid Receptor α 参考文献:

  1. 急性早幼粒细胞白血病治疗的当前问题。  |  Slack, JL. and Rusiniak, ME. 2000. Ann Hematol. 79: 227-38. PMID: 10870476
  2. 维甲酸可诱导由融合了维甲酸受体α基因的早幼粒细胞白血病基因编码的致白血病蛋白降解。  |  Wolf, G. and Smas, CM. 2000. Nutr Rev. 58: 211-4. PMID: 10941258
  3. 全反式维甲酸诱导急性早幼粒细胞白血病细胞分化的机制。  |  Zhang, JW., et al. 2000. J Biosci. 25: 275-84. PMID: 11022230
  4. 急性早幼粒细胞白血病（AML-M3）--第 2 部分：分子缺陷, DNA 诊断以及白血病发生和分化治疗的拟议模型。  |  Randolph, TR. 2000. Clin Lab Sci. 13: 106-16. PMID: 11066449
  5. 视黄醇对脂质和脂蛋白代谢的调节。  |  Staels, B. 2001. J Am Acad Dermatol. 45: S158-67. PMID: 11606948
  6. 果蝇 EcR 基因编码一种蜕皮激素受体，它是类固醇受体超家族的一个新成员。  |  Koelle, MR., et al. 1991. Cell. 67: 59-77. PMID: 1913820
  7. 视黄酸受体-α调节人体血小板的合成过程  |  Schwertz, H., et al. 2017. J Thromb Haemost. 15: 2408-2418. PMID: 28981191
  8. 视黄酸受体α和CRABP2在转移性肾母细胞瘤中的组织表达  |  Percicote, AP., et al. 2018. Diagn Pathol. 13: 9. PMID: 29378601
  9. 选择性维甲酸受体α拮抗剂ER-50891及相关类似物用于男性避孕的研究  |  Kyzer, JL., et al. 2023. Arch Pharm (Weinheim). 356: e2300031. PMID: 37154197
  10. 磷酸化增强了甲状腺激素受体与视黄醇 X 受体的靶基因序列依赖性二聚化。  |  Bhat, MK., et al. 1994. Proc Natl Acad Sci U S A. 91: 7927-31. PMID: 8058736
  11. [视黄酸对发育的基因控制]。  |  Mark, M., et al. 1997. C R Seances Soc Biol Fil. 191: 77-90. PMID: 9181129
  12. 维甲酸及其核受体在上消化道癌症的发展和预防中的作用。  |  Lotan, R. 1997. Environ Health Perspect. 105 Suppl 4: 985-8. PMID: 9255592

订购信息

产品名称产品编号规格价格数量收藏夹

RARα/Retinoic Acid Receptor α 抗体 (C-1)

sc-515796
200 µg/ml
RMB2377.00

RARα/Retinoic Acid Receptor α (C-1): m-IgG Fc BP-HRP 套装

sc-531516
200 µg Ab; 10 µg BP
RMB2662.00

RARα/Retinoic Acid Receptor α (C-1): m-IgGκ BP-HRP 套装

sc-525221
200 µg Ab, 40 µg BP
RMB2662.00

RARα/Retinoic Acid Receptor α (C-1): m-IgG1 BP-HRP 套装

sc-544602
200 µg Ab; 20 µg BP
RMB2662.00

RARα/Retinoic Acid Receptor α 抗体 (C-1) AC

sc-515796 AC
500 µg/ml, 25% agarose
RMB3129.00

RARα/Retinoic Acid Receptor α 抗体 (C-1) HRP

sc-515796 HRP
200 µg/ml
RMB2377.00

RARα/Retinoic Acid Receptor α 抗体 (C-1) FITC

sc-515796 FITC
200 µg/ml
RMB2482.00

RARα/Retinoic Acid Receptor α 抗体 (C-1) PE

sc-515796 PE
200 µg/ml
RMB2580.00

RARα/Retinoic Acid Receptor α 抗体 (C-1) Alexa Fluor® 488

sc-515796 AF488
200 µg/ml
RMB2685.00

RARα/Retinoic Acid Receptor α 抗体 (C-1) Alexa Fluor® 546

sc-515796 AF546
200 µg/ml
RMB2685.00

RARα/Retinoic Acid Receptor α 抗体 (C-1) Alexa Fluor® 594

sc-515796 AF594
200 µg/ml
RMB2685.00

RARα/Retinoic Acid Receptor α 抗体 (C-1) Alexa Fluor® 647

sc-515796 AF647
200 µg/ml
RMB2685.00

RARα/Retinoic Acid Receptor α 抗体 (C-1) Alexa Fluor® 680

sc-515796 AF680
200 µg/ml
RMB2685.00

RARα/Retinoic Acid Receptor α 抗体 (C-1) Alexa Fluor® 790

sc-515796 AF790
200 µg/ml
RMB2685.00

Does this antibody work for Chromatin Immunoprecipitation?

Asked by: Sharmistha
Thank you for your question. RARα/Retinoic Acid Receptor α Antibody (C-1) is recommended for detection of RARα of mouse, rat and human origin by WB, IP, IF and ELISA. For ChIP, we can offer this antibody in custom formulation with 10x concentration and no carrier protein. Please contact technical service for details.
Answered by: Tech Support Europe
Date published: 2025-03-07

RARa antibody(C-1) also can recognize RARa when bound to PML in the PML-RARa complex? RARa antibody(C-20) would be better (always for WB)?

Asked by: roberta
Thank you for your inquiry. We do not have any results showing whether this complex would cause steric hindrance preventing binding of the antibody. We cannot say whether or not the target would be efficiently recognized. RARα/Retinoic Acid Receptor α Antibody (C-1): sc-515796 is available in sample size, so you could get in touch with our technical service team to arrange for a sample of the antibody.
Answered by: Tech Support Europe
Date published: 2024-05-10

I used this on mice liver tissue total proteins western blot but I get two bands around 52 and another band around 37. I would like to know if there is anything to fix it?

Asked by: fathi21
Thank you for your question. The expected molecular weight for this protein is ~52kDa, but we do not have any information regarding a lower sized band. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2019-05-20

I would like to use this antibody for IHC(p) using DAB. May you recommend a secundary antibody?

Asked by: cristiane pereira
Thank you for your question. This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections and so IHC(P) is not a recommended application at this time. RARα Antibody (C-1) is a mouse monoclonal IgG1. For IHC(P), we recommend using a secondary antibody that is anti-mouse IgG or anti-mouse IgG1. For immunoperoxidase staining with DAB as the substrate, we would recommend using either our Mouse IgG Binding Protein conjugated to HRP, m-IgGκ BP-HRP: sc-516102, or our Mouse IgG Binding Protein conjugated to Biotin, m-IgGκ BP-B: sc-516142 in combination with our ImmunoCruz® ABC Kit: sc-516216 for signal amplification.
Answered by: Technical Support
Date published: 2019-04-25

Can this antibody be used for ChiP assays in mouse? Is there a reference for the same? Do I need to order a more concentrated form of the antibody for that purpose?

Asked by: Namr
Thank you for your question. This antibody has not yet been tested in a ChIP application, and there are no references that we are aware of at this time for ChIP.
Answered by: Technical Support
Date published: 2019-04-20

Can RARα Antibody ( (C-1): sc-515796) be used in  immunohistochemistry staining of human tissue 

Asked by: xueju
Thank you for your question. We have not yet tested this mouse monoclonal antibody, RARα (C-1): sc-515796 on formalin-fixed paraffin-embedded tissues and so this application is not recommended at this time. We do warranty the antibody for use in IF, or immunofluorescent staining of fresh or frozen tissues or cells fixed in acetone or methanol. However, we do not yet have any data in this application to show on our website. As soon as IHC or IF data is available, we will publish it on our website. We do currently offer one other antibody to this target, RARα (1C10): sc-293417. However, this antibody has also not yet been tested for immunohistochemistry. For a limited time, we are offering a free 10µg sample of RARα (C-1): sc-515796. If you would like to request a sample for testing in your application, please contact our Technical Service Department. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-05-15

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if RARα (C-1): sc-515796 mouse monoclonal antibody will work?

Asked by: Trav11
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-03-01

RARa antibody(C-1) also can recognize RAR beta or RAR gamma?

Asked by: rere
Thank you for your question. RARα Antibody (C-1): sc-515796 is a mouse monoclonal antibody that is specific for RARα of mouse, rat and human origin. It is not expected to cross-react with RARβ or RARγ.
Answered by: Technical Support
Date published: 2017-01-11
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Rated 1 out of 5 by from no da una banda claraDa demasiado background, es dificil interpretar el resultado
Date published: 2024-10-28
Rated 5 out of 5 by from Good antibody for WBWe used it at 1:100 diluition and it works very well. We are satisfied.
Date published: 2023-09-14
Rated 5 out of 5 by from Works for ChIPI bought this antibody to try because another RARa antibody I got from another supplier had a very low signal. This RARa antibody had a much higher signal and was more specific.
Date published: 2022-11-06
Rated 5 out of 5 by from Dr SadekAb work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Date published: 2018-11-21
Rated 5 out of 5 by from Excellent antibodyThis antibody works very well with minimal background.
Date published: 2018-05-02
Rated 4 out of 5 by from 反応性がよい。一次抗体は1000倍に希釈して用いましたが、反応性がよく、しっかりとしたバンドが特異的に検出されましたので引き続き使用したいと考えております。
Date published: 2018-02-02
Rated 5 out of 5 by from Quite good for wb of rat protein1:500 diluted for wb of rat protein ,the band is strong and clear!
Date published: 2017-09-27
Rated 5 out of 5 by from Great!I received this as a free sample. I used it at a 1:1000 dilution, and the secondary GAM at 1:1000 dilution on mouse and rat liver homogenates that were run on a 8% gel transferred to nitrocellulose membrane. The antibody was very clean with very little background.
Date published: 2017-09-26
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RARα/Retinoic Acid Receptor α 抗体 (C-1) is rated 4.4 out of 5 by 12.
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