Date published: 2025-9-8
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RANKL Antibody (G-1) - Western Blotting - Image 352415
RANKL 抗体 (G-1): sc-377079. HeLa (A) 和 HUV-EC-C (B) 全细胞裂解液中 RANKL 表达的 Western 印迹分析.
RANKL 抗体 (G-1): sc-377079. HeLa (A) 和 HUV-EC-C (B) 全细胞裂解液中 RANKL 表达的 Western 印迹分析.

RANKL 抗体 (G-1): sc-377079

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说明书
  • RANKL 抗体 G-1 是小鼠单克隆 IgG1 κ，RANKL抗体, 在44篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长RANKL的氨基酸46-317
  • RANKL 抗体 (G-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 RANKL
  • 抗RANKL抗体（G-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RANKL (G-1): sc-377079的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是RANKL Antibody (G-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与RANKL Antibody (G-1) 打包提供（请参阅下面的订购信息）。

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基因信息
说明书与实验方案
研究信息

関連項目

RANK抗体

RANKL抗体（G-1）是一种IgG1 κ 小鼠单克隆RANKL抗体（也称为TNFSF11抗体、TNF超家族成员11抗体或CD254抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的RANKL蛋白。RANKL抗体（G-1）既可作为非偶联抗RANKL抗体，也可作为多种偶联形式的抗RANKL抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肿瘤坏死因子（TNF）受体超家族的成员与TNF受体相关因子（TRAF）家族的信号分子相互作用，以激活NFκB和JNK通路。RANK（NFκB的受体激活剂）是在树突状细胞上鉴定出的TNFR家族成员。这种I型膜受体在多种组织中表达。RANK的C末端对于RANK与TRAF2、5和6的结合是必需的，并且对于刺激NFκB的激活也是必需的。该受体的配体RANKL（也称为TRANCE、OPGL或ODF）是一种主要在淋巴组织和T细胞系中表达的II型跨膜蛋白。RANKL似乎是T细胞和破骨细胞的重要调节因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RANKL 抗体 (G-1) 参考文献:

  1. [骨质疏松症和RANKL信号]。  |  Nakamura, M. and Udagawa, N. 2011. Clin Calcium. 21: 1149-55. PMID: 21814019
  2. RANKL是骨质疏松症和癌症相关骨病临床应用的必要机会。  |  Yasuda, H. 2013. World J Orthop. 4: 207-17. PMID: 24147256
  3. RANKL靶向治疗对免疫和癌症的影响。  |  Cheng, ML. and Fong, L. 2014. Front Oncol. 3: 329. PMID: 24432249
  4. RANKL向破骨细胞前体呈递的调节机制。  |  Honma, M., et al. 2014. Curr Osteoporos Rep. 12: 115-20. PMID: 24477414
  5. RANK/RANKL通路在乳腺癌中的作用。  |  Kiesel, L. and Kohl, A. 2016. Maturitas. 86: 10-6. PMID: 26921922
  6. SH3BP2小天使症小鼠中RANKL非依赖性破骨细胞生成。  |  Kittaka, M., et al. 2020. Bone Rep. 12: 100258. PMID: 32258251
  7. 局部RANKL递送可改善双磷酸盐治疗大鼠的骨痂愈合。  |  Soundia, A., et al. 2021. Bone. 148: 115945. PMID: 33836308
  8. RANKL抑制剂可阻止纤维发育不良小鼠的病变进展并促进骨再矿化。  |  Liu, Z., et al. 2022. Bone. 156: 116301. PMID: 34952228
  9. [RANKL的发现及其当前和未来的研究影响]。  |  Yasuda, H. 2023. Nihon Yakurigaku Zasshi. 158: 247-252. PMID: 37121707
  10. RANKL信号传导促使骨骼肌氧化。  |  Cavalcanti de Araújo, PH., et al. 2024. J Bone Miner Res. 39: 753-764. PMID: 38619281

订购信息

产品名称产品编号规格价格数量收藏夹

RANKL 抗体 (G-1)

sc-377079
200 µg/ml
RMB2377.00

RANKL (G-1): m-IgG Fc BP-HRP 套装

sc-529968
200 µg Ab; 10 µg BP
RMB2662.00

RANKL (G-1): m-IgGκ BP-HRP 套装

sc-523176
200 µg Ab, 40 µg BP
RMB2662.00

RANKL (G-1): m-IgG1 BP-HRP 套装

sc-543794
200 µg Ab; 20 µg BP
RMB2662.00

RANKL 抗体 (G-1) AC

sc-377079 AC
500 µg/ml, 25% agarose
RMB3129.00

RANKL 抗体 (G-1) HRP

sc-377079 HRP
200 µg/ml
RMB2377.00

RANKL 抗体 (G-1) FITC

sc-377079 FITC
200 µg/ml
RMB2482.00

RANKL 抗体 (G-1) PE

sc-377079 PE
200 µg/ml
RMB2580.00

RANKL 抗体 (G-1) Alexa Fluor® 488

sc-377079 AF488
200 µg/ml
RMB2685.00

RANKL 抗体 (G-1) Alexa Fluor® 546

sc-377079 AF546
200 µg/ml
RMB2685.00

RANKL 抗体 (G-1) Alexa Fluor® 594

sc-377079 AF594
200 µg/ml
RMB2685.00

RANKL 抗体 (G-1) Alexa Fluor® 647

sc-377079 AF647
200 µg/ml
RMB2685.00

RANKL 抗体 (G-1) Alexa Fluor® 680

sc-377079 AF680
200 µg/ml
RMB2685.00

RANKL 抗体 (G-1) Alexa Fluor® 790

sc-377079 AF790
200 µg/ml
RMB2685.00

Hello! I purshed conjugate antibody aganist RANKL 377079HRP in order to detect RANKL in a bone sample by IHC(P) and I followed the immunoperoxidase staining protocol that you provide on-line. However, the processing is not working. Can you help me?

Asked by: Valesca
Thank you for your question. Please reach out to your local distributor directly for assistance troubleshooting your IHC results. They will be happy to help troubleshoot and discuss your results with you, and will work with Santa Cruz as needed.
Answered by: Tech Service
Date published: 2019-08-06

Does it work well on primary cell lysates?

Asked by: flowersf
Thank you for your question. RANKL Antibody (G-1): sc-377079 is recommended for detection of RANKL of mouse, rat and human origin by WB, IP, IF and ELISA. This recommendation is valid for lysates generated from both cell cultures and tissue material.
Answered by: Tech Support Europe
Date published: 2019-07-09

Boa Tarde! Nos comentários vocês não indicam IHQ para bloco parafinado, porém nas especificações conta isso "disponível conjugado a agarose para IP; HRP para WB, IHC (P) e ELISA; e para ou ficoeritrina ou FITC para IF, IHC (P) e FCM" Estou com duvidas!

Asked by: Janaína
Thank you for your question. The conjugated form of this antibody is recommended for IHC on paraffin sections, however, the non-conjugated form has been warrantied for IF on fresh frozen tissue sections.
Answered by: Tech Service
Date published: 2019-06-20

Which is the actual molecular weight of RANKL? the data sheet says 40-35 Kd, but I have got bands around 50Kd when using primary clavaria cells from mice...

Asked by: MP03
Thank you for your question. The expected molecular weight of RANKL, as detected by RANKL Antibody (G-1): sc-377079, depends on the form present in your sample: RANKL full-length (35-40 kDa), membrane-bound RANKL (34-40 kDa), or soluble RANKL (20-30 kDa) . If you are detecting a signal at a different height, please contact our technical service for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

What's the difference between the sc-52950 and the sc-377079 HRP in IHC with mouse tissue? And what about the result?Will they be same? 

Asked by: sumer
Thank you for your inquiry. Both sc-52950 and sc-377079 are mouse monoclonal antibodies recommended for detection of RANKL of mouse, rat and human origin. sc-52950 can be used on paraffin-embedded tissues, while sc-377079 is not recommended for such application. sc-377079 HRP is sc-377079 directly conjugated to HRP. When using sc-377079 HRP in WB or IHC, the secondary reagent can be avoided. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website. Best of luck with your research!
Answered by: Technical Support 14
Date published: 2017-07-10

How much protein do you recommend loading on the gel for Western blot detection using RANKL (G-1): sc-377079 monoclonal antibody?

Asked by: jenniferc
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-02-02
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Rated 5 out of 5 by from Ottimo funzionamentoL'anticorpo funziona bene per immunoprecipitazione
Date published: 2024-10-17
Rated 3 out of 5 by from RANKL - Dot BlotRANKL Antibody works also for Dot Blot. The Antibody have been diluted 1:1000 in TBS-T buffer.
Date published: 2019-11-04
Rated 3 out of 5 by from RAW 264.7Hard to work in the WB of osteoclast induced from RAW 264.7，with 1：500.
Date published: 2017-10-23
Rated 4 out of 5 by from Good bandsThis antibody works fine on western blot in 1:500. The bands are clear.
Date published: 2017-08-25
Rated 5 out of 5 by from good performanceI used it for IF on HeLa cells (1:300 dilution). It works both on PFA and methanol-fixed cells.
Date published: 2017-05-28
Rated 5 out of 5 by from specific bandUsed this antibody to make WB in 1:600 dilution, we got obtained specific band.
Date published: 2017-05-27
Rated 5 out of 5 by from strong bands.Strong bands detected in WB for human lung tissue, use dilution 1:400.Recommend use it.
Date published: 2017-02-09
Rated 5 out of 5 by from recombinant RANKLIt works great in WB, favorable band achieved in mouse recombinant RANKL.
Date published: 2017-02-07
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RANKL 抗体 (G-1) is rated 4.3 out of 5 by 15.
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