Date published: 2025-9-8
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Ran GAP1 Antibody (C-5) - Western Blotting - Image 381354
Ran GAP1 抗体 (C-5): sc-28322. MCF7 (A), SK-BR-3 (B) 和 MDA-MB-231 (C) 全细胞裂解液中 Ran GAP1 表达的 Western 印迹分析.
Ran GAP1 抗体 (C-5): sc-28322. MCF7 (A), SK-BR-3 (B) 和 MDA-MB-231 (C) 全细胞裂解液中 Ran GAP1 表达的 Western 印迹分析.

Ran GAP1 抗体 (C-5): sc-28322

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说明书
  • Ran GAP1 抗体 C-5 是小鼠单克隆 IgG1 κ，Ran GAP1抗体, 在30篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Ran GAP1的氨基酸408-587
  • Ran GAP1 抗体 (C-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Ran GAP1
  • 抗Ran GAP1抗体（C-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ran GAP1 (C-5): sc-28322的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Ran GAP1 抗体 (C-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ran GAP1 抗体 (C-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Ran GAP1抗体（C-5）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类的Ran GAP1蛋白。Ran GAP1 (C-5) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Ran GAP1，又称Ran GTP酶激活蛋白1，在调节蛋白质和RNA穿过核膜方面发挥着至关重要的作用，这对于维持细胞功能和稳态至关重要。Ran GAP1主要位于细胞核中，参与GTP水解为GDP的过程，从而控制Ran GTPase的活跃和非活跃状态。这种调节对于DNA复制和有丝分裂以及通过核孔复合体运输大分子等过程至关重要。Ran GAP1和RCC1之间的相互作用促进了GDP与GTP的交换，凸显了Ran GAP1在细胞信号传导通路中的重要性。通过调节活性Ran GTP的水平，Ran GAP1确保细胞过程高效执行，使Ran GAP1成为细胞周期调控和核质运输的关键参与者。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ran GAP1 抗体 (C-5) 参考文献:

  1. 哺乳动物细胞中 RanGAP1 的细胞分布受 CRM1 介导的核输出调控  |  Cha, K., et al. 2015. PLoS One. 10: e0141309. PMID: 26506250
  2. RanGAP1 的缺失会导致骨肉瘤的染色体不稳定和快速肿瘤发生。  |  Gong, Y., et al. 2023. Dev Cell. 58: 192-210.e11. PMID: 36696903
  3. METTL3 介导的 RanGAP1 通过招募 YTHDF1，通过 MAPK 通路促进结直肠癌的进展。  |  Yang, R., et al. 2024. Cancer Gene Ther. 31: 562-573. PMID: 38267624
  4. RanGAP1 可在骨骼发育过程中维持肢芽间充质细胞染色体的稳定性。  |  Huang, M., et al. 2024. Cell Signal. 120: 111222. PMID: 38729327
  5. Ran 的 GTP 水解发生在核孔复合体中，是蛋白质导入的早期步骤。  |  Melchior, F., et al. 1995. J Cell Biol. 131: 571-81. PMID: 7593180
  6. Ran/TC4 GTPase 结合结构域：通过表达克隆进行鉴定并确定保守序列基序。  |  Beddow, AL., et al. 1995. Proc Natl Acad Sci U S A. 92: 3328-32. PMID: 7724562
  7. 蛋白质从细胞核输出需要 GTP 酶 Ran 和 GTP 水解。  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
  8. 核 GTP 结合蛋白 Ran 与其调控蛋白 RCC1 和 RanGAP1 的相互作用。  |  Klebe, C., et al. 1995. Biochemistry. 34: 639-47. PMID: 7819259
  9. Ran-GTP 结合蛋白 RanBP1 共同激活 RanGTP 酶并抑制 GTP 解离。  |  Bischoff, FR., et al. 1995. EMBO J. 14: 705-15. PMID: 7882974
  10. 核 Ras 相关蛋白 Ran 的 GDP 结合型晶体结构。  |  Scheffzek, K., et al. 1995. Nature. 374: 378-81. PMID: 7885480
  11. Ran GTPase的不同结构域与不同因子相互作用，调控核蛋白质导入和RNA加工。  |  Ren, M., et al. 1995. Mol Cell Biol. 15: 2117-24. PMID: 7891706
  12. RCC1 催化 Ran 的鸟嘌呤核苷酸交换，Ran-GAP1 刺激 Ran 结合的 GTP 的水解。  |  Bischoff, FR. and Ponstingl, H. 1995. Methods Enzymol. 257: 135-44. PMID: 8583915

订购信息

产品名称产品编号规格价格数量收藏夹

Ran GAP1 抗体 (C-5)

sc-28322
200 µg/ml
$316.00

Ran GAP1 (C-5): m-IgG Fc BP-HRP 套装

sc-528423
200 µg Ab; 10 µg BP
$354.00

Ran GAP1 (C-5): m-IgGκ BP-HRP 套装

sc-520797
200 µg Ab, 40 µg BP
$354.00

Ran GAP1 (C-5): m-IgG1 BP-HRP 套装

sc-542924
200 µg Ab; 20 µg BP
$354.00

Ran GAP1 抗体 (C-5) AC

sc-28322 AC
500 µg/ml, 25% agarose
$416.00

Ran GAP1 抗体 (C-5) HRP

sc-28322 HRP
200 µg/ml
$316.00

Ran GAP1 抗体 (C-5) FITC

sc-28322 FITC
200 µg/ml
$330.00

Ran GAP1 抗体 (C-5) PE

sc-28322 PE
200 µg/ml
$343.00

Ran GAP1 抗体 (C-5) Alexa Fluor® 488

sc-28322 AF488
200 µg/ml
$357.00

Ran GAP1 抗体 (C-5) Alexa Fluor® 546

sc-28322 AF546
200 µg/ml
$357.00

Ran GAP1 抗体 (C-5) Alexa Fluor® 594

sc-28322 AF594
200 µg/ml
$357.00

Ran GAP1 抗体 (C-5) Alexa Fluor® 647

sc-28322 AF647
200 µg/ml
$357.00

Ran GAP1 抗体 (C-5) Alexa Fluor® 680

sc-28322 AF680
200 µg/ml
$357.00

Ran GAP1 抗体 (C-5) Alexa Fluor® 790

sc-28322 AF790
200 µg/ml
$357.00

Can I get a free vial of this antibody ?

Asked by: CJ13
Thank you for your question. Please contact our Technical Service Department to arrange for a free 10 µg sample of Ran GAP1 (C-5): sc-28322.
Answered by: Tech Support Europe
Date published: 2021-02-09

I am using Ran GAP1 (C-5): sc-28322 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Works good for WB in HEK293TUsed this antibody for WB. Works very good for HEK293T cells. Both (90 kDa and 70 kDa) band work very good.
Date published: 2020-11-04
Rated 5 out of 5 by from Works good for IP and IF in Hela cellsUsed this antibody for WB, IP and IF. Works very good for Hela and ARPE cells
Date published: 2019-02-26
Rated 5 out of 5 by from good productThis antibody works pretty well for western blot in our lab.
Date published: 2018-05-09
Rated 4 out of 5 by from Good for WBit works well for WB in our lab, not a bad choice.
Date published: 2017-09-18
Rated 5 out of 5 by from Good separationDistinct and separate bands seen for SUMO-1/Ran GAP1 Complex and cytoplasmic Ran GAP1 in Western Blot.
Date published: 2017-01-23
Rated 4 out of 5 by from good antibodyworks good at 1:500 dilution. Clean bands of both sumoylated and unsumoylated protein.
Date published: 2016-11-23
Rated 5 out of 5 by from Excellent results with western blotExcellent results with western blot
Date published: 2015-12-02
Rated 4 out of 5 by from Working well so farWorking well so far.
Date published: 2015-10-07
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Ran GAP1 抗体 (C-5) is rated 4.7 out of 5 by 10.
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