Date published: 2025-9-7

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Ran BP-3 抗体 (C-5): sc-373678

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说明书
  • Ran BP-3抗体(C-5)是小鼠单克隆IgG1 κ, 在3篇文献中引用,规格为200 µg/ml
  • 针对human起源的Ran BP-3内部区域内的氨基酸101-244进行
  • 推荐用于 mouse, rat 和 human 来源的Ran BP-3 WB, IP, IF, IHC(P) 和 ELISA检测
  • 1 BP-HRP">m-IgG1 BP-HRP是Ran BP-3 Antibody (C-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Ran BP-3 Antibody (C-5) 打包提供(请参阅下面的订购信息)。

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小Ras相关蛋白Ran,也称为TC4,是一种核定位的GTP酶,参与多种细胞过程,包括DNA复制、有丝分裂的进入和退出以及RNA和蛋白质通过核孔复合物的运输。与Ras一样,活性Ran GTP和失活Ran GDP水平受到鸟嘌呤核苷酸交换因子(GEFs)和GTP酶激活蛋白(GAPs)的严格调控。丰富的GEF,RCC1(染色体浓缩调节因子1),增加了Ran交换GDP为GTP的速率。Ran BP-3作为一种支架蛋白,促进输出复合物的有效组装。具体而言,Ran BP-3在Ran存在的情况下促进Crm1与RCC1的结合。Ran BP-3也被证明可以结合β-catenin,从而抑制Wnt信号通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ran BP-3 抗体 (C-5) 参考文献:

  1. RanBP3 含有一个不寻常的核定位信号,它优先被输入蛋白-α3 输入。  |  Welch, K., et al. 1999. Mol Cell Biol. 19: 8400-11. PMID: 10567565
  2. Ran 结合蛋白 3 将 Crm1 与 Ran 鸟嘌呤核苷酸交换因子连接起来。  |  Nemergut, ME., et al. 2002. J Biol Chem. 277: 17385-8. PMID: 11932251
  3. AKT激活可促进卵巢癌细胞中CSE1L的核定位和促癌转录物的激活。  |  Lorenzato, A., et al. 2013. Exp Cell Res. 319: 2627-36. PMID: 23948303
  4. 鞘氨醇激酶 1 是一种促病毒因子,可在流感病毒感染时调节病毒 RNA 合成和病毒核糖核蛋白复合物的核输出。  |  Seo, YJ., et al. 2013. PLoS One. 8: e75005. PMID: 24137500
  5. Ran结合蛋白3的核定位信号结合的importin-α3晶体结构。  |  Koyama, M. and Matsuura, Y. 2017. Biochem Biophys Res Commun. 491: 609-613. PMID: 28760339
  6. Ran结合蛋白3与人类精子发生和男性不育有关。  |  Tang, W., et al. 2020. Andrologia. 52: e13446. PMID: 31833110
  7. RANBP3L缺失会导致肾上皮细胞向肾透明细胞癌样表型转化。  |  Chernyakov, D., et al. 2021. J Exp Clin Cancer Res. 40: 226. PMID: 34233711
  8. RanBP3通过调节SMAD2/3和ERK1/2的核转运来调节慢性髓细胞白血病细胞的增殖、凋亡和化疗敏感性。  |  Li, Q., et al. 2021. Front Oncol. 11: 698410. PMID: 34504783
  9. 通过基因和蛋白质表达分析比较年轻和老年雌激素受体阳性乳腺癌的生物学。  |  Siddig, A., et al. 2023. Biomedicines. 11: PMID: 36672708
  10. Ran结合蛋白3的磷酸化将Ras和PI3激酶通路与核质运输联系起来。  |  Yoon, SO., et al. 2023. Mol Cell. 83: 4190. PMID: 37980093

订购信息

产品名称产品编号规格价格数量收藏夹

Ran BP-3 抗体 (C-5)

sc-373678
200 µg/ml
RMB2377.00

Ran BP-3 (C-5): m-IgG1 BP-HRP 套装

sc-542695
200 µg Ab; 20 µg BP
RMB2662.00

Can Ran BP-3 (C-5): sc-373678 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, Ran BP-3 (C-5): sc-373678 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Strong and specific band in HEK293T Western blotsGave strong and specific band in HEK293T western blots. Works for both whole cell lysate and nuclear lysate. Recommend.
Date published: 2017-01-26
Rated 5 out of 5 by from Great immunofluorescence nuclear stainingGreat immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-09
Rated 5 out of 5 by from Robust signal in western blot analysis of Ran BPRobust signal in western blot analysis of Ran BP-3 expression in Y79 and WI 38 whole cell lysates. -SCBT QC
Date published: 2015-01-15
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Ran BP-3 抗体 (C-5) is rated 5.0 out of 5 by 3.
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