Date published: 2025-9-7
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Ran BP-3 Antibody (52) - Western Blotting - Image 63293
Ran BP-3 抗体 (52): sc-136055. WI 38 (A) 和 Y79 (B) 全细胞裂解液以及 U-937 (C), Jurkat (D) 和 Y79 (E) 核提取物中 Ran BP-3 表达的 Western 印迹分析.
Ran BP-3 抗体 (52): sc-136055. WI 38 (A) 和 Y79 (B) 全细胞裂解液以及 U-937 (C), Jurkat (D) 和 Y79 (E) 核提取物中 Ran BP-3 表达的 Western 印迹分析.

Ran BP-3 抗体 (52): sc-136055

4.7(3)
寫評論提問

说明书
  • Ran BP-3 抗体 52 是小鼠单克隆 IgG1，提供 50 µg/0.5 ml
  • 免疫human物种的Ran BP-3 isoform 2的氨基酸30-225
  • 推荐用于 human 来源的Ran BP-3 isoform 2 and Ran BP-3 isoform 3 (also designated Ran BP-3a and Ran BP-3b, respectively) WB, IP 和 IF检测
  • 目前，我们还没有完成Ran BP-3 抗体 (52)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    描述
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    関連項目

    此外 Ran BP 抗体包括 Ran BP-1, Ran BP-2, Ran BP-3 和 Ran BP-M

    小Ras相关蛋白Ran，也称为TC4，是一种核定位的GTP酶，参与多种细胞过程，包括DNA复制、有丝分裂的进入和退出以及RNA和蛋白质通过核孔复合物的运输。与Ras一样，活性Ran GTP和失活Ran GDP水平受到鸟嘌呤核苷酸交换因子（GEFs）和GTP酶激活蛋白（GAPs）的严格调控。丰富的GEF，RCC1（染色体浓缩调节因子1），增加了Ran交换GDP为GTP的速率。Ran BP-3作为支架蛋白促进输出复合物的有效组装。具体而言，Ran BP-3在Ran存在的情况下促进Crm1与RCC1的结合。Ran BP-3也被证明与β-catenin结合，从而抑制Wnt信号通路。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Ran BP-3 抗体 (52) 参考文献:

    1. Ran结合蛋白3是Crm1介导的核蛋白出口的辅助因子。  |  Lindsay, ME., et al. 2001. J Cell Biol. 153: 1391-402. PMID: 11425870
    2. Ran 结合蛋白 3 将 Crm1 与 Ran 鸟嘌呤核苷酸交换因子连接起来。  |  Nemergut, ME., et al. 2002. J Biol Chem. 277: 17385-8. PMID: 11932251
    3. 酿酒酵母GTP酶复合物：Gtr1p-Gtr2p通过酿酒酵母Ran结合蛋白Yrb2p调节细胞增殖。  |  Wang, Y., et al. 2005. Biochem Biophys Res Commun. 336: 639-45. PMID: 16143306
    4. Ran结合蛋白3磷酸化将Ras和PI3激酶通路与核质运输联系起来。  |  Yoon, SO., et al. 2008. Mol Cell. 29: 362-75. PMID: 18280241
    5. Ran结合蛋白3在甲型流感病毒复制过程中的作用。  |  Predicala, R. and Zhou, Y. 2013. J Gen Virol. 94: 977-984. PMID: 23303829
    6. RanBP3 通过选择性控制核输出调节黑色素瘤细胞增殖  |  Pathria, G., et al. 2016. J Invest Dermatol. 136: 264-74. PMID: 26763446
    7. Ran结合蛋白3的核定位信号结合的importin-α3晶体结构。  |  Koyama, M. and Matsuura, Y. 2017. Biochem Biophys Res Commun. 491: 609-613. PMID: 28760339
    8. Ran结合蛋白3与人类精子发生和男性不育有关。  |  Tang, W., et al. 2020. Andrologia. 52: e13446. PMID: 31833110
    9. NU2058通过直接靶向RanBP3阻断核β-Catenin信号诱导细胞衰老以抑制结直肠肿瘤发生  |  Zheng, CC., et al. 2022. Adv Sci (Weinh). 9: e2202528. PMID: 36270974
    10. Ran结合蛋白3的磷酸化将Ras和PI3激酶通路与核质运输联系起来。  |  Yoon, SO., et al. 2023. Mol Cell. 83: 4190. PMID: 37980093

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    Ran BP-3 抗体 (52)

    sc-136055
    50 µg/0.5 ml
    		RMB2377.00

    I am using Ran BP-3 (52): sc-136055 for immunofluorescence, what dilution of this antibody should I use?

    Asked by: AbPolly
    Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear staining in HeLa cells. -SCBT QC
    Date published: 2015-05-18
    Rated 4 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of Ran BP-3 expression in HeLa whole cell lysate. -SCBT QC
    Date published: 2015-04-17
    Rated 5 out of 5 by from Robust signal observed in western blot analysisRobust signal observed in western blot analysis of Ran BP-3 expression in WI 38 and Y79 whole cell lysates and U-937, Jurkat and Y79 nuclear extracts. -SCBT QC
    Date published: 2015-03-12
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    Ran BP-3 抗体 (52) is rated 4.7 out of 5 by 3.
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