Date published: 2025-9-7
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Ran BP-1 Antibody (D-8) - Western Blotting - Image 364120
Ran BP-1 抗体 (D-8): sc-374352. F9 (A), c4 (B), KNRK (C) 和 3611-RF (D) 全细胞裂解液中 Ran BP-1 表达的 Western 印迹分析.
Ran BP-1 抗体 (D-8): sc-374352. F9 (A), c4 (B), KNRK (C) 和 3611-RF (D) 全细胞裂解液中 Ran BP-1 表达的 Western 印迹分析.

Ran BP-1 抗体 (D-8): sc-374352

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寫評論提問

说明书
  • Ran BP-1 抗体 D-8 是小鼠单克隆 IgG1 κ，Ran BP-1抗体, 在3篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列159-203位于mouse物种的Ran BP-1的C-terminus
  • Ran BP-1 抗体 (D-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Ran BP-1
  • 抗Ran BP-1抗体（D-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ran BP-1 (D-8): sc-374352的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是Ran BP-1 Antibody (D-8) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Ran BP-1 Antibody (D-8) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Ran BP抗体，包括Ran BP-1, Ran BP-2, Ran BP-3和Ran BP-M

Ran BP-1抗体（D-8）是一种IgG1 κ小鼠单克隆Ran BP-1抗体（也称为Ran BP-1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的Ran BP-1蛋白。Ran BP-1抗体（D-8）既可以是非偶联的抗Ran BP-1抗体形式，也可以是多种偶联形式的抗Ran BP-1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。小Ras相关蛋白Ran，也称为TC4，是一种核定位的GTP酶，参与多种细胞过程，包括DNA复制、进入和退出有丝分裂以及RNA和蛋白质通过核孔复合物的转运。与Ras一样，活性Ran GTP和非活性Ran GDP水平受到鸟苷酸交换因子（GEFs）和GTP酶激活蛋白（GAPs）的严格调控。丰富的GEF、RCC1（染色体凝集调节因子1）提高了Ran交换GDP为GTP的速率。Ran GAP1通过提高Ran水解GTP为GDP的速率来抵消RCC1的作用。一种称为Ran BP1的蛋白质没有内在的GAP活性，并作为GEF抑制剂使RCC1失活，从而间接增加Ran GDP与Ran GTP的比例。Ran BP2蛋白已被提议作为核孔复合物外围的Ran GTP停靠位点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ran BP-1 抗体 (D-8) 参考文献:

  1. Ran突变体与Ran结合蛋白1结合能力不足，可刺激核出口并抑制核进口。  |  Kehlenbach, RH., et al. 2001. J Biol Chem. 276: 14524-31. PMID: 11278834
  2. 烟草（Nicotiana benthamiana）RanBP1-1通过调控小GTP酶Ran的核-细胞质转运，参与诱导抗病性。  |  Mizuno, Y., et al. 2019. Front Plant Sci. 10: 222. PMID: 30906303
  3. RanBP1：肺癌和胶质瘤中癌症干细胞的潜在治疗靶点  |  Kahm, YJ., et al. 2023. Int J Mol Sci. 24: PMID: 37047826
  4. Ran 的 GTP 水解发生在核孔复合体中，是蛋白质导入的早期步骤。  |  Melchior, F., et al. 1995. J Cell Biol. 131: 571-81. PMID: 7593180
  5. Ran/TC4 GTPase 结合结构域：通过表达克隆进行鉴定并确定保守序列基序。  |  Beddow, AL., et al. 1995. Proc Natl Acad Sci U S A. 92: 3328-32. PMID: 7724562
  6. 蛋白质从细胞核输出需要 GTP 酶 Ran 和 GTP 水解。  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
  7. 核 GTP 结合蛋白 Ran 与其调控蛋白 RCC1 和 RanGAP1 的相互作用。  |  Klebe, C., et al. 1995. Biochemistry. 34: 639-47. PMID: 7819259
  8. Ran-GTP 结合蛋白 RanBP1 共同激活 RanGTP 酶并抑制 GTP 解离。  |  Bischoff, FR., et al. 1995. EMBO J. 14: 705-15. PMID: 7882974
  9. 核 Ras 相关蛋白 Ran 的 GDP 结合型晶体结构。  |  Scheffzek, K., et al. 1995. Nature. 374: 378-81. PMID: 7885480
  10. Ran GTPase的不同结构域与不同因子相互作用，调控核蛋白质导入和RNA加工。  |  Ren, M., et al. 1995. Mol Cell Biol. 15: 2117-24. PMID: 7891706
  11. Ran结合蛋白RanBP1的胞质定位离不开核出口信号。  |  Richards, SA., et al. 1996. J Cell Biol. 134: 1157-68. PMID: 8794858
  12. Ran结合蛋白5（RanBP5）与核转运因子importin-beta有关，但与RanBP1的相互作用不同。  |  Deane, R., et al. 1997. Mol Cell Biol. 17: 5087-96. PMID: 9271386

订购信息

产品名称产品编号规格价格数量收藏夹

Ran BP-1 抗体 (D-8)

sc-374352
200 µg/ml
RMB2377.00

Ran BP-1 (D-8): m-IgG Fc BP-HRP 套装

sc-525954
200 µg Ab; 10 µg BP
RMB2662.00

Ran BP-1 抗体 (D-8) AC

sc-374352 AC
500 µg/ml, 25% agarose
RMB3129.00

Ran BP-1 抗体 (D-8) HRP

sc-374352 HRP
200 µg/ml
RMB2377.00

Ran BP-1 抗体 (D-8) FITC

sc-374352 FITC
200 µg/ml
RMB2482.00

Ran BP-1 抗体 (D-8) PE

sc-374352 PE
200 µg/ml
RMB2580.00

Ran BP-1 抗体 (D-8) Alexa Fluor® 488

sc-374352 AF488
200 µg/ml
RMB2685.00

Ran BP-1 抗体 (D-8) Alexa Fluor® 546

sc-374352 AF546
200 µg/ml
RMB2685.00

Ran BP-1 抗体 (D-8) Alexa Fluor® 594

sc-374352 AF594
200 µg/ml
RMB2685.00

Ran BP-1 抗体 (D-8) Alexa Fluor® 647

sc-374352 AF647
200 µg/ml
RMB2685.00

Ran BP-1 抗体 (D-8) Alexa Fluor® 680

sc-374352 AF680
200 µg/ml
RMB2685.00

Ran BP-1 抗体 (D-8) Alexa Fluor® 790

sc-374352 AF790
200 µg/ml
RMB2685.00

what are the dilutions that I should use for Ranbp1 in Immunofluorescence, 1:50 or 1:100? And for WB? what is the dilution to get a good signal? Shall I incubate it at 37 degree or 4 degree overnight?

Asked by: IF AB
Thank you for your question. We recommend a dilution range of 1:50-1:500 for immunofluorescence with a starting dilution of 1:50, and a dilution range of 1:100-1:1000 for western blotting with a starting dilution of 1:100. Incubation at +4 °C over night is recommended.
Answered by: Tech Support Europe
Date published: 2021-03-09

Hi, I am using this antibody for WB in 1:500 ratio and i am finding it difficult to recognize RanBP1. I am using MCF-7 cells, what is your recommendation for the ratio needed?

Asked by: DanaD
Thank you for your question. We are sorry to hear that this antibody is not performing for you as expected. We recommend a starting dilution of 1:200 for this antibody. Please reach out to our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody and help you achieve the best results possible!
Answered by: Tech Service
Date published: 2017-06-26

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-374352: Ran BP-1 (D-8) mouse monoclonal antibody will work?

Asked by: Professor Griffin
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from a useful antibody!This antibody is very useful! In WB, the band is clear and specific. In IF, the flourecence is markeble at 1:100.
Date published: 2017-09-06
Rated 5 out of 5 by from Produced nice Western blot data of Ran BPProduced nice Western blot data of Ran BP-1 expression in NIH/3T3 and F9 whole cell lysates. -SCBT QC
Date published: 2014-04-29
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Ran BP-1 抗体 (D-8) is rated 5.0 out of 5 by 2.
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