Date published: 2025-9-7
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Rad52 Antibody (F-7) - Immunofluorescence - Image 293928
Rad52 抗体 (F-7): sc-365341. 福尔马林固定的 Hep G2 细胞免疫荧光染色, 显示核定位.
Rad52 抗体 (F-7): sc-365341. 福尔马林固定的 Hep G2 细胞免疫荧光染色, 显示核定位.

Rad52 抗体 (F-7): sc-365341

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寫評論提問

说明书
  • Rad52 抗体 F-7 是小鼠单克隆 IgG1 κ，Rad52抗体, 在60篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列119-418位于human物种的Rad52的C-terminus
  • Rad52 抗体 (F-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Rad52
  • 抗Rad52抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rad52 (F-7): sc-365341的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Rad52 抗体 (F-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Rad52 抗体 (F-7) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Rad 抗体 包括 Rad1, Rad17, Rad18, Rad21, Rad23A, Rad23B, Rad26L, Rad50, Rad51和Rad51B

Rad52抗体（F-7）是一种IgG1 κ小鼠单克隆Rad52抗体（也称为DNA修复蛋白RAD52同源抗体、RAD52（酿酒酵母）同源抗体、横纹肌肉瘤抗原抗体或重组蛋白RAD52抗体），可通过蛋白质印迹法、免疫沉淀法、免疫荧光法、免疫组织化学（石蜡切片）和酶联免疫吸附测定法检测小鼠、大鼠和人类来源的Rad52蛋白。Rad52抗体（F-7）既有非偶联型抗Rad52抗体形式，也有多种偶联型抗Rad52抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Rad52家族成员（Rad50、Rad51B/C/D、Rad52、Rad54、MRE11）介导DNA双链断裂修复（DSBR），以修复可能导致细胞死亡、突变或肿瘤转化的DNA损伤。Rad51（RECA，BRCC5）与BRCA1和BRCA2相互作用，影响亚细胞定位和细胞对DNA损伤的反应。BRCA2失活可能是导致基因组不稳定和Rad51失调引发肿瘤发生的关键事件。Rad52形成一个七聚体环，与单链DNA末端结合，并催化DNA-DNA相互作用，这是互补链退火所必需的。Rad52可以与Rad51相互作用。DEAD样解旋酶超家族的Rad54A与双链DNA结合并诱导DNA拓扑结构改变，这被认为有助于同源DNA配对，并刺激DNA重组。DEAD样解旋酶超家族的Rad54B与双链DNA结合，并在DNA存在下显示ATPase活性。Rad54B在睾丸和脾脏中含量丰富，该基因的突变发生在原发性淋巴瘤和结肠癌中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rad52 抗体 (F-7) 参考文献:

  1. Brca1无效成纤维细胞的无细胞提取物中存在非同源末端连接活性缺陷。  |  Zhong, Q., et al. 2002. Cancer Res. 62: 3966-70. PMID: 12124328
  2. 单个 Rad52 修复中心多条 DNA 双链断裂的共定位。  |  Lisby, M., et al. 2003. Nat Cell Biol. 5: 572-7. PMID: 12766777
  3. Rad52, Rad54 和 Rad55 蛋白在 Rad51 介导的重组中的体内作用。  |  Sugawara, N., et al. 2003. Mol Cell. 12: 209-19. PMID: 12887906
  4. 双链断裂修复过程中 Rad51 和 Rad52 复合物的体内组装和解体。  |  Miyazaki, T., et al. 2004. EMBO J. 23: 939-49. PMID: 14765116
  5. 人类 Rap1 蛋白复合物与端粒长度的调节。  |  O'Connor, MS., et al. 2004. J Biol Chem. 279: 28585-91. PMID: 15100233
  6. 由 Rad52 和 Rad59 蛋白介导的 DNA 的退火。  |  Wu, Y., et al. 2006. J Biol Chem. 281: 15441-9. PMID: 16565518
  7. RAD50, RAD51, RAD52 及相关基因突变对酿酒酵母中非法重组的影响  |  Tsukamoto, Y., et al. 1996. Genetics. 142: 383-91. PMID: 8852838

订购信息

产品名称产品编号规格价格数量收藏夹

Rad52 抗体 (F-7)

sc-365341
200 µg/ml
RMB2377.00

Rad52 (F-7): m-IgG Fc BP-HRP 套装

sc-527357
200 µg Ab; 10 µg BP
RMB2662.00

Rad52 (F-7): m-IgG1 BP-HRP 套装

sc-532730
200 µg Ab; 20 µg BP
RMB2662.00

Rad52 抗体 (F-7) AC

sc-365341 AC
500 µg/ml, 25% agarose
RMB3129.00

Rad52 抗体 (F-7) HRP

sc-365341 HRP
200 µg/ml
RMB2377.00

Rad52 抗体 (F-7) FITC

sc-365341 FITC
200 µg/ml
RMB2482.00

Rad52 抗体 (F-7) PE

sc-365341 PE
200 µg/ml
RMB2580.00

Rad52 抗体 (F-7) Alexa Fluor® 488

sc-365341 AF488
200 µg/ml
RMB2685.00

Rad52 抗体 (F-7) Alexa Fluor® 546

sc-365341 AF546
200 µg/ml
RMB2685.00

Rad52 抗体 (F-7) Alexa Fluor® 594

sc-365341 AF594
200 µg/ml
RMB2685.00

Rad52 抗体 (F-7) Alexa Fluor® 647

sc-365341 AF647
200 µg/ml
RMB2685.00

Rad52 抗体 (F-7) Alexa Fluor® 680

sc-365341 AF680
200 µg/ml
RMB2685.00

Rad52 抗体 (F-7) Alexa Fluor® 790

sc-365341 AF790
200 µg/ml
RMB2685.00

Which conditions do you use to observe DNA damage-dependent Rad52 foci formation?

Asked by: isaw
Thank you for your question. Please contact our Technical Service Department if you have any specific questions about the data we show for this antibody on our website. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2022-11-25

What dilution do you use for IF?

Asked by: platon
Thank you for your question. We used this mouse monoclonal, Rad52 Antibody (F-7): sc-365341, at a dilution of 1:50 for immunofluorescent staining. The recommended range is 1:50-1:500, and optimal conditions should be determined by titrating the antibody in your application. A link to our recommended protocol for IF is provided below. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-16

Can you recommend a positive control to use with Rad52 (F-7): sc-365341 monoclonal antibody for Western blot?

Asked by: jenniferc
We recommend HCT 116 (sc-364175), SK-MEL-28 (sc-2236), HeLa (sc-2200), HEK 293 (sc-45136) or Raji (sc-364236) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Rad52 in these cell lines.
Answered by: Technical Support 15
Date published: 2017-01-11

What secondary is best to use with Rad52 (F-7): sc-365341 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Rad52 (F-7): sc-365341, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-11
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Rated 4 out of 5 by from Good for western blotThis antibody is good for WB. After long term storage, it can get weeker. Longer exposure is recommended.
Date published: 2019-03-03
Rated 2 out of 5 by from Not appropriate for IFThe antibody shows a pan-nuclear staining, but this is regardless of DNA damage treatment. I could not see any damage-specific foci formation.
Date published: 2018-03-16
Rated 4 out of 5 by from Worked well for WBThis Ab worked great for WB. For IF, I did get signal from my staining but did not get nice foci unlike picture shown in website.
Date published: 2018-02-01
Rated 5 out of 5 by from Great antibodyGood and clear wb detection in MCF7 cell lines using 1:200 in 70ug of protein.
Date published: 2017-11-29
Rated 5 out of 5 by from Excellent antibody!!We are getting into new areas of research and Rad52 is a protein of interest on three of our projects. The antibody provided to us by SantaCruz as a sample wroked well for us, for Rad52 positive and negative cell line.
Date published: 2017-11-17
Rated 5 out of 5 by from Great for WBWorks great for WB at 1:1000 dilution in HeLa and 293T cells.
Date published: 2017-09-06
Rated 4 out of 5 by from Clean bandUsing film and ECL. Clean band detected, at recommended dilution, after several minute exposure. Long but reliable.
Date published: 2017-05-30
Rated 4 out of 5 by from Good antibodyNice sharp band in HeLa stable cell line after whole cell lysis. We used 1:1000 as dilution and we had signal after less than 20 secs in ChemiDoc using ECL.
Date published: 2017-04-13
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Rad52 抗体 (F-7) is rated 4.4 out of 5 by 16.
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