Date published: 2025-9-8

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Rad21 抗体 (B-2): sc-271601

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说明书
  • Rad21 抗体 B-2 是小鼠单克隆 IgM κ,Rad21抗体, 在6篇文献中引用,规格为200 µg/ml
  • 针对human起源的Rad21内部区域内的氨基酸143-352进行
  • 推荐用于 mouse, rat 和 human 来源的Rad21 WB, IP, IF 和 ELISA检测; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 关于如何获取Rad21 (B-2): sc-271601免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Rad21 Antibody (B-2) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Rad21 Antibody (B-2) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

Rad21抗体(B-2)是一种IgM κ小鼠单克隆Rad21抗体(也称为Rad21抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Rad21蛋白。Rad21抗体(B-2)为非偶联的抗Rad21抗体。Rad21是主要的粘连亚基之一,将姐妹染色单体结合在一起直到后期,此时,类似半胱天冬酶的分离酶对其进行蛋白水解切割,从而实现染色体分离。RAD21与REC8相互作用形成粘连复合物,该复合物在姐妹染色单体排列中发挥作用。RAD21还参与DNA双链断裂的修复,并对有丝分裂生长至关重要。在细胞凋亡之前,Rad21会受到不同刺激诱导的C端切割。切割产物迁移到细胞质中,参与凋亡途径的早期事件,并以正反馈的方式放大细胞死亡信号。RAD21基因与癌细胞的侵袭和转移有关,并且RAD21是癌症治疗药物的潜在靶标,可能通过诱导DNA损伤来增强化疗药物的抗肿瘤活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rad21 抗体 (B-2) 参考文献:

  1. 人类肿瘤细胞的 rad21 基因 hHR21(SP) 因缺氧而下调。  |  Sook Kim, M., et al. 2001. Biochem Biophys Res Commun. 281: 1106-12. PMID: 11243849
  2. STAG2 和 Rad21 哺乳动物有丝分裂粘合蛋白与减数分裂有关。  |  Prieto, I., et al. 2002. EMBO Rep. 3: 543-50. PMID: 12034751
  3. 小鼠粘合素亚基 Rad21 和 Rec8 的 mRNA 表达模式分析:雌雄小鼠生殖细胞中 rec8 mRNA 的特异性表达。  |  Lee, J., et al. 2002. Zoolog Sci. 19: 539-44. PMID: 12130806
  4. 连接姐妹染色单体内聚与细胞凋亡:Rad21的作用  |  Pati, D., et al. 2002. Mol Cell Biol. 22: 8267-77. PMID: 12417729
  5. 凝聚素 Rad21 和 SCP3 参与了小鼠减数分裂 I 期间姐妹动核的单极附着。  |  Parra, MT., et al. 2004. J Cell Sci. 117: 1221-34. PMID: 14970259
  6. 有丝分裂期 RAD21/SCC1 凝聚蛋白在小鼠减数分裂染色体凝聚和分离中的新作用。  |  Xu, H., et al. 2004. EMBO Rep. 5: 378-84. PMID: 15031719
  7. 抑制 RAD21 基因的表达会降低人乳腺癌细胞的生长速度,并增强依托泊苷和博莱霉素的细胞毒性。  |  Atienza, JM., et al. 2005. Mol Cancer Ther. 4: 361-8. PMID: 15767545
  8. 口腔鳞状细胞癌中癌细胞的侵袭和转移与 RAD21 基因表达的相关性。  |  Yamamoto, G., et al. 2006. Virchows Arch. 448: 435-41. PMID: 16416296
  9. 拟南芥三种 RAD21 联合蛋白的特征揭示了对电离辐射的不同反应。  |  da Costa-Nunes, JA., et al. 2006. J Exp Bot. 57: 971-83. PMID: 16488915

订购信息

产品名称产品编号规格价格数量收藏夹

Rad21 抗体 (B-2)

sc-271601
200 µg/ml
RMB2377.00

Rad21 (B-2): m-IgGκ BP-HRP 套装

sc-521993
200 µg Ab, 40 µg BP
RMB2662.00

What is the recommended fixation method for immunofluorescence staining with Rad21 (B-2): sc-271601 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from goodIt works well in WB from 293T whole cell lysate using either m-IgGκ BP-HRP: sc-516102 or mouse IgM-HRP.
Date published: 2021-07-02
Rated 5 out of 5 by from WB效果还可以WB,在肠癌细胞中1:500的稀释比可以孵出来一些条带,但比较弱,不知道这种蛋白是不是需要特殊裂解。
Date published: 2018-12-17
Rated 5 out of 5 by from RAD21 抗体我一个月之前申请了RAD21抗体,货号为sc271601,很高兴,申请成功并且很快到货,最近提取了蛋白样品做了western,结果挺不错的
Date published: 2018-02-01
Rated 5 out of 5 by from Good!Good for WB in MCF7. for wetern blot this one works beautifully.
Date published: 2017-11-30
Rated 5 out of 5 by from GreatThe antibody can be used for IF staining. I think it is pretty good.
Date published: 2017-07-25
Rated 5 out of 5 by from Worked well with mouse spleen extractsWorked well with mouse spleen extracts
Date published: 2015-10-01
Rated 5 out of 5 by from Strong band of interest recognized in WBStrong band of interest recognized in WB using HeLa and NIH/3T3 nuclear extracts. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2013-06-09
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Rad21 抗体 (B-2) is rated 5.0 out of 5 by 8.
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