Date published: 2025-9-6
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RACK1 Antibody (B-3) - Western Blotting - Image 378757
RACK1 抗体 (B-3): sc-17754. C2C12 (A), NIH/3T3 (B), HeLa (C), Raji (D), U-2 OS (E) 和 Caco-2 (F) 全细胞裂解液中 RACK1 表达的 Western 印迹分析.
RACK1 抗体 (B-3): sc-17754. C2C12 (A), NIH/3T3 (B), HeLa (C), Raji (D), U-2 OS (E) 和 Caco-2 (F) 全细胞裂解液中 RACK1 表达的 Western 印迹分析.

RACK1 抗体 (B-3): sc-17754

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寫評論提問

说明书
  • RACK1 抗体 B-3 是小鼠单克隆 IgG2a κ，RACK1抗体, 在92篇文献中引用，规格为200 µg/ml
  • 免疫human物种的RACK1的氨基酸131-317
  • RACK1 抗体 (B-3) 推荐用于WB, IP, IF 和 IHC(P) ，检测 mouse, rat, human 和 来源的RACK1; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗RACK1抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RACK1 (B-3): sc-17754的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 RACK1 抗体 (B-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RACK1 抗体 (B-3) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 RACK 抗体 包括 RACK7

RACK1抗体（B-3）是一种小鼠单克隆IgG2a kappa轻链抗体，专门针对RACK1（也称为激活的C激酶1受体）和GNB2L1的氨基酸131-317。RACK1是一种317个氨基酸的多功能支架蛋白，类似于G蛋白β亚基，在细胞生长、分化、信号转导等多种细胞过程中发挥着关键作用。RACK1是激活的蛋白激酶C（PKC）的锚定蛋白，可促进PKC的转位和与底物的相互作用，这是调节信号通路以控制基因表达、激素分泌和膜功能的重要过程。通过抑制Src酪氨酸激酶和与多种信号分子相互作用，RACK1可调节细胞粘附、迁移和增殖的重要通路，因此RACK1在癌症和其他疾病的研究中具有重要意义。抗RACK1抗体（B-3）可与小鼠、大鼠、人类以及其他物种（包括马、犬、牛、猪和鸟类）的RACK1发生反应，建议用于蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）。RACK1作为支架蛋白，在协调多种信号通路方面发挥着至关重要的作用，而且RACK1在多种物种中均存在，这凸显了其在细胞基本功能中的重要性。值得注意的是，RACK1的缺陷可能与衰老大脑中PKC激活受损有关，这凸显了RACK1在神经学研究中的重要性。因此，RACK1 (B-3)抗体为推进关键细胞机制的研究和探索潜在的治疗靶点提供了宝贵的工具，有助于理解与RACK1相关的疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RACK1 抗体 (B-3) 参考文献:

  1. 蛋白激酶 C 家族的新成员 nPKC theta 主要在骨骼肌中表达。  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
  2. 信号转导蛋白激酶家族（蛋白激酶 C 家族）的结构和功能多样性；两类不同的 PKC，即传统的 cPKC 和新型的 nPKC。  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
  3. 蛋白激酶 C-delta 的表达和特征。  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
  4. 膜磷脂对多功能蛋白激酶的钙依赖性激活。  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
  5. 肌醇磷脂的周转和信号转导  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
  6. 蛋白激酶 C 在细胞表面信号转导和肿瘤促进中的作用。  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
  7. 蛋白激酶 C 可能是促进肿瘤生长的植物醇酯的受体蛋白。  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
  8. 促进肿瘤生长的植物醇酯直接激活钙激活的磷脂依赖性蛋白激酶。  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651

订购信息

产品名称产品编号规格价格数量收藏夹

RACK1 抗体 (B-3)

sc-17754
200 µg/ml
$316.00

RACK1 (B-3): m-IgG Fc BP-HRP 套装

sc-528297
200 µg Ab; 10 µg BP
$354.00

RACK1 (B-3): m-IgGκ BP-HRP 套装

sc-520638
200 µg Ab, 40 µg BP
$354.00

RACK1 (B-3): m-IgG2a BP-HRP 套装

sc-547062
200 µg Ab; 10 µg BP
$354.00

RACK1 抗体 (B-3) AC

sc-17754 AC
500 µg/ml, 25% agarose
$416.00

RACK1 抗体 (B-3) HRP

sc-17754 HRP
200 µg/ml
$316.00

RACK1 抗体 (B-3) FITC

sc-17754 FITC
200 µg/ml
$330.00

RACK1 抗体 (B-3) PE

sc-17754 PE
200 µg/ml
$343.00

RACK1 抗体 (B-3) Alexa Fluor® 488

sc-17754 AF488
200 µg/ml
$357.00

RACK1 抗体 (B-3) Alexa Fluor® 546

sc-17754 AF546
200 µg/ml
$357.00

RACK1 抗体 (B-3) Alexa Fluor® 594

sc-17754 AF594
200 µg/ml
$357.00

RACK1 抗体 (B-3) Alexa Fluor® 647

sc-17754 AF647
200 µg/ml
$357.00

RACK1 抗体 (B-3) Alexa Fluor® 680

sc-17754 AF680
200 µg/ml
$357.00

RACK1 抗体 (B-3) Alexa Fluor® 790

sc-17754 AF790
200 µg/ml
$357.00

Do polymers occur during WB? Are there any reported cases?

Asked by: Anonymous
感谢您的提问。 我们目前没有发现这个蛋白的抗体在进行WB实验时，有二聚体或者三聚体出现。 Thank you for your question. We looked at some publications from PubMed and we only see one single band for RACK1. We do not see a any for a dimer or trimer .
Answered by: Ling Chen
Date published: 2024-03-28

Does this Rack1 monoclonal Ab work against C. elegans rack-1 like your discontinued rabbit polyclonal Ab?

Asked by: DDCsta
Thank you for your question. This monoclonal antibody was raised against the same antigen used to develop the discontinued rabbit polyclonal antibody, sc-10775: RACK1 (H-187). We do not have any data or citations yet using this monoclonal antibody in C. elegans.
Answered by: Technical Support
Date published: 2019-05-25

I have tried this antibody FITC conjugated against mouse RACK1 in flow cytometry application. It did not appear to have any signal. Do you know if this antibody has been tested against mouse Rack1 in flow? Thanks!

Asked by: JZ2019
This antibody has not yet been tested for Flow cytometry (FCM), so this is not a recommended application at this time. The antibody is recommended for detection of RACK1 by WB, IP and IHC. The antibody is mouse, rat and human reactive.
Answered by: Tech Service
Date published: 2019-03-28

Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, our RACK1 sc-17754 is available in a variety of direct conjugations, such as HRP, PE, FITC, AlexaFluor 488 and AlexaFluor 647.
Answered by: Technical Support 8
Date published: 2017-01-04
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Rated 4 out of 5 by from Nice in western blotThe antibody works nicely in Western blotting on 25 μg of whole-cell lysates from the human cell line HEK-293.
Date published: 2023-11-09
Rated 5 out of 5 by from Nice and single band for WBThis ab works very well in COS7, Hela, and MDA-MB-231 cells. Detects a single band around 35kDa. Ab incubation: 1:1000 in 5% milk in TBST for 2h at RT.
Date published: 2021-04-30
Rated 5 out of 5 by from greatworks very well for U2OS cells. Detects a strong single band around 35kDa
Date published: 2019-10-22
Rated 5 out of 5 by from Great!This works very well in WB (5% milk, 1:200). Very specific and strong signals.
Date published: 2018-11-13
Rated 5 out of 5 by from Strong and specific signalThis antibody works really well. Using the dilution 1:1000 and 1:5000-1:7000 for the secondary Ab I have got a strong signal at 36 kDa and no background.
Date published: 2018-03-26
Rated 5 out of 5 by from works very wellworks great for western - samples were human cell in culture
Date published: 2017-11-21
Rated 5 out of 5 by from Good for IFThis RACK1 antibody shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2017-11-06
Rated 5 out of 5 by from Anti-RACK1 antibodyMouse anti-RACK1 antibody (sc--17754) works great on immunoblotting at 1:1000 in 2.5 % milk powder (and can be reused up to 4 times). It works well on IHC at 1:200 and works great on IP. It recognizes a 35 kDa band in all cell lines tested so far.
Date published: 2017-08-24
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RACK1 抗体 (B-3) is rated 5.0 out of 5 by 23.
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