Date published: 2025-9-7
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Rac GAP1 Antibody (A-6) - Western Blotting - Image 359591
Rac GAP1 抗体 (A-6): sc-271110. Jurkat (A), K-562 (B), F9 (C) 和 H19-7/IGF-IR (D) 全细胞裂解液中 Rac GAP1 表达的 Western 印迹分析.
Rac GAP1 抗体 (A-6): sc-271110. Jurkat (A), K-562 (B), F9 (C) 和 H19-7/IGF-IR (D) 全细胞裂解液中 Rac GAP1 表达的 Western 印迹分析.

Rac GAP1 抗体 (A-6): sc-271110

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说明书
  • Rac GAP1 抗体 A-6 是小鼠单克隆 IgG2a κ，Rac GAP1抗体, 在17篇文献中引用，规格为200 µg/ml
  • 针对氨基酸142-437的图谱，在Rac GAP1的内部区域，human起源
  • Rac GAP1 抗体 (A-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Rac GAP1
  • 抗Rac GAP1抗体（A-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rac GAP1 (A-6): sc-271110的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Rac GAP1 抗体 (A-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Rac GAP1 抗体 (A-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Rac GAP1抗体（A-6）是一种IgG2aκ型小鼠单克隆Rac GAP1抗体（也称为Rac GAP1抗体），可以通过WB、IP、IF、IHC（P）和ELISA等方法检测到小鼠、大鼠和人类来源的Rac GAP1蛋白。Rac GAP1抗体（A-6）可以以非共轭型抗Rac GAP1抗体形式获得，也可以以多种共轭型抗Rac GAP1抗体形式获得，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。Ras超家族中已鉴定出大量低分子量的GTP结合蛋白。这些蛋白质调节所有真核细胞中许多基本过程，如生长、囊泡转运和细胞骨架组织。GTPase激活蛋白（GAPs）加速Ras相关蛋白的GTP水解的内在速率，从而导致其活性形式的下调。通过这种功能，GAPs负性调节Ras介导的信号传导。Rac GAP1（Rac GTPase激活蛋白1），也称为MgcRacGAP（男性生殖细胞Rac GTPase激活蛋白）、ID-GAP或HsCYK-4，作为GAP发挥作用，并对Rac 1和Cdc42表现出强烈的活性。Rac GAP1在胸腺、胎盘和睾丸中高表达，在脾脏和外周血淋巴细胞中表达水平较低。它包含一个Rho-GAP域和一个佛波醇酯/DAG型锌指。Rac GAP1在细胞分裂中起着至关重要的作用，既作为支架蛋白又作为GTPase调节剂。在细胞分裂期间，Rac GAP1在多个位点被磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rac GAP1 抗体 (A-6) 参考文献:

  1. Tat1，一种特异性表达于人类雄性生殖细胞并可能与 rhogtpase 信号转导有关的新型硫酸盐转运体。  |  Toure, A., et al. 2001. J Biol Chem. 276: 20309-15. PMID: 11278976
  2. 转基因小鼠前列腺转移性神经内分泌细胞癌的分子特征。  |  Hu, Y., et al. 2002. J Biol Chem. 277: 44462-74. PMID: 12228243
  3. 利用基因表达阵列和递归后裔分区分析筛选上皮性卵巢癌的潜在标记物。  |  Lu, KH., et al. 2004. Clin Cancer Res. 10: 3291-300. PMID: 15161682
  4. 人类 ARHGAP10 基因的特征研究。  |  Katoh, M. and Katoh, M. 2004. Int J Oncol. 25: 1201-6. PMID: 15375573
  5. ARHGAP27基因的鉴定和特征描述。  |  Katoh, Y. and Katoh, M. 2004. Int J Mol Med. 14: 943-7. PMID: 15492870
  6. MgcRacGAP 控制着收缩环的组装和细胞分裂的启动。  |  Zhao, WM. and Fang, G. 2005. Proc Natl Acad Sci U S A. 102: 13158-63. PMID: 16129829
  7. 有丝分裂过程中 MgcRacGAP 的磷酸化调节涉及极光 B 和 Cdk1 蛋白激酶以及 PP2A 磷酸酶。  |  Touré, A., et al. 2008. FEBS Lett. 582: 1182-8. PMID: 18201571
  8. Rac GTPase-activating protein 1的上调与人类肝细胞癌的早期复发密切相关。  |  Wang, SM., et al. 2011. Clin Cancer Res. 17: 6040-51. PMID: 21825042
  9. RACGAP1 上调通过 PI3K/AKT 途径促进 GABPA 调节的肝细胞癌进展。  |  Gu, Y., et al. 2022. Oxid Med Cell Longev. 2022: 3034150. PMID: 35958019
  10. circ_rac GTPase-Activating Protein 1通过多嘧啶核苷酸结合蛋白1的招募促进非小细胞肺癌的干细胞性和转移，从而促进Sirtuin-3介导的复制时间调节因子1的脱乙酰化。  |  Xiong, H., et al. 2023. Lab Invest. 103: 100010. PMID: 36748197
  11. 靶向RACGAP1可抑制生长激素垂体腺瘤的生长。  |  Sun, F., et al. 2024. Endocrine.. PMID: 39607642
  12. MgcRacGAP 是一种新的人类 Rac 和 Cdc42 GTPase 激活蛋白，与果蝇 rotundRacGAP 基因产物相似，在雄性生殖细胞中表达。  |  Touré, A., et al. 1998. J Biol Chem. 273: 6019-23. PMID: 9497316

订购信息

产品名称产品编号规格价格数量收藏夹

Rac GAP1 抗体 (A-6)

sc-271110
200 µg/ml
RMB2377.00

Rac GAP1 (A-6): m-IgG Fc BP-HRP 套装

sc-529122
200 µg Ab; 10 µg BP
RMB2662.00

Rac GAP1 (A-6): m-IgGκ BP-HRP 套装

sc-521850
200 µg Ab, 40 µg BP
RMB2662.00

Rac GAP1 (A-6): m-IgG2a BP-HRP 套装

sc-547266
200 µg Ab; 10 µg BP
RMB2662.00

Rac GAP1 抗体 (A-6) AC

sc-271110 AC
500 µg/ml, 25% agarose
RMB3129.00

Rac GAP1 抗体 (A-6) HRP

sc-271110 HRP
200 µg/ml
RMB2377.00

Rac GAP1 抗体 (A-6) FITC

sc-271110 FITC
200 µg/ml
RMB2482.00

Rac GAP1 抗体 (A-6) PE

sc-271110 PE
200 µg/ml
RMB2580.00

Rac GAP1 抗体 (A-6) Alexa Fluor® 488

sc-271110 AF488
200 µg/ml
RMB2685.00

Rac GAP1 抗体 (A-6) Alexa Fluor® 546

sc-271110 AF546
200 µg/ml
RMB2685.00

Rac GAP1 抗体 (A-6) Alexa Fluor® 594

sc-271110 AF594
200 µg/ml
RMB2685.00

Rac GAP1 抗体 (A-6) Alexa Fluor® 647

sc-271110 AF647
200 µg/ml
RMB2685.00

Rac GAP1 抗体 (A-6) Alexa Fluor® 680

sc-271110 AF680
200 µg/ml
RMB2685.00

Rac GAP1 抗体 (A-6) Alexa Fluor® 790

sc-271110 AF790
200 µg/ml
RMB2685.00

I am using Rac GAP1 (A-6): sc-271110 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from ottimo funzionamentoHo comprato questo anticorpo per testarlo in Western blot su lisato di cellule endoteliali umane e funziona molto bene
Date published: 2021-10-11
Rated 4 out of 5 by from Works in chickenThis antibody works by in whole mount immunohistochemistry on chicken embryos at a 1:100 dilution.
Date published: 2018-03-26
Rated 5 out of 5 by from a fantastic antibody for both WB and IFThis antibody recognizes a clear and strong band of predicted M.W. on WB (1:1000 dilution, 40 ug HeLa extract). It also works well for IF using either formaldehyde or methanol-fixed HeLa cells (1:1000 dilution).
Date published: 2017-09-03
Rated 5 out of 5 by from Antibody is the best I've ever used.Antibody is the best I've ever used,so I decide to buy another one.
Date published: 2017-04-28
Rated 4 out of 5 by from For Western BlotI used the RacGAP1 Antibody（A-6)，sc-271110 for WB of human cervical cancer cells(Hela,Caski,Siha)， using the dilution1:100， the result was pretty good. The Molecular Weight was about 70kDa. And the background was clear.
Date published: 2017-04-23
Rated 5 out of 5 by from recommend useStrong band in western blot analysis of human samples of Rac GAP1, perfect experiment, we recommend the use of monoclonal antibodies.
Date published: 2017-02-12
Rated 5 out of 5 by from Recommend to everyone.Good specificity. got a pretty good impression of our lab.
Date published: 2017-02-10
Rated 5 out of 5 by from The Best AntibodyRac GAP1 antibody has strong single band in WB in mouse peritoneal macrophages, good specificity.We are in a very good place in that regard.
Date published: 2017-02-07
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Rac GAP1 抗体 (A-6) is rated 4.8 out of 5 by 11.
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