Date published: 2025-9-7

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Rab 32 抗体 (B-4): sc-390178

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说明书
  • Rab 32 抗体 B-4 是小鼠单克隆 IgG1 κ,Rab 32抗体, 在6篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列181-223位于mouse物种的Rab 32的C-terminus
  • Rab 32 抗体 (B-4) 推荐用于WB, IP, IF 和 ELISA,检测mouse 来源的 Rab 32
  • 抗Rab 32抗体(B-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rab 32 (B-4): sc-390178免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Rab 32 Antibody (B-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Rab 32 Antibody (B-4) 打包提供(请参阅下面的订购信息)。

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関連項目

Rab 32抗体(B-4)是一种IgG1 κ小鼠单克隆Rab 32抗体(也称为RAB32抗体),可通过WB、IP、IF和ELISA检测小鼠来源的Rab 32蛋白。Rab 32抗体(B-4)既有非偶联的抗Rab 32抗体形式,也有多种偶联形式的抗Rab 32抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。RAB家族的小GTP结合蛋白在囊泡和颗粒靶向中起着至关重要的作用。Rab32是一个含有225个氨基酸的蛋白质,属于小GTP酶超家族和Rab家族。Rab32具有不寻常的GTP结合序列DIAGQE,而不是更常见的DTAGQE。Rab32通过与蛋白激酶A的II型调节亚基结合并将其锚定到线粒体上,从而起到A激酶锚定蛋白的作用。Rab32还参与线粒体分裂的同步,在心脏、肝脏、肾脏、骨髓、睾丸、结肠和胎儿肺中高表达。已发现Rab32在微卫星不稳定性高(MSI-H)的结肠癌中经常发生超甲基化。尽管Rab32甲基化在子宫内膜癌中很少见,但它与胃腺癌中的hMLH1超甲基化和MSI密切相关。Rab32基因在黑猩猩、牛、小鼠、大鼠、鸡、斑马鱼、果蝇、蚊子和秀丽隐杆线虫中是保守的,并定位于人类染色体6q24.3。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rab 32 抗体 (B-4) 参考文献:

  1. Rab32通过其效应蛋白网状蛋白3L(reticulon 3L)触发线粒体相关膜(MAM)蛋白的自噬降解。  |  Herrera-Cruz, MS., et al. 2021. Biol Direct. 16: 22. PMID: 34743744
  2. B16-F1黑色素瘤细胞中酪氨酸酶向黑素体的依赖性和非依赖性转运。  |  Nishizawa, A., et al. 2022. Int J Mol Sci. 23: PMID: 36430618
  3. 破骨细胞和巨噬细胞中Rab32和Rab38阳性溶酶体相关细胞器的表征。  |  Noda, K., et al. 2023. J Biol Chem. 299: 105191. PMID: 37625588
  4. Rab32和Rab38通过调节破骨细胞中的细胞内运输来维持骨骼稳态。  |  Tokuda, K., et al. 2023. Cell Struct Funct. 48: 223-239. PMID: 37793839
  5. Rab32家族蛋白调节自噬体成分的回收。  |  Wu, Z., et al. 2024. J Cell Biol. 223: PMID: 38323995
  6. 解读慢性阻塞性肺病中RAB32/GPRC5A轴的分子调控。  |  Wu, Y., et al. 2024. Respir Res. 25: 116. PMID: 38448858
  7. LYSMD蛋白促进Rab32家族GTP酶的激活,从而促进溶酶体相关细胞器的生物发生。  |  Li, J., et al. 2024. J Cell Biol. 223: PMID: 39078368
  8. 帕金森病中的RAB32突变。  |  Monfrini, E., et al. 2024. Lancet Neurol. 23: 961-962. PMID: 39304252
  9. 帕金森病中的RAB32突变。  |  Zhao, Y., et al. 2024. Lancet Neurol. 23: 962-963. PMID: 39304253
  10. RAB32相关帕金森病的遗传学和流行病学见解。  |  Radefeldt, M., et al. 2025. Mov Disord. 40: 147-151. PMID: 39460989

订购信息

产品名称产品编号规格价格数量收藏夹

Rab 32 抗体 (B-4)

sc-390178
200 µg/ml
$316.00

Rab 32 (B-4): m-IgG Fc BP-HRP 套装

sc-530085
200 µg Ab; 10 µg BP
$354.00

Rab 32 (B-4): m-IgGκ BP-HRP 套装

sc-523436
200 µg Ab, 40 µg BP
$354.00

Rab 32 (B-4): m-IgG1 BP-HRP 套装

sc-543861
200 µg Ab; 20 µg BP
$354.00

Rab 32 抗体 (B-4) AC

sc-390178 AC
500 µg/ml, 25% agarose
$416.00

Rab 32 抗体 (B-4) HRP

sc-390178 HRP
200 µg/ml
$316.00

Rab 32 抗体 (B-4) FITC

sc-390178 FITC
200 µg/ml
$330.00

Rab 32 抗体 (B-4) PE

sc-390178 PE
200 µg/ml
$343.00

Rab 32 抗体 (B-4) Alexa Fluor® 488

sc-390178 AF488
200 µg/ml
$357.00

Rab 32 抗体 (B-4) Alexa Fluor® 546

sc-390178 AF546
200 µg/ml
$357.00

Rab 32 抗体 (B-4) Alexa Fluor® 594

sc-390178 AF594
200 µg/ml
$357.00

Rab 32 抗体 (B-4) Alexa Fluor® 647

sc-390178 AF647
200 µg/ml
$357.00

Rab 32 抗体 (B-4) Alexa Fluor® 680

sc-390178 AF680
200 µg/ml
$357.00

Rab 32 抗体 (B-4) Alexa Fluor® 790

sc-390178 AF790
200 µg/ml
$357.00

How is the antibody coupled to the agarose beads in sc-390178 AC?   Is it covalently coupled?  Is there to elute the sample protein off the beads while leaving the antibody on the bead (i.e. acid wash or heat or SDS)?

Asked by: Emre
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-02-08

will this antibody react with rat samples?

Asked by: kim7
Thank you for your question. Based on our tests with Rab 32 Antibody (B-4): sc-390178 we can only recommend it for detection of Rab 32 of mouse origin by WB, IP, IF and ELISA.
Answered by: Technical Support Europe
Date published: 2020-10-21

What is the recommended fixation method for immunofluorescence staining with Rab 32 (B-4): sc-390178 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from works fineI tried this antibody with WB for cell lysate, it worked showing specific bands, but also show some nonspecific bands.I am not sure whether it is due to the long time storage in the shipping way.
Date published: 2018-04-04
Rated 5 out of 5 by from clear bandThis antibody gives a very clear band by WB (1/600) on HeLa cells.
Date published: 2017-05-13
Rated 5 out of 5 by from Great WB data with human transfected lysatesGreat WB data with human transfected lysates. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Great IF data with methanolGreat IF data with methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2014-08-20
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Rab 32 抗体 (B-4) is rated 4.5 out of 5 by 4.
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