Date published: 2025-9-7
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Rab 14 Antibody (D-5) - Western Blotting - Image 398119
Rab 14 抗体 (D-5): sc-271401. Rab 14 在 Hep G2 (A), HeLa (B), Raji (C), NIH/3T3 (E) 和 LADMAC (F) 全细胞裂解液和人肾组织提取物 (D) 中表达的 Western 印迹分析. 所用检测试剂: m-IgG1 BP-HRP: sc-525408.
Rab 14 抗体 (D-5): sc-271401. Rab 14 在 Hep G2 (A), HeLa (B), Raji (C), NIH/3T3 (E) 和 LADMAC (F) 全细胞裂解液和人肾组织提取物 (D) 中表达的 Western 印迹分析. 所用检测试剂: m-IgG1 BP-HRP: sc-525408.

Rab 14 抗体 (D-5): sc-271401

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寫評論提問

说明书
  • Rab 14 抗体 D-5 是小鼠单克隆 IgG1, 在11篇文献中引用，提供 200 µg/ml
  • 免疫原氨基酸序列161-215位于human物种的Rab 14的C-terminus
  • Rab 14 抗体 (D-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 来源的Rab 14; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗Rab 14抗体（D-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rab 14 (D-5): sc-271401的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Rab 14 Antibody (D-5) for WB and IHC(P) applications. 的首选辅助检测试剂，这些试剂现与Rab 14 Antibody (D-5) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Rab抗体，包括Rab 25, Rab 1, Rab 10, Rab 11, Rab 11A, Rab 12, Rab 13, Rab 14, Rab 16, Rab 18, Rab 1A, Rab 1B, Rab 2, Rab 20, Rab 21和Rab 22A

Rab 14抗体（D-5）是一种IgG1小鼠单克隆Rab 14抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人的Rab 14。Rab 14抗体（D-5）既有非偶联抗Rab 14抗体形式，也有多种偶联形式的抗Rab 14抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ras相关的鸟嘌呤核苷酸结合蛋白超家族包括R-Ras、Rap、Ral/Rec和Rho/Rab亚家族，这些亚家族都被认为在内吞作用或生物合成蛋白转运中发挥重要作用。将新合成的蛋白质从内质网（ER）转运到高尔基体的各个堆叠和分泌囊泡的过程涉及载体囊泡的运动，并需要Rab蛋白的功能。Rab蛋白也是内吞途径的组成部分。Rab 14，也称为FBP，是一种由215个氨基酸组成的蛋白质，通过脂质锚定在细胞膜的细胞质侧。作为小GTPases的Rab亚家族的几个成员之一，Rab 14被认为参与全身囊泡转运和神经递质释放，并在脑、肺、肾、脾和胸腺中高表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rab 14 抗体 (D-5) 参考文献:

  1. 通过克隆和亚细胞定位研究小GTP结合rab蛋白，揭示骨吸收破骨细胞的胞内膜贩运途径。  |  Zhao, H., et al. 2002. Biochem Biophys Res Commun. 293: 1060-5. PMID: 12051767
  2. Rab14 参与高尔基复合体和内体之间的膜贩运。  |  Junutula, JR., et al. 2004. Mol Biol Cell. 15: 2218-29. PMID: 15004230
  3. 大规模筛选新型狂犬病效应因子发现了意想不到的广泛 Rab 结合特异性。  |  Fukuda, M., et al. 2008. Mol Cell Proteomics. 7: 1031-42. PMID: 18256213
  4. 肺泡 II 型细胞中 Annexin A2 与 Rab14 的相互作用  |  Gou, D., et al. 2008. J Biol Chem. 283: 13156-64. PMID: 18332131
  5. Rab14 调节极化上皮细胞的顶端靶向。  |  Kitt, KN., et al. 2008. Traffic. 9: 1218-31. PMID: 18429929
  6. 细胞分裂过程中的膜交通和脂质域极化  |  Echard, A. 2008. Biochem Soc Trans. 36: 395-9. PMID: 18481967
  7. Rab14/MACF2复合物调节细胞分裂过程中的内体靶向。  |  Gibieža, P., et al. 2021. Mol Biol Cell. 32: 554-566. PMID: 33566684
  8. 小 GTP 酶 Rab14 调控神经酰胺从内质网到高尔基体的运输并促进经典猪瘟病毒的组装。  |  Liu, YY., et al. 2023. J Virol. 97: e0036423. PMID: 37255314
  9. RAB14 通过自噬依赖的 AKT 信号通路促进膀胱癌的上皮-间质转化。  |  Deng, H., et al. 2023. Cell Death Discov. 9: 292. PMID: 37558664
  10. RhoBTB3-SHIP164-Vps26B复合体在高尔基-内质体接触处的Rab14阳性内质体芽的生物生成。  |  Wang, J., et al. 2024. Cell Discov. 10: 38. PMID: 38565878
  11. rab亚家族三种GTP结合蛋白的分子克隆和亚细胞定位。  |  Olkkonen, VM., et al. 1993. J Cell Sci. 106 (Pt 4): 1249-61. PMID: 8126105
  12. 人类黑色素瘤细胞中表达的 RAB GTPases。  |  Chen, D., et al. 1997. Biochim Biophys Acta. 1355: 1-6. PMID: 9030196

订购信息

产品名称产品编号规格价格数量收藏夹

Rab 14 抗体 (D-5)

sc-271401
200 µg/ml
RMB2377.00

Rab 14 (D-5): m-IgG Fc BP-HRP 套装

sc-527241
200 µg Ab; 10 µg BP
RMB2662.00

Rab 14 (D-5): m-IgG1 BP-HRP 套装

sc-532614
200 µg Ab; 20 µg BP
RMB2662.00

Rab 14 抗体 (D-5) AC

sc-271401 AC
500 µg/ml, 25% agarose
RMB3129.00

Rab 14 抗体 (D-5) HRP

sc-271401 HRP
200 µg/ml
RMB2377.00

Rab 14 抗体 (D-5) FITC

sc-271401 FITC
200 µg/ml
RMB2482.00

Rab 14 抗体 (D-5) PE

sc-271401 PE
200 µg/ml
RMB2580.00

Rab 14 抗体 (D-5) Alexa Fluor® 488

sc-271401 AF488
200 µg/ml
RMB2685.00

Rab 14 抗体 (D-5) Alexa Fluor® 546

sc-271401 AF546
200 µg/ml
RMB2685.00

Rab 14 抗体 (D-5) Alexa Fluor® 594

sc-271401 AF594
200 µg/ml
RMB2685.00

Rab 14 抗体 (D-5) Alexa Fluor® 647

sc-271401 AF647
200 µg/ml
RMB2685.00

Rab 14 抗体 (D-5) Alexa Fluor® 680

sc-271401 AF680
200 µg/ml
RMB2685.00

Rab 14 抗体 (D-5) Alexa Fluor® 790

sc-271401 AF790
200 µg/ml
RMB2685.00

Are there any issues with using sc-271401: Rab 14 (D-5) mouse monoclonal primary antibody on mouse samples?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-271401 conjugates.
Answered by: Technical Support
Date published: 2017-03-07

What would the appropriate isotype control antibody be for this antibody? Mouse IgG1?

Asked by: MichaelC
Thank you for your question. The proper isotype control for our Rab 14 antibody sc-271401 would be a normal mouse IgG1, such as sc-3877 (https://www.scbt.com/scbt/product/normal-mouse-igg1).
Answered by: Technical Service
Date published: 2016-11-30
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Rated 4 out of 5 by from RAB14 AntibodyI used RAB14 coniugated with Goat anti-Mouse IgG, Alexa Fluor® 568 and gave a very specific peak using a 1:50 dilution.
Date published: 2023-01-26
Rated 5 out of 5 by from Powerful signalWe received this antibody as a free trial sample a month ago. Due to the fact that it is a little amount we only assayed one dilution on western blotting (1/500). Signal was great and the band, well defined. We are really happy with the quality of this antibody and we aim to test it on other techniques( IF and FC).
Date published: 2017-10-20
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of Rab 14 expression in LADMAC and Hep G2 whole cell lysates.
Date published: 2017-01-26
Rated 5 out of 5 by from Western Blot of LADMAC and Hep G2 whole cellWestern Blot of LADMAC and Hep G2 whole cell lysates showed very strong bands -SCBT QC
Date published: 2014-09-17
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Rab 14 抗体 (D-5) is rated 4.8 out of 5 by 4.
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