Date published: 2025-9-7
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Rab 11A Antibody (A-6) - Western Blotting - Image 357487
Rab 11A 抗体 (A-6): sc-166912. PC-12 (A), PC-3 (B) 和 Hep G2 (C) 全细胞裂解液及小鼠睾丸组织提取物 (D) 中 Rab 11A 表达的 Western 印迹分析.
Rab 11A 抗体 (A-6): sc-166912. PC-12 (A), PC-3 (B) 和 Hep G2 (C) 全细胞裂解液及小鼠睾丸组织提取物 (D) 中 Rab 11A 表达的 Western 印迹分析.

Rab 11A 抗体 (A-6): sc-166912

4.2(13)
寫評論提問

说明书
  • Rab 11A 抗体 A-6 是小鼠单克隆 IgG2b κ，Rab 11A抗体, 在24篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Rab 11A的氨基酸130-216
  • Rab 11A 抗体 (A-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Rab 11A; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗Rab 11A抗体（A-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rab 11A (A-6): sc-166912的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Rab 11A 抗体 (A-6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Rab 抗体 包括 Rab 25, Rab 1, Rab 10, Rab 11, Rab 11A, Rab 12, Rab 13, Rab 14, Rab 16和Rab 18

Rab 11A 抗体（A-6）是一种 IgG2b κ 小鼠单克隆 Rab 11A 抗体（也称为 RAB11A 抗体），可通过 WB、IP、IF、IHC(P) 和 ELISA 检测小鼠、大鼠和人源的 Rab 11A 蛋白。Rab 11A 抗体（A-6）既可以是非偶联的抗 Rab 11A 抗体形式，也可以是多种偶联形式的抗 Rab 11A 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。Ras 相关的鸟苷酸结合蛋白超家族，包括 Ral/Rec、Rap、R-Ras 和 Rho/Rab 亚家族，与 Ras p21 具有 30-60% 的同源性。积累的数据表明，Rab 蛋白在内吞作用或生物合成蛋白转运中起着重要作用。新合成的蛋白从内质网转运到高尔基复合体的各个部位以及分泌囊泡，每个阶段都涉及载体囊泡的移动，这一过程似乎与 Rab 蛋白的功能有关。Rab 蛋白也可能直接指导从分泌囊泡到质膜的胞吐作用，这一可能性得到了以下观察的支持：在酵母中，与 Rab 蛋白具有 40% 同源性的 SEC4 蛋白与分泌囊泡相关。已经鉴定出 Rab 亚家族的几个成员，每个成员都存在于膜转运途径的特定阶段。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rab 11A 抗体 (A-6) 参考文献:

  1. 通过 PCR 克隆方法揭示 Rab 和 Rho GTP 结合蛋白亚家族的复杂性。  |  Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
  2. 克隆主要在脂肪细胞中表达的 Rab3 同工型。  |  Baldini, G., et al. 1992. Proc Natl Acad Sci U S A. 89: 5049-52. PMID: 1594612
  3. 人类 Rab 基因编码的 GTP 结合蛋白家族与参与分泌的酵母 YPT1 和 SEC4 产物有关。  |  Zahraoui, A., et al. 1989. J Biol Chem. 264: 12394-401. PMID: 2501306
  4. Rab7 和 Rab9 通过生化不同的过程被招募到晚期内体上。  |  Soldati, T., et al. 1995. J Biol Chem. 270: 25541-8. PMID: 7592724
  5. 通过表位标签对 Rab8 和 Rab10 这两种低分子量 GTP 结合蛋白进行表达和定域。  |  Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
  6. 朋友和家人：Rab GTPases 在囊泡交通中的作用。  |  Novick, P. and Brennwald, P. 1993. Cell. 75: 597-601. PMID: 8242735
  7. 鉴定人类血小板中的小 GTP 结合型 rab 蛋白：凝血酶诱导的 rab3B, rab6 和 rab8 蛋白磷酸化。  |  Karniguian, A., et al. 1993. Proc Natl Acad Sci U S A. 90: 7647-51. PMID: 8356066
  8. Rab 7：晚期内细胞膜交通的重要调节器。  |  Feng, Y., et al. 1995. J Cell Biol. 131: 1435-52. PMID: 8522602
  9. Rab11 是一种位于上皮组织顶端的小 GTP 结合蛋白。  |  Goldenring, JR., et al. 1996. Am J Physiol. 270: G515-25. PMID: 8638719

订购信息

产品名称产品编号规格价格数量收藏夹

Rab 11A 抗体 (A-6)

sc-166912
200 µg/ml
RMB2377.00

Rab 11A 抗体 (A-6) AC

sc-166912 AC
500 µg/ml, 25% agarose
RMB3129.00

Rab 11A 抗体 (A-6) HRP

sc-166912 HRP
200 µg/ml
RMB2377.00

Rab 11A 抗体 (A-6) FITC

sc-166912 FITC
200 µg/ml
RMB2482.00

Rab 11A 抗体 (A-6) PE

sc-166912 PE
200 µg/ml
RMB2580.00

Rab 11A 抗体 (A-6) Alexa Fluor® 488

sc-166912 AF488
200 µg/ml
RMB2685.00

Rab 11A 抗体 (A-6) Alexa Fluor® 546

sc-166912 AF546
200 µg/ml
RMB2685.00

Rab 11A 抗体 (A-6) Alexa Fluor® 594

sc-166912 AF594
200 µg/ml
RMB2685.00

Rab 11A 抗体 (A-6) Alexa Fluor® 647

sc-166912 AF647
200 µg/ml
RMB2685.00

Rab 11A 抗体 (A-6) Alexa Fluor® 680

sc-166912 AF680
200 µg/ml
RMB2685.00

Rab 11A 抗体 (A-6) Alexa Fluor® 790

sc-166912 AF790
200 µg/ml
RMB2685.00

Hi every one,  I blocked membrane with 5% milk then inucubated with 1:500 Rab11a (sc-166912), the band is faint and there is moe background? Could any body tell me what I should use 5% milk or 2%BSA, It seems to me the problem in blocking buffer

Asked by: fawza
Thank you for your question. We recommend our UltraCruz® Blocking Reagent: sc-516214 which has been optimized for use with all of our antibody products. Generally, BSA is better for revealing faint signals, whereas milk-based blocking buffers can reduce background from promiscuous binding. You may also need to increase the amount of primary and/or secondary antibody in your experiment for a cleaner and more robust signal. Please contact our technical service team directly if issues with your experiment persist.
Answered by: Tech Support Europe
Date published: 2023-07-12

Does Rab 11A Antibody (A-6): sc-166912 only recognize Rab11A? Or can the antibody also bind to Rab11B?

Asked by: ila123
Thank you for your question. This monoclonal antibody is raised against an antigen that is specific for RAB 11A and does not cross react with Rab 11B. However, we have not tested the antibody for cross reactivity with Rab 11B specifically.
Answered by: Technical Support
Date published: 2019-02-23

I have been trying to do immuno-fluorescence with the antibody using a standard Immunostaing protocol. I have tried dilution of 1:10 also, but not got any staining. The antibody gives a good band in western blot . Please kindly suggest.

Asked by: RUCHIR
Thank you for your question. We are happy to help you in troubleshooting this antibody. Please contact our Technical Service Department for support. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2018-01-31

What is the recommended fixation method for IF with Rab 11A (A-6): sc-166912 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 8
Date published: 2017-02-03
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Rated 4 out of 5 by from It works well in IF on HepG2 cellsI used the Ab in the following conditions: Cells: HepG2_20000 cell/well MW6 Blocking: BSA 5%_2h_RT Primary Ab: Rab 11A (sc-166912)_1: 50 in BSA 1%_ ON+4 Secondary Ab: Anti mouse_FITC 1:1000 Hoechst 1: 1000 ( 2 ug/ml) per 10' Comments: DAPI OK, Rab 11 A green signal seems good
Date published: 2021-09-29
Rated 3 out of 5 by from Staining worksI got a sample and tested it. The staining which I used 1:100 dilution is working on MEF cells.
Date published: 2019-05-23
Rated 4 out of 5 by from IF観察数カ月前に提供して頂きました。動物培養細胞の顆粒マ－カ－を検索していますが、陽性マ－カ－として利用できそうなのでさらに別の陽性コントロ－ルを加え、追跡中です。
Date published: 2018-06-15
Rated 3 out of 5 by from Not goodWe received a sample and used it in human colon carcinoma cells. We could not detect a band of right size in western blot.
Date published: 2018-04-03
Rated 5 out of 5 by from Worked for WBReceived as free sample. Good detection, low background. 1/1000. Blocked in 2% BSA
Date published: 2018-03-07
Rated 5 out of 5 by from great antibody for both WB and IFWe received a sample and used it in human colon carcinoma cells. We saw cytoplasmic dots in IF and a clear band of right size in western blot.
Date published: 2017-10-25
Rated 5 out of 5 by from works great on mouse cell line cell extract. Highlworks great on mouse cell line cell extract. Highly recommended.
Date published: 2017-08-01
Rated 1 out of 5 by from Apparently it does not workBlotting on primary rat cortices (cell culture), 20 ug protein per lane, 1:50 dilution, control tubulin band excellent, Rab11a band nonexistent.
Date published: 2017-06-03
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Rab 11A抗体 (A-6) is rated 4.2 out of 5 by 13.
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