Date published: 2025-9-7

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Rab 1 抗体 (E-8): sc-515308

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说明书
  • Rab 1 抗体 E-8 是小鼠单克隆 IgG1 κ,Rab 1抗体, 在3篇文献中引用,规格为200 µg/ml
  • 与氨基酸1-205结合,代表全长Rab 1AA,源自human
  • Rab 1 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Rab 1A, Rab 1B and Rab 1C; 可能与 other Rab family members产生交联反应
  • 抗Rab 1抗体(E-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rab 1 (E-8): sc-515308免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Rab 1 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Rab 1 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Rab 1 抗体(E-8)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的 Rab 1 蛋白。抗Rab 1抗体(E-8)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Rab 1蛋白在细胞内运输中发挥着关键作用,特别是在内质网和高尔基体之间的囊泡运动中,这对蛋白质的正常分泌至关重要。这种运输过程通过确保蛋白质的正确修饰并将其输送到预定目的地来维持细胞功能和稳态。Rab蛋白调节胞吐作用,即囊泡与细胞膜融合,将内容物释放到细胞外。Rab 1在细胞膜运输途径各阶段中的特殊性凸显了其在细胞物流中的重要性,使其成为内吞作用和生物合成运输中的关键角色。通过与参与这些过程的特定蛋白相互作用,Rab 1对于理解细胞通讯和蛋白质运输的机制至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rab 1 抗体 (E-8) 参考文献:

  1. 细胞内运输中的 GTP 结合蛋白。  |  Pfeffer, SR. 1992. Trends Cell Biol. 2: 41-6. PMID: 14731525
  2. 通过 PCR 克隆方法揭示 Rab 和 Rho GTP 结合蛋白亚家族的复杂性。  |  Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
  3. 克隆主要在脂肪细胞中表达的 Rab3 同工型。  |  Baldini, G., et al. 1992. Proc Natl Acad Sci U S A. 89: 5049-52. PMID: 1594612
  4. 人类 Rab 基因编码的 GTP 结合蛋白家族与参与分泌的酵母 YPT1 和 SEC4 产物有关。  |  Zahraoui, A., et al. 1989. J Biol Chem. 264: 12394-401. PMID: 2501306
  5. 细胞信号、癌症和其他疾病中的 Rab1。  |  Yang, XZ., et al. 2016. Oncogene. 35: 5699-5704. PMID: 27041585
  6. 对 elegans 中 Rab GTPase 基因的 RNAi 筛选发现,生殖系统的体细胞依赖 rab-1 进行形态发生,但不依赖干细胞龛维持。  |  Scott, K., et al. 2024. bioRxiv.. PMID: 39677816
  7. 通过表位标签对 Rab8 和 Rab10 这两种低分子量 GTP 结合蛋白进行表达和定域。  |  Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
  8. Coatomers 和 SNAREs 在促进膜交通方面的作用。  |  Takizawa, PA. and Malhotra, V. 1993. Cell. 75: 593-6. PMID: 8242734
  9. 朋友和家人:Rab GTPases 在囊泡交通中的作用。  |  Novick, P. and Brennwald, P. 1993. Cell. 75: 597-601. PMID: 8242735
  10. GTP 结合蛋白在外排途径运输中的作用。  |  Ferro-Novick, S. and Novick, P. 1993. Annu Rev Cell Biol. 9: 575-99. PMID: 8280472
  11. 血小板聚集过程中低分子量 GTP 结合蛋白 rap2B 与细胞骨架的关联  |  Torti, M., et al. 1993. Proc Natl Acad Sci U S A. 90: 7553-7. PMID: 8356055
  12. 鉴定人类血小板中的小 GTP 结合型 rab 蛋白:凝血酶诱导的 rab3B, rab6 和 rab8 蛋白磷酸化。  |  Karniguian, A., et al. 1993. Proc Natl Acad Sci U S A. 90: 7647-51. PMID: 8356066

订购信息

产品名称产品编号规格价格数量收藏夹

Rab 1 抗体 (E-8)

sc-515308
200 µg/ml
RMB2377.00

Rab 1 (E-8): m-IgG Fc BP-HRP 套装

sc-531333
200 µg Ab; 10 µg BP
RMB2662.00

Rab 1 (E-8): m-IgGκ BP-HRP 套装

sc-524997
200 µg Ab, 40 µg BP
RMB2662.00

Rab 1 (E-8): m-IgG1 BP-HRP 套装

sc-544489
200 µg Ab; 20 µg BP
RMB2662.00

Rab 1 抗体 (E-8) AC

sc-515308 AC
500 µg/ml, 25% agarose
RMB3129.00

Rab 1 抗体 (E-8) HRP

sc-515308 HRP
200 µg/ml
RMB2377.00

Rab 1 抗体 (E-8) FITC

sc-515308 FITC
200 µg/ml
RMB2482.00

Rab 1 抗体 (E-8) PE

sc-515308 PE
200 µg/ml
RMB2580.00

Rab 1 抗体 (E-8) Alexa Fluor® 488

sc-515308 AF488
200 µg/ml
RMB2685.00

Rab 1 抗体 (E-8) Alexa Fluor® 546

sc-515308 AF546
200 µg/ml
RMB2685.00

Rab 1 抗体 (E-8) Alexa Fluor® 594

sc-515308 AF594
200 µg/ml
RMB2685.00

Rab 1 抗体 (E-8) Alexa Fluor® 647

sc-515308 AF647
200 µg/ml
RMB2685.00

Rab 1 抗体 (E-8) Alexa Fluor® 680

sc-515308 AF680
200 µg/ml
RMB2685.00

Rab 1 抗体 (E-8) Alexa Fluor® 790

sc-515308 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-515308: Rab 1 (E-8) mouse monoclonal antibody will work?

Asked by: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 1 out of 5 by from 完全显不出来从试剂商那里领取的小样,很差,显不出来,连基本条带都没有。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。
Date published: 2021-08-02
Rated 5 out of 5 by from Very Good antibodyI tested this antibody on mouse brain lysate, detected a strong protein band (around 20 kDa) in WB (1:200 dilution).
Date published: 2018-07-09
Rated 3 out of 5 by from It works for WB, but with certain background.I tried the sample, it works, but did not give very sharp bands for 293T cells, and has background.
Date published: 2018-01-29
Rated 3 out of 5 by from The signal is good but could not solve the problemThe HAP-1 cells have both Rab1A and Rab1B proteins. We have two cell lines with deleted either Rab1A or Rab1B and we need to check the deletions. There is good antibody for Rab1A and Santa Cruz use to have antibody for Rab1B (rabbit). Unfortunately, this antibody did not work for me. You can see signal, but you cannot make any conclusion about Rab1B deletion whereas G-10 antibody gives clear result for Rab1A.
Date published: 2018-01-10
Rated 3 out of 5 by from Apparently not ideal for J774A.1 cellsStained J774A.1 cells with sc-515308 followed by a green secondary antibody; got dim diffuse fluorescence, but could not confirm that it was staining the right subcellular compartment. Fortunately, this was one of a set of free samples from Santa Cruz Biotechnology, so no harm done.
Date published: 2017-04-12
Rated 1 out of 5 by from Didn't work for IF in HUVECsDid not work for Immunoflorescence (4% PFA fixed) of HUVEC cells.
Date published: 2017-03-09
Rated 3 out of 5 by from Not as effective as polyclonal antibody in WBI tested the titration of a sample monoclonal Rab1 antibody and compared with that of polyclonal Rab1b antibody, it gave a much weaker signal in WB.
Date published: 2016-12-26
Rated 4 out of 5 by from Good for ImmunofluorescenceGood Golgi staining in methanol fixed HeLa cells. Not a the strongest signal. Used antibody at a 1:50 dilution
Date published: 2016-11-29
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Rab 1 抗体 (E-8) is rated 2.9 out of 5 by 8.
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