Date published: 2025-9-7

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PTTG 抗体 (DCS-280): sc-56207

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说明书
  • PTTG 抗体 DCS-280 是小鼠单克隆 IgG2a κ,PTTG抗体, 在27篇文献中引用,规格为200 µg/ml
  • 针对全长PTTG,human起源
  • PTTG 抗体 (DCS-280) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 PTTG
  • 抗PTTG抗体(DCS-280)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PTTG (DCS-280): sc-56207免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 PTTG 抗体 (DCS-280) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与PTTG 抗体 (DCS-280) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

PTTG 抗体(DCS-280)是一种小鼠单克隆 IgG2a kappa 轻链抗体,可通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附试验(ELISA)检测小鼠、大鼠和人源的 PTTG 蛋白。PTTG (DCS-280) 抗体有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。PTTG 蛋白又称垂体瘤转化基因,在细胞周期调控中起着至关重要的作用,它是无丝分裂抑制剂,专门调节有丝分裂过程中染色体分离所必需的分离素 Esp1。这一功能有助于确保染色体的正常分离,从而防止染色体不稳定,而染色体不稳定是许多癌症的特征。PTTG 蛋白主要定位于细胞质中,但也存在于细胞核中,表明它参与多种细胞过程。PTTG 蛋白在某些癌细胞系(如 Jurkat 细胞)中过表达,并在胸腺、睾丸和胎盘等多种人体组织中被检测到。PTTG 蛋白具有独特的结构特征,包括碱性氨基末端结构域和酸性羧基末端结构域,当与其他 DNA 结合结构域融合时可发挥转录因子的功能,这进一步突出了其在细胞调控中的重要性以及在肿瘤发生过程中的潜在影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PTTG 抗体 (DCS-280) 参考文献:

  1. 无丝分裂期开始时姊妹染色单体的分离是由内聚素亚基 Scc1 的裂解促进的。  |  Uhlmann, F., et al. 1999. Nature. 400: 37-42. PMID: 10403247
  2. 鉴定参与转化和肿瘤发生的脊椎动物姐妹染色单体分离抑制因子  |  Zou, H., et al. 1999. Science. 285: 418-22. PMID: 10411507
  3. PTTG 的过表达与人类垂体腺瘤的血管生成有关。  |  Minematsu, T., et al. 2006. Endocr Pathol. 17: 143-53. PMID: 17159247
  4. [多发性骨髓瘤患者垂体肿瘤转化基因的表达]  |  Wang, Z., et al. 2006. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 14: 1143-5. PMID: 17204181
  5. PTTG 在不同实验性泌乳素瘤和人类泌乳素瘤中的表达与多巴胺能对泌乳素的控制有关。  |  Cristina, C., et al. 2007. Mol Cancer. 6: 4. PMID: 17222350
  6. PTTG 和 PBF 可抑制人类碘化钠交感器。  |  Boelaert, K., et al. 2007. Oncogene. 26: 4344-56. PMID: 17297475
  7. PTTG 过度表达可促进人类食管鳞状细胞癌的淋巴结转移。  |  Yan, S., et al. 2009. Cancer Res. 69: 3283-90. PMID: 19351864
  8. PTTG是结直肠癌细胞生长和迁移的STAT3目的基因。  |  Zhou, C., et al. 2014. Oncogene. 33: 851-61. PMID: 23416975
  9. PTTG在垂体腺瘤的发展中具有促进-抑制的双重作用。  |  Xiong, Z., et al. 2019. Protein Pept Lett. 26: 800-818. PMID: 37020362
  10. Pds1p 是芽殖酵母的无丝分裂抑制剂,在 APC 和检查点途径中发挥着关键作用。  |  Yamamoto, A., et al. 1996. J Cell Biol. 133: 99-110. PMID: 8601617
  11. hpttg 是大鼠 pttg 的人类同源物,在造血肿瘤中过度表达。有证据表明 hPTTG 具有转录激活功能。  |  Domínguez, A., et al. 1998. Oncogene. 17: 2187-93. PMID: 9811450
  12. 酵母凝聚素复合体需要一种保守蛋白 Eco1p(Ctf7) 在 DNA 复制过程中建立姐妹染色单体之间的内聚力。  |  Tóth, A., et al. 1999. Genes Dev. 13: 320-33. PMID: 9990856

订购信息

产品名称产品编号规格价格数量收藏夹

PTTG 抗体 (DCS-280)

sc-56207
200 µg/ml
$316.00

PTTG (DCS-280): m-IgGκ BP-HRP 套装

sc-521034
200 µg Ab, 40 µg BP
$354.00

PTTG 抗体 (DCS-280) AC

sc-56207 AC
500 µg/ml, 25% agarose
$416.00

PTTG 抗体 (DCS-280) HRP

sc-56207 HRP
200 µg/ml
$316.00

PTTG 抗体 (DCS-280) FITC

sc-56207 FITC
200 µg/ml
$330.00

PTTG 抗体 (DCS-280) PE

sc-56207 PE
200 µg/ml
$343.00

PTTG 抗体 (DCS-280) Alexa Fluor® 488

sc-56207 AF488
200 µg/ml
$357.00

PTTG 抗体 (DCS-280) Alexa Fluor® 546

sc-56207 AF546
200 µg/ml
$357.00

PTTG 抗体 (DCS-280) Alexa Fluor® 594

sc-56207 AF594
200 µg/ml
$357.00

PTTG 抗体 (DCS-280) Alexa Fluor® 647

sc-56207 AF647
200 µg/ml
$357.00

PTTG 抗体 (DCS-280) Alexa Fluor® 680

sc-56207 AF680
200 µg/ml
$357.00

PTTG 抗体 (DCS-280) Alexa Fluor® 790

sc-56207 AF790
200 µg/ml
$357.00

Can PTTG (DCS-280): sc-56207 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PTTG (DCS-280): sc-56207 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very good antibody for western blotGreat on human prostate cancer cells. One band with no background. Dilute at 1:1000.
Date published: 2019-05-09
Rated 5 out of 5 by from very good antibody for western blotvery clear signal,very good antibody for detecting PTTG1 in melanoma cells.
Date published: 2018-02-02
Rated 5 out of 5 by from goodWe tested on Hela cell lysate, got clean band with weak non-specific background.
Date published: 2017-10-31
Rated 5 out of 5 by from Excellent in cryosectioned human epidermisWorks really well in cryosectioned and paraformaldehyde fixed human epidermis.
Date published: 2017-08-31
Rated 5 out of 5 by from WB assayMouse testis tissue high expression of target protein PTTG,it is recommended not to loading too many tissue lysates so as not to cause high background. But for weak expression sample,the antibody should diluted more than 1:300.
Date published: 2017-07-15
Rated 5 out of 5 by from Works in WBWorks in WB, we had clear band and extra strong signal.
Date published: 2017-05-14
Rated 5 out of 5 by from Great featuresIt works very well for both Western blot (at 1:1000), the specific band is clear.
Date published: 2017-04-27
Rated 5 out of 5 by from ExcellentThis antibody is great in western blot analysis of PTTG expression in GH3 and AtT-20/D16vF2 whole cell lysates.
Date published: 2017-01-27
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PTTG 抗体 (DCS-280) is rated 5.0 out of 5 by 11.
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