Date published: 2025-9-8
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PTBP-2 Antibody (A-10) - Western Blotting - Image 377676
PTBP-2 抗体 (A-10): sc-376316. 在 HeLa (A), SJRH30 (B), MOLT-4 (C), NIH/3T3 (D) 和 A549 (E) 全细胞裂解液及大鼠睾丸组织提取物 (F) 中 PTBP-2 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
PTBP-2 抗体 (A-10): sc-376316. 在 HeLa (A), SJRH30 (B), MOLT-4 (C), NIH/3T3 (D) 和 A549 (E) 全细胞裂解液及大鼠睾丸组织提取物 (F) 中 PTBP-2 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

PTBP-2 抗体 (A-10): sc-376316

5.0(3)
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说明书
  • PTBP-2 抗体 A-10 是小鼠单克隆 IgG1 (kappa轻链)，PTBP-2抗体，规格为200 µg/ml
  • 免疫原氨基酸序列138-177位于human物种的PTBP-2的内部区域内
  • PTBP-2 抗体 (A-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PTBP-2; 也和以下物种反应，包括： 和 equine, canine and bovine
  • 抗PTBP-2抗体（A-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PTBP-2 (A-10): sc-376316的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 PTBP-2 抗体 (A-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PTBP-2 抗体 (A-10) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

PTBP-2抗体（A-10）是一种IgG1 κ小鼠单克隆PTBP-2抗体（也称为PTBP-2抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的PTBP-2蛋白。PTBP-2抗体（A-10）既可以是非偶联的抗PTBP-2抗体形式，也可以是多种偶联形式的抗PTBP-2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。PTBP-2（多嘧啶区结合蛋白2），也称为PTB或nPTB（神经多嘧啶区结合蛋白），是多嘧啶区结合蛋白家族的一员。PTBP-2主要在大脑中表达，但也存在于心脏和骨骼肌中，它定位于细胞核，并包含四个RRM（RNA识别基序）结构域。PTBP-2作为一种RNA结合蛋白，与参与调节外显子剪接和细胞质中mRNA稳定的复合物相关。由于选择性剪接事件的存在，PTBP-2有六种异构体。异构体1和2（也称为nPTB1和nPTB2/PTBPLP-L）是神经元特异性的。异构体3和4（也称为nPTB3/PTBPLP-L和nPTB4）存在于非神经元组织中，异构体5和6（也称为nPTB5/nPTB7/PTBPLP-S和nPTB6/nPTB8/PTBPLP-S）也是如此。各种异构体的存在可能起到调节PTBP-2的RNA结合特性的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PTBP-2 抗体 (A-10) 参考文献:

  1. 多嘧啶束结合蛋白的组织特异性同源物诱导 hnRNP 复合物的合作组装。  |  Markovtsov, V., et al. 2000. Mol Cell Biol. 20: 7463-79. PMID: 11003644
  2. PTBP2基因中一个组织特异性替代外显子侧翼的2kb内含子序列的进化保护。  |  Rahman, L., et al. 2004. Genomics. 83: 76-84. PMID: 14667811
  3. 多嘧啶束结合蛋白 2 通过与其 3'UTR 结合稳定小鼠雄性生殖细胞中的磷酸甘油激酶 2 mRNA。  |  Xu, M. and Hecht, NB. 2007. Biol Reprod. 76: 1025-33. PMID: 17329592
  4. PTB/nPTB转换：神经元分化编程的转录后机制。  |  Coutinho-Mansfield, GC., et al. 2007. Genes Dev. 21: 1573-7. PMID: 17606635
  5. 多嘧啶束结合蛋白的转录后调控开关重新规划了发育中神经元的替代剪接。  |  Boutz, PL., et al. 2007. Genes Dev. 21: 1636-52. PMID: 17606642
  6. 微RNA miR-124通过触发大脑特异性前mRNA剪接促进神经元分化。  |  Makeyev, EV., et al. 2007. Mol Cell. 27: 435-48. PMID: 17679093
  7. MSY2 和多嘧啶束结合蛋白 2 稳定哺乳动物睾丸中的 mRNA。  |  Xu, M. and Hecht, NB. 2008. Int J Androl. 31: 457-61. PMID: 18380784

订购信息

产品名称产品编号规格价格数量收藏夹

PTBP-2 抗体 (A-10)

sc-376316
200 µg/ml
RMB2377.00

PTBP-2 (A-10): m-IgG Fc BP-HRP 套装

sc-529851
200 µg Ab; 10 µg BP
RMB2662.00

PTBP-2 (A-10): m-IgGκ BP-HRP 套装

sc-522914
200 µg Ab, 40 µg BP
RMB2662.00

PTBP-2 抗体 (A-10) AC

sc-376316 AC
500 µg/ml, 25% agarose
RMB3129.00

PTBP-2 抗体 (A-10) HRP

sc-376316 HRP
200 µg/ml
RMB2377.00

PTBP-2 抗体 (A-10) FITC

sc-376316 FITC
200 µg/ml
RMB2482.00

PTBP-2 抗体 (A-10) PE

sc-376316 PE
200 µg/ml
RMB2580.00

PTBP-2 抗体 (A-10) Alexa Fluor® 488

sc-376316 AF488
200 µg/ml
RMB2685.00

PTBP-2 抗体 (A-10) Alexa Fluor® 546

sc-376316 AF546
200 µg/ml
RMB2685.00

PTBP-2 抗体 (A-10) Alexa Fluor® 594

sc-376316 AF594
200 µg/ml
RMB2685.00

PTBP-2 抗体 (A-10) Alexa Fluor® 647

sc-376316 AF647
200 µg/ml
RMB2685.00

PTBP-2 抗体 (A-10) Alexa Fluor® 680

sc-376316 AF680
200 µg/ml
RMB2685.00

PTBP-2 抗体 (A-10) Alexa Fluor® 790

sc-376316 AF790
200 µg/ml
RMB2685.00

The antibody sc-376316 is suitable for immunoprecipitation experiments? Is it specific to PTBP2 isoform and not isoform PTBP1 ?

Asked by: CGallego
Thank you for your question. We have not yet tested this antibody in an immunoprecipitation assay, however, we do recommend and therefore extend our product warranty to cover this research application based on our experience in developing other antibodies. This antibody is specific for PTBP-2.
Answered by: Technical Support
Date published: 2018-11-20

I am using PTBP-2 (A-10): sc-376316 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25

could you please show an IF done by this antibody, as you say it works? thank you iona

Asked by: iona
Thank you for your question. We do not currently have any published IF data for our mouse monoclonal antibody, PTBP-2 Antibody (A-10): sc-376316. We do have published Western blot and IHC(P) data on our website. We do warranty the antibody to work for IF based on our validation of the antibody in these other research applications. If you purchase the antibody and it does not work as expected in one of our recommended applications and species, then we would be happy to offer you a replacement or refund. Please contact our Technical Service department for assistance.
Answered by: Technical Support
Date published: 2016-10-20
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Rated 5 out of 5 by from Great antibody!I tested this antibody for my western blot. Using 1:2k dilution, it worked well with very high signal and no extra bands. If someone are still troubled by weak signals from similar antibodies of Abcam, I suggest trying this excellent product.
Date published: 2017-10-04
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclearProduced exceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Good Western Blot data of PTBPGood Western Blot data of PTBP-2 expression in non-transfected and human PTBP-2 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-09-18
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PTBP-2 抗体 (A-10) is rated 5.0 out of 5 by 3.
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