Date published: 2025-9-7
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PSGL-1 Antibody (HECA-452) - Immunohistochemistry - Image 284753
PSGL-1 (HECA-452: sc-53514. 对福尔马林固定, 石蜡包埋的人精囊组织进行免疫过氧化物酶染色, 显示腺细胞的胞浆染色.
PSGL-1 (HECA-452: sc-53514. 对福尔马林固定, 石蜡包埋的人精囊组织进行免疫过氧化物酶染色, 显示腺细胞的胞浆染色.

PSGL-1 抗体 (HECA-452): sc-53514

4.5(4)
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说明书
  • PSGL-1 抗体 HECA-452 是大鼠单克隆 IgM, 在1篇文献中引用，提供 200 µg/ml
  • 针对human来源的皮肤归巢记忆细胞
  • 推荐用于 mouse, rat 和 human 来源的PSGL-1 WB, IP, IF, IHC(P) 和 FCM检测; 还建议用于检测skin-homing memory T cells, binding to the T cell carbohydrate ligand for vascular E-selectin; 识别唾液酸Lewis x和唾液酸Lewis a以及E-选择素的其他碳水化合物配体，以及高内皮静脉的E-选择素结合表位
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 目前，我们还没有完成PSGL-1 抗体 (HECA-452)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 CTAGE 抗体 包括 CTAGE2, CTAGE4, CTAGE5, CTAGE6和CTAGE9

PSGL-1（P-选择素糖蛋白配体，也被称为CD162）以二硫键连接的同二聚体形式存在。PSGL-1是一种1型膜蛋白，定位于白细胞微绒毛的尖端。其细胞外结构域富含丝氨酸、苏氨酸和脯氨酸，并在HL-60和U-937细胞以及人类白细胞中分别包含一系列15和16个十聚体重复。尽管PSGL-1似乎是人类造血细胞上P-选择素的唯一受体，但它也通过独特的结合位点与E-选择素相互作用。为了将PSGL-1结合到E-选择素或P-选择素上，PSGL-1必须被唾液酸化和岩藻糖化。PSLG-1是一种类似粘蛋白的分子，非常像白细胞唾液酸糖蛋白（CD43）、CD164和CD34。这些蛋白质属于一个新兴的细胞粘附受体家族，称为唾液酸粘蛋白，它们在造血细胞中传递负信号。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

PSGL-1 抗体 (HECA-452)

sc-53514
200 µg/ml
RMB2377.00

PSGL-1 抗体 (HECA-452) FITC

sc-53514 FITC
200 µg/ml
RMB2482.00

PSGL-1 抗体 (HECA-452) PE

sc-53514 PE
200 µg/ml
RMB2580.00

I have a question regarding sc-53514. This is a rat monoclonal IgM. Would you be able to suggest a HRP conjugated secondary antibody for this product? I was wondering if I need anti rat anti IgM as secondary antibody or any anti Rat IgG will work for this

Asked by: AAAA2222
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-10

Can PSGL-1 (HECA-452): sc-53514 monoclonal antibody be used in WB with pig tissue or cells?

Asked by: nigel
Thank you for your question. This antibody has not yet been tested in pig samples and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-11-13

Can PSGL-1 (HECA-452): sc-53514 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PSGL-1 (HECA-452): sc-53514 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from sc-53514I have a question regarding sc-53514. This is a rat monoclonal IgM. Would you be able to suggest a HRP conjugated secondary antibody for this product? I was wondering if I need anti rat anti IgM as secondary antibody or any anti Rat IgG will work for this purpose?
Date published: 2022-05-09
Rated 5 out of 5 by from nice IP stainingWe used this antibody for Immunoperoxidase staining and were able to get nice images of the stained areas
Date published: 2017-02-06
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human seminal vesicle tissue. -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by from Superb indirect FCM data of human peripheralSuperb indirect FCM data of human peripheral blood leukocytes stained with PSGL-1 . -SCBT QC
Date published: 2014-09-06
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PSGL-1 抗体 (HECA-452) is rated 4.5 out of 5 by 4.
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