Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
PSCA Antibody (7F5) - Immunohistochemistry - Image 365280
PSCA 抗体 (7F5): sc-80654. 福尔马林固定, 石蜡包埋的人膀胱组织的免疫过氧化物酶染色, 显示尿道细胞膜和胞质染色.
PSCA Antibody (7F5) - Immunohistochemistry - Image 365280
PSCA 抗体 (7F5): sc-80654. 福尔马林固定, 石蜡包埋的人膀胱组织的免疫过氧化物酶染色, 显示尿道细胞膜和胞质染色.
PSCA Antibody (7F5) - Immunohistochemistry - Image 365279
PSCA 抗体 (7F5): sc-80654. 福尔马林固定, 石蜡包埋的人上胃组织的免疫过氧化物酶染色显示腺细胞的胞浆染色.
PSCA 抗体 (7F5): sc-80654. 福尔马林固定, 石蜡包埋的人膀胱组织的免疫过氧化物酶染色, 显示尿道细胞膜和胞质染色.

PSCA 抗体 (7F5): sc-80654

5.0(4)
寫評論提問

说明书
  • PSCA 抗体 7F5 是小鼠单克隆 IgG1 κ，PSCA抗体, 在13篇文献中引用，规格为200 µg/ml
  • 针对表达PSCA的human来源的P815细胞
  • PSCA 抗体 (7F5) 推荐用于WB, IF, IHC(P) 和 FCM，检测human 来源的 PSCA
  • 抗PSCA抗体（7F5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PSCA (7F5): sc-80654的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 PSCA 抗体 (7F5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PSCA 抗体 (7F5) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

PSCA抗体（7F5）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测人类来源的PSCA蛋白。PSCA (7F5) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。前列腺干细胞抗原（PSCA）是一种123个氨基酸的糖基化蛋白，属于糖基磷脂酰肌醇（GPI）锚定细胞表面抗原的Thy-1/Ly-6家族，在细胞信号传导和粘附中起着至关重要的作用。PSCA基因位于8q24.3染色体上，主要在前列腺和胎盘组织中表达。值得注意的是，PSCA在许多前列腺癌中过度表达，并且在尿路上皮肿瘤中也有大量PSCA转录本，这表明它可能成为尿路上皮和胃组织癌变的生物标志物。PSCA与特定蛋白（如c-myc）的相互作用凸显了PSCA在癌症生物学中的重要性。c-myc也位于8q染色体上，以在细胞增殖和癌症进展中的作用而闻名。在前列腺癌细胞表面抗原中，PSCA在80%以上的前列腺癌中表达，并与某些表型相关，包括前列腺癌骨转移，这使得PSCA成为治疗和诊断应用的重要靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PSCA 抗体 (7F5) 参考文献:

  1. 局部晚期前列腺癌中前列腺干细胞抗原（PSCA）和 MYC 的共扩增。  |  Reiter, RE., et al. 2000. Genes Chromosomes Cancer. 27: 95-103. PMID: 10564591
  2. 前列腺干细胞抗原（PSCA）的表达随前列腺癌的高格里森评分, 晚期和骨转移而增加。  |  Gu, Z., et al. 2000. Oncogene. 19: 1288-96. PMID: 10713670
  3. 膀胱, 食道和胃肿瘤中细胞表面抗原 LY-6 家族成员 PSCA 的表达减少。  |  Bahrenberg, G., et al. 2000. Biochem Biophys Res Commun. 275: 783-8. PMID: 10973799
  4. 前列腺干细胞抗原是有望用于晚期前列腺癌免疫疗法的候选抗原。  |  Dannull, J., et al. 2000. Cancer Res. 60: 5522-8. PMID: 11034097
  5. PSCA通过p38/NF-κB信号调节前列腺癌中IL-6的表达。  |  Liu, L., et al. 2017. Prostate. 77: 1389-1400. PMID: 28845520
  6. PSCA 的下调会促进胃癌的增殖，并与预后不良有关。  |  Xu, LP., et al. 2020. J Cancer. 11: 2708-2715. PMID: 32201541
  7. PSCA 作为新型癌症预后标志物和治疗靶点的意义。  |  Nayerpour Dizaj, T., et al. 2024. Cancer Cell Int. 24: 135. PMID: 38627732
  8. 人类抗 PSCA CAR 巨噬细胞对胰腺癌具有强大的抗肿瘤活性。  |  Shah, Z., et al. 2024. Cell Stem Cell. 31: 803-817.e6. PMID: 38663406
  9. PSCA-CAR T 细胞疗法治疗转移性去势抵抗性前列腺癌：一期试验。  |  Dorff, TB., et al. 2024. Nat Med. 30: 1636-1644. PMID: 38867077
  10. PSCA rs2976395 功能变异与胰腺癌风险之间的潜在关联。  |  Corradi, C., et al. 2024. Int J Cancer. 155: 1432-1442. PMID: 38924078
  11. 发现一种新型的高度特异性全人源 PSCA 抗体，并将其作为抗体药物共轭物应用于前列腺癌的治疗。  |  Chu, X., et al. 2024. MAbs. 16: 2387240. PMID: 39113562
  12. 前列腺干细胞抗原：一种在前列腺癌中过度表达的细胞表面标志物。  |  Reiter, RE., et al. 1998. Proc Natl Acad Sci U S A. 95: 1735-40. PMID: 9465086

订购信息

产品名称产品编号规格价格数量收藏夹

PSCA 抗体 (7F5)

sc-80654
200 µg/ml
RMB2377.00

PSCA (7F5): m-IgG Fc BP-HRP 套装

sc-527053
200 µg Ab; 10 µg BP
RMB2662.00

PSCA (7F5): m-IgG1 BP-HRP 套装

sc-532426
200 µg Ab; 20 µg BP
RMB2662.00

PSCA 抗体 (7F5) AC

sc-80654 AC
500 µg/ml, 25% agarose
RMB3129.00

PSCA 抗体 (7F5) HRP

sc-80654 HRP
200 µg/ml
RMB2377.00

PSCA 抗体 (7F5) FITC

sc-80654 FITC
200 µg/ml
RMB2482.00

PSCA 抗体 (7F5) PE

sc-80654 PE
200 µg/ml
RMB2580.00

PSCA 抗体 (7F5) Alexa Fluor® 488

sc-80654 AF488
200 µg/ml
RMB2685.00

PSCA 抗体 (7F5) Alexa Fluor® 546

sc-80654 AF546
200 µg/ml
RMB2685.00

PSCA 抗体 (7F5) Alexa Fluor® 594

sc-80654 AF594
200 µg/ml
RMB2685.00

PSCA 抗体 (7F5) Alexa Fluor® 647

sc-80654 AF647
200 µg/ml
RMB2685.00

PSCA 抗体 (7F5) Alexa Fluor® 680

sc-80654 AF680
200 µg/ml
RMB2685.00

PSCA 抗体 (7F5) Alexa Fluor® 790

sc-80654 AF790
200 µg/ml
RMB2685.00

What is the pH value of antigen repair in IF test.

Asked by: sang
Thank you for your question. The PH is related to the mode of antigen repair. You can refer to the following repair methods. Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Zhanglong
Date published: 2025-03-17

what is the stock conc for PSCA (7F5)sc-80654 lot #C1424

Asked by: Anonymous
Thank you for your question. The concentration is 200ug/1ml.
Answered by: BlakeJ
Date published: 2024-08-06

This antibody can be used for flowcytometry? If yes, then please let me know which fluorescent tag is present on this antibody?

Asked by: suman bishnoi
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-01-25

Do we have any flow cytometry data using this antibody?

Asked by: vish0005
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2022-10-03

Does this antibody work against mouse PSCA?

Asked by: JZ12345
Thank you for your question. We haven’t yet tested this antibody in mouse samples and so that is not a recommended species at this time. Please check back for updates!
Answered by: Tech Service
Date published: 2019-06-18

Is this antibody good for WB?

Asked by: CAJW
Thank you for your question. PSCA Antibody (7F5): sc-80654 is in fact recommended for detection of PSCA of human origin by WB, as well as IF, IHC(P) and FCM. There is published data for the use of this antibody in western blots as well (PMID 22133690).
Answered by: Tech Support Europe
Date published: 2019-06-17

Does the antibody only consists of the light chain of IgG? Can it be coupled to protein G coated magnetic beads?

Asked by: DGKCZ
Thank you for your question! No, the antibody consists of two heavy chains and two kappa light chains. Yes, you should be able to couple it with protein G coated magnetic beads, but we do not offer a protcool for this use. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-03-22

What is the exact epitope recognized by IPSCA (7F5): sc-80654 monoclonal antibody?

Asked by: cjMara
PSCA (7F5): sc-80654 is a mouse monoclonal raised against raised against P815 cells expressing PSCA of human origin. The exact epitope has not been determined.
Answered by: Technical Support 9
Date published: 2017-01-20
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_8
  • loc_zh_CN, sid_80654, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 116ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Superb IHCGreat product for IHC staining applications; strong signal
Date published: 2017-02-05
Rated 5 out of 5 by from Good choice for IHC(P)PSCA Antibody (7F5) is an excellent choice for IHC(P).
Date published: 2016-12-29
Rated 5 out of 5 by from GreatSeems good! Have to try! Should get an order in future.
Date published: 2016-12-02
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for IHC on human cancer cell - SCBT Publication Review
Date published: 2014-10-22
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_zh_CN, sid_80654, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
PSCA 抗体 (7F5) is rated 5.0 out of 5 by 4.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_zh_CN, sid_80654, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 200ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。