Date published: 2025-9-8

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PrP 抗体 (5B2): sc-47730

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说明书
  • PrP 抗体 5B2 是小鼠单克隆 IgG1 κ,PrP抗体, 在6篇文献中引用,规格为200 µg/ml
  • 针对全长重组小鼠PrP的抗原,其表位在N-terminus附近
  • PrP 抗体 (5B2) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA,检测mammalian 来源的 PrP
  • 抗PrP抗体(5B2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PrP (5B2): sc-47730免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 PrP 抗体 (5B2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PrP 抗体 (5B2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

PrP抗体(5B2)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)、流式细胞术(FCM)和酶联免疫吸附测定(ELISA)检测哺乳动物来源的朊病毒蛋白(PrP)。PrP (5B2) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。PrP在神经保护和细胞信号传导中发挥着关键作用,而PrP的错折叠是致命神经退行性疾病--朊病毒病的发病机制的核心。正常细胞形式的PrP(PrPc)主要位于大脑中,参与突触功能和神经保护。然而,当PrPc错误折叠成与疾病相关的形式(PrPSc)时,PrPSc会变得对蛋白水解降解具有抵抗力,导致有毒聚合物的积累,从而破坏细胞功能并最终导致神经退化。抗PrP抗体(5B2)可以区分这两种形式的PrP,因此这种抗体对于理解朊病毒疾病(如羊瘙痒症、牛海绵状脑病和克雅氏病)的机理至关重要,对于研究和诊断应用也很有价值。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

PrP 抗体 (5B2)

sc-47730
200 µg/ml
RMB2377.00

PrP (5B2): m-IgG Fc BP-HRP 套装

sc-528512
200 µg Ab; 10 µg BP
RMB2662.00

PrP (5B2): m-IgGκ BP-HRP 套装

sc-520907
200 µg Ab, 40 µg BP
RMB2662.00

PrP (5B2): m-IgG1 BP-HRP 套装

sc-542969
200 µg Ab; 20 µg BP
RMB2662.00

PrP 抗体 (5B2) AC

sc-47730 AC
500 µg/ml, 25% agarose
RMB3129.00

PrP 抗体 (5B2) HRP

sc-47730 HRP
200 µg/ml
RMB2377.00

PrP 抗体 (5B2) FITC

sc-47730 FITC
200 µg/ml
RMB2482.00

PrP 抗体 (5B2) PE

sc-47730 PE
200 µg/ml
RMB2580.00

PrP 抗体 (5B2) Alexa Fluor® 488

sc-47730 AF488
200 µg/ml
RMB2685.00

PrP 抗体 (5B2) Alexa Fluor® 546

sc-47730 AF546
200 µg/ml
RMB2685.00

PrP 抗体 (5B2) Alexa Fluor® 594

sc-47730 AF594
200 µg/ml
RMB2685.00

PrP 抗体 (5B2) Alexa Fluor® 647

sc-47730 AF647
200 µg/ml
RMB2685.00

PrP 抗体 (5B2) Alexa Fluor® 680

sc-47730 AF680
200 µg/ml
RMB2685.00

PrP 抗体 (5B2) Alexa Fluor® 790

sc-47730 AF790
200 µg/ml
RMB2685.00

Dear Santa Cruz Team, I would like to test the anti-PrP antibodies: FREE 10 µg sample of PrP (8B4): sc-47729. and FREE 10 µg sample of PrP (5B2): sc-47730. Is it possible to provide me with those? Thank you very much in advance.

Asked by: Ina Vorberg
Thank you for your interest in our PrP (8B4): sc-47729 and PrP (5B2): sc-47730. We would be happy to provide you with samples. Please contact our European Technical Service team by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com to arrange the samples.
Answered by: Technical Service
Date published: 2017-07-14

Can PrP (5B2): sc-47730 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PrP (5B2): sc-47730 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good for human tissueサンプルとして提供していただきました。ヒト検体で免疫染色、ウエスタンブロット共に良好でした。他のアプリケーションは試していません。
Date published: 2019-03-25
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of PrP expression in mouse brain tissue extract and U-87 MG and C32 whole cell lysates.
Date published: 2017-02-08
Rated 4 out of 5 by from WB publishable data with human and mouseWB publishable data with human and mouse brain cell - SCBT Publication Review
Date published: 2015-05-09
Rated 3 out of 5 by from Robust bands seen in WB using mouseRobust bands seen in WB using mouse, rat and human fetal brain tissue extracts. -SCBT QC
Date published: 2015-03-26
Rated 4 out of 5 by from Robust immunoperoxidase cytoplasmicRobust immunoperoxidase cytoplasmic, membrane and nuclear staining in formalin fixed, paraffin-embedded human cerebellum tissue. -SCBT QC
Date published: 2013-08-03
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PrP 抗体 (5B2) is rated 4.2 out of 5 by 5.
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