Date published: 2025-9-8
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Prohibitin 2 Antibody (A-2) - Western Blotting - Image 354866
Prohibitin 2 抗体 (A-2): sc-133094. BJAB (A), F9 (B), Ramos (C), A-431 (D), NIH/3T3 (E) 和 SK-BR-3 (F) 全细胞裂解液中抑制素 2 表达的 Western 印迹分析.
Prohibitin 2 抗体 (A-2): sc-133094. BJAB (A), F9 (B), Ramos (C), A-431 (D), NIH/3T3 (E) 和 SK-BR-3 (F) 全细胞裂解液中抑制素 2 表达的 Western 印迹分析.

Prohibitin 2 抗体 (A-2): sc-133094

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说明书
  • Prohibitin 2 抗体 A-2 是小鼠单克隆 IgG1 κ，Prohibitin 2抗体, 在30篇文献中引用，规格为200 µg/ml
  • 针对氨基酸220-299，在Prohibitin 2的human起源的C-terminus处进行映射
  • Prohibitin 2 抗体 (A-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Prohibitin 2
  • 抗Prohibitin 2抗体（A-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Prohibitin 2 (A-2): sc-133094的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Prohibitin 2 抗体 (A-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Prohibitin 2 抗体 (A-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Prohibitin抗体

Prohibitin 2抗体（A-2）是一种IgG1 κ小鼠单克隆Prohibitin 2抗体（也称为PHB2抗体、Prohibitin II型抗体、Prohibitin 2型抗体或线粒体PHB复合体组分2抗体），可通过WB, IP, IF, IHC(P)和ELISA检测小鼠、大鼠和人类来源的Prohibitin 2蛋白。Prohibitin 2抗体（A-2）既有非偶联形式的抗Prohibitin 2抗体，也有多种偶联形式的抗Prohibitin 2抗体，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。Prohibitin是一种具有抗增殖活性的进化保守蛋白。编码人类Prohibitin的基因位于17q21染色体上，且普遍表达。Prohibitin是一种合成后修饰的蛋白质，定位于线粒体内膜，通过阻断G1和S期之间的过渡来调节细胞周期，同时也定位于B细胞质膜，介导B细胞成熟。Prohibitin的mRNA和蛋白水平在G1期较高，在S期下降，在G2期再次上升，在M期下降，这表明Prohibitin通过转录和翻译后机制控制细胞周期。Prohibitin还是一种潜在的抑癌蛋白，可与视网膜母细胞瘤（Rb）结合，随后抑制特定信号级联反应中E2F家族成员的活性。Prohibitin 2是雌激素受体活性的抑制因子，是胚胎发育过程中幼虫性腺体细胞和生殖细胞分化所必需的。在分析的细胞系中，超过80%的细胞系中Prohibitin基因的突变与乳腺癌的发生和/或进展相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Prohibitin 2 抗体 (A-2) 参考文献:

  1. 抑制素是一种潜在的肿瘤抑制因子，它与 RB 相互作用并调节 E2F 的功能。  |  Wang, S., et al. 1999. Oncogene. 18: 3501-10. PMID: 10376528
  2. Rb 和禁止素针对 E2F1 的不同区域进行抑制，并对不同的上游信号做出反应。  |  Wang, S., et al. 1999. Mol Cell Biol. 19: 7447-60. PMID: 10523633
  3. 经植物醇酯处理的慢性白血病 B 淋巴细胞中抑制素的表达增加。  |  Woodlock, TJ., et al. 2001. Blood Cells Mol Dis. 27: 27-34. PMID: 11162143
  4. 在散发性乳腺癌中，位于染色体 17q21 上的人类禁止素基因发生了突变。  |  Sato, T., et al. 1992. Cancer Res. 52: 1643-6. PMID: 1540973
  5. 细胞周期活性和禁止素 mRNA 的表达。  |  Roskams, AJ., et al. 1993. J Cell Physiol. 157: 289-95. PMID: 8227162
  6. 抑制素：在衰老, 发育和肿瘤抑制中的潜在作用。  |  McClung, JK., et al. 1995. Exp Gerontol. 30: 99-124. PMID: 8591812
  7. 抑制素与衰老表型  |  Dell'Orco, RT., et al. 1996. Exp Gerontol. 31: 245-52. PMID: 8706794
  8. 乳腺癌细胞系中的抑制素：抗增殖活性的丧失与 3' 非翻译区突变有关。  |  Jupe, ER., et al. 1996. Cell Growth Differ. 7: 871-8. PMID: 8809404

订购信息

产品名称产品编号规格价格数量收藏夹

Prohibitin 2 抗体 (A-2)

sc-133094
200 µg/ml
RMB2377.00

Prohibitin 2 (A-2): m-IgG Fc BP-HRP 套装

sc-528747
200 µg Ab; 10 µg BP
RMB2662.00

Prohibitin 2 (A-2): m-IgGκ BP-HRP 套装

sc-521232
200 µg Ab, 40 µg BP
RMB2662.00

Prohibitin 2 (A-2): m-IgG1 BP-HRP 套装

sc-543109
200 µg Ab; 20 µg BP
RMB2662.00

Prohibitin 2 抗体 (A-2) AC

sc-133094 AC
500 µg/ml, 25% agarose
RMB3129.00

Prohibitin 2 抗体 (A-2) HRP

sc-133094 HRP
200 µg/ml
RMB2377.00

Prohibitin 2 抗体 (A-2) FITC

sc-133094 FITC
200 µg/ml
RMB2482.00

Prohibitin 2 抗体 (A-2) PE

sc-133094 PE
200 µg/ml
RMB2580.00

Prohibitin 2 抗体 (A-2) Alexa Fluor® 488

sc-133094 AF488
200 µg/ml
RMB2685.00

Prohibitin 2 抗体 (A-2) Alexa Fluor® 546

sc-133094 AF546
200 µg/ml
RMB2685.00

Prohibitin 2 抗体 (A-2) Alexa Fluor® 594

sc-133094 AF594
200 µg/ml
RMB2685.00

Prohibitin 2 抗体 (A-2) Alexa Fluor® 647

sc-133094 AF647
200 µg/ml
RMB2685.00

Prohibitin 2 抗体 (A-2) Alexa Fluor® 680

sc-133094 AF680
200 µg/ml
RMB2685.00

Prohibitin 2 抗体 (A-2) Alexa Fluor® 790

sc-133094 AF790
200 µg/ml
RMB2685.00

Can Prohibitin 2 (A-2): sc-133094 be used in ELISA with pure protein? Is there certificate of analysis data in this application?

Asked by: youngran
Thank you for your question. We haven’t tested this antibody by ELISA with protein, but it should be suitable for that application. We do not provide Certificates of Analysis for our antibodies.
Answered by: Technical Support
Date published: 2019-01-25

Can Prohibitin 2 (A-2): sc-133094 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Prohibitin 2 (A-2): sc-133094 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good for WBI detected a clear band around 36kDa in HeLa, HepG2, and mouse primary hepatocyte, although I have not checked whether it is specific band or not.
Date published: 2020-11-30
Rated 5 out of 5 by from Very GoodWorks superb on MEF cells and good for ICC and as well as for PLA
Date published: 2017-12-07
Rated 5 out of 5 by from Great featuresI got it a month ago, it works good for both WB and staining
Date published: 2017-10-30
Rated 5 out of 5 by from Western BlottingAntibody works nicely with a single band at 37 kDa
Date published: 2017-02-06
Rated 5 out of 5 by from ExcellentThis antibody works great in western blot analysis of Prohibitin 2 expression in BJAB, F9, Ramos, A-431, NIH/3T3 and SK-BR-3 whole cell lysates.
Date published: 2017-01-26
Rated 5 out of 5 by from WB results were greatWB results were great. Will be purchasing again.
Date published: 2015-10-20
Rated 5 out of 5 by from Used in Western BlotUsed in Western Blot.
Date published: 2015-06-08
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-05-27
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Prohibitin 2 抗体 (A-2) is rated 4.7 out of 5 by 12.
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