Date published: 2025-9-6
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pro BDNF 抗体 (5H8): sc-65514

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说明书
  • pro BDNF 抗体 5H8 是小鼠单克隆 IgG1 κ，pro BDNF抗体, 在82篇文献中引用，规格为200 µg/ml
  • 与human的104-112 PPLLFLEEv氨基酸残基的pro BDNF
  • pro BDNF 抗体 (5H8) 推荐用于 WB, IP 和 IF，检测mouse, rat, human, bovine, porcine 和canine 来源的 BDNF preproprotein
  • 抗pro BDNF抗体（5H8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取pro BDNF (5H8): sc-65514的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是pro BDNF Antibody (5H8) for WB applications. 的首选辅助检测试剂，这些试剂现与pro BDNF Antibody (5H8) 打包提供（请参阅下面的订购信息）。

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pro BDNF 抗体（5H8）是一种小鼠单克隆 IgG1 kappa 轻链抗体，设计用于检测脑源性神经营养因子（proBDNF）的前体形式，也称为 preproBDNF。抗 proBDNF 抗体 (5H8) 以人 proBDNF 的 104-112 氨基酸 (PPLLFLEEv) 内的一个精确表位为靶标，可确保在包括小鼠、大鼠、人、牛、猪和犬在内的多个物种中准确识别。proBDNF主要定位于神经元的分泌途径，如内质网和高尔基体，在这里加工成成熟的BDNF，BDNF是一种重要的神经营养素，参与神经元的存活、分化和突触可塑性。proBDNF单克隆抗体（5H8）可在免疫印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测中有效发挥作用，是研究神经营养素信号通路的研究人员不可或缺的工具。抗 proBDNF 抗体 (5H8) 既有非共轭形式，也有多种共轭形式，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 染料，可用于各种实验方案。proBDNF (5H8) 抗体能详细揭示 proBDNF 的细胞定位和处理过程，从而为旨在揭示神经元功能和大脑健康的复杂机制的关键研究提供支持。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

pro BDNF 抗体 (5H8) 参考文献:

  1. 脑源性神经营养因子前体的生物合成和翻译后加工。  |  Mowla, SJ., et al. 2001. J Biol Chem. 276: 12660-6. PMID: 11152678
  2. 神经营养素受体：神经元分化的窗口  |  Chao, MV. 1992. Neuron. 9: 583-93. PMID: 1327010
  3. 在发育中的视网膜网状系统中，BDNF 诱导的逆行突触快速改变。  |  Du, JL. and Poo, MM. 2004. Nature. 429: 878-83. PMID: 15215865
  4. 来自小胶质细胞的 BDNF 会导致神经元阴离子梯度发生变化，从而引发神经性疼痛。  |  Coull, JA., et al. 2005. Nature. 438: 1017-21. PMID: 16355225
  5. 神经营养因子的变化。  |  Thoenen, H. 1991. Trends Neurosci. 14: 165-70. PMID: 1713715
  6. BDNF的细胞生物学及其与精神分裂症的关系  |  Lu, B. and Martinowich, K. 2008. Novartis Found Symp. 289: 119-29; discussion 129-35, 193-5. PMID: 18497099
  7. 神经系统发育过程中的细胞死亡  |  Oppenheim, RW. 1991. Annu Rev Neurosci. 14: 453-501. PMID: 2031577
  8. 急性运动可调节免疫细胞中的BDNF和前BDNF蛋白含量  |  Brunelli, A., et al. 2012. Med Sci Sports Exerc. 44: 1871-80. PMID: 22543740
  9. 皮质GABA能神经元中BDNF的表达  |  Barreda Tomás, FJ., et al. 2020. Int J Mol Sci. 21: PMID: 32106593
  10. 神经营养素与神经细胞和非神经细胞中的 Trk 受体相互作用的异同。  |  Ip, NY., et al. 1993. Neuron. 10: 137-49. PMID: 7679912
  11. 神经营养素在小鼠神经元发育中的作用  |  Klein, R. 1994. FASEB J. 8: 738-44. PMID: 8050673
  12. 神经营养因子概念：重新审视。  |  Korsching, S. 1993. J Neurosci. 13: 2739-48. PMID: 8331370

订购信息

产品名称产品编号规格价格数量收藏夹

pro BDNF 抗体 (5H8)

sc-65514
200 µg/ml
RMB2377.00

pro BDNF (5H8): m-IgG Fc BP-HRP 套装

sc-526958
200 µg Ab; 10 µg BP
RMB2662.00

pro BDNF (5H8): m-IgG1 BP-HRP 套装

sc-532331
200 µg Ab; 20 µg BP
RMB2662.00

pro BDNF 抗体 (5H8) AC

sc-65514 AC
500 µg/ml, 25% agarose
RMB3129.00

pro BDNF 抗体 (5H8) HRP

sc-65514 HRP
200 µg/ml
RMB2377.00

pro BDNF 抗体 (5H8) FITC

sc-65514 FITC
200 µg/ml
RMB2482.00

pro BDNF 抗体 (5H8) PE

sc-65514 PE
200 µg/ml
RMB2580.00

pro BDNF 抗体 (5H8) Alexa Fluor® 488

sc-65514 AF488
200 µg/ml
RMB2685.00

pro BDNF 抗体 (5H8) Alexa Fluor® 546

sc-65514 AF546
200 µg/ml
RMB2685.00

pro BDNF 抗体 (5H8) Alexa Fluor® 594

sc-65514 AF594
200 µg/ml
RMB2685.00

pro BDNF 抗体 (5H8) Alexa Fluor® 647

sc-65514 AF647
200 µg/ml
RMB2685.00

pro BDNF 抗体 (5H8) Alexa Fluor® 680

sc-65514 AF680
200 µg/ml
RMB2685.00

pro BDNF 抗体 (5H8) Alexa Fluor® 790

sc-65514 AF790
200 µg/ml
RMB2685.00

QUE DIFERNECIA TIENE EL PRO, AL BDNF NORMAL, POLICLONAL?

Asked by: LOREN
Gracias por contactar a Santa Cruz Biotechnology. Para obtener asistencia, por favor comuníquese con el servicio técnico por correo electrónico a [email protected].
Answered by: BlakeJ
Date published: 2025-03-07

Is this antibody unconjugated?

Asked by: Canan
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

We want to detect mature BDNF in rat brain tissue using immunohistochemistry. Is there a specific BDNF antibody for this purpose? Thank you

Asked by: Despina
Thank you for your question. We currently only offer antibodies that are specific against pro BDNF.
Answered by: Tech Support Europe
Date published: 2021-03-03

Hello, I want to detect BDNF neurons in the mouse brain by IF. Do you recommend which product for this purpose, sc-65514 or sc-65513? Could you send me a sample for try? Thank you, Zhi

Asked by: zzrushui1
Thank you for your question. Both of these antibodies are recommended for detection of pro BDNF in mouse samples by IF. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of these monoclonal antibodies.
Answered by: Technical Support
Date published: 2019-06-16

What anti BDNF antibody to use for capturing mature BDNF using Immunoprecipitation? Previously (N-20): sc-546 antibody was used for the same but it has been discontinued.

Asked by: Trina
Thank you for your question. You can find our BDNF antibodies here on our website. Both are recommended for IP use.
Answered by: Tech Service
Date published: 2022-02-21

is it possible to do IHC on 4% PFA fixed mouse brain with this antibody? Or is the sc-65513 possible?

Asked by: Hyeijung
We have not yet tested either pro BDNF antibodies (sc-65514 and sc-65513) on formalin-fixed paraffin embedded tissue sections and so IHC(P) is not a recommended application at this time. Check back for updates!
Answered by: Technical Support
Date published: 2018-12-21

Can pro BDNF (5H8): sc-65514 monoclonal antibody be used for detecting mature BDNF？

Asked by: wangke
Thank you for your question. This antibody, pro BDNF (5H8): sc-65514, is not recommended for detection of mature BDNF. The antibody recognizes an epitope that is specific for detection of BDNF preproprotein.
Answered by: Technical Support
Date published: 2022-02-08

Does this antibody stains (IF) the same pattern as your old SC-546 in mouse? Thanks.

Asked by: Virginia66
Thank you for your question! Unfortunately we do not yet have IF data for this monclonal BDNF clone, so I can not answer your question. However, I can send you a sample vial of sc-65514, so you can give it a try free of charge. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2025-07-09
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Rated 2 out of 5 by from Rather so-soIn immunohistochemical staining (rat brain tissue fixed in formalin), the antibody produced rather disappointing results. Cell bodies were left unstained, and only cellular processes, not sure whether specifically, were stained. Numerous dilutions, procedures, and antigen retrieval techniques were examined, also taken from cited publications. Current rating: 2 out of 5, but there are still ongoing optimisation efforts.
Date published: 2024-12-03
Rated 5 out of 5 by from mouse anti-proBDNF antibody (sc-65514)I have performed immunohistochemistry at a 1:100 dilution of your mouse anti-proBDNF antibody (sc-65514) and have good images to include in the datasheet. The tissue is mouse brain (prefrontal cortex and dorsal hippocampus) and has been perfused in 4% paraformaldehyde and cut by vibratome at 40 microns of thickness. DAB has been used as a chromogen.
Date published: 2021-05-28
Rated 5 out of 5 by from Good antibody!1:100 diluted for wb of rat protein,the band is strong and special
Date published: 2019-03-28
Rated 1 out of 5 by from Scarso FunzionamentoL’anticorpo è stato testato mediante immunoistochimica ed immunofluorescenza (1:250) su campioni di encefalo di bovino fissati in formalina ed inclusi in paraffina. Le prove hanno mostrato segnale aspecifico in particolare a livello del giro dentato dell'ippocampo. Lo stesso anticorpo è stato testato anche mediante western blot (1:500) su omogenati di encefalo di bovino. Le prove hanno mostrato segnale aspecifico con bandeggio sopra i 50 KDa in tutte le aree cerebrali analizzate (ippocampo, corteccia frontale, c. temporale e cervelletto) e nessun segnale sotto i 50 KDa.
Date published: 2018-12-21
Rated 3 out of 5 by from Antibody reviewThis antibody some what worked for western blotting staining with mouse tissue.
Date published: 2018-11-16
Rated 2 out of 5 by from Antibody reviewDid not work well with IF with mouse tissue at 1:100 dilution.
Date published: 2018-06-14
Rated 1 out of 5 by from leider kein pos Signal in IHCsuper schnelle Lieferung des Testantikörpers, leider kein positives Signal in der Immunhistologie (Kryo) wie erhofft.
Date published: 2018-04-18
Rated 1 out of 5 by from Cattivo funzionamentoHo ordinato il campione di prova, che è arrivato subito, ma l'anticorpo non funziona con tecniche di IHC pur avendo seguito i protocolli standard.
Date published: 2018-03-07
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pro BDNF 抗体 (5H8) is rated 3.9 out of 5 by 31.
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