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PRMT5 Antibody (A-11) - Western Blotting - Image 355270
PRMT5 抗体 (A-11): sc-376937. Jurkat (A), HeLa (B), c4 (C), NIH/3T3 (D), PC-12 (E) 和 KNRK (F) 全细胞裂解液中 PRMT5 表达的 Western 印迹分析.
PRMT5 抗体 (A-11): sc-376937. Jurkat (A), HeLa (B), c4 (C), NIH/3T3 (D), PC-12 (E) 和 KNRK (F) 全细胞裂解液中 PRMT5 表达的 Western 印迹分析.

PRMT5 抗体 (A-11): sc-376937

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说明书
  • PRMT5 抗体 A-11 是小鼠单克隆 IgG1 κ，PRMT5抗体, 在43篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列338-637位于human物种的PRMT5的C-terminus
  • PRMT5 抗体 (A-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PRMT5; 也和以下物种反应，包括： 和 equine, bovine, porcine and canine
  • 抗PRMT5抗体（A-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PRMT5 (A-11): sc-376937的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成PRMT5 抗体 (A-11)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PRMT 抗体 包括 PRMT1, PRMT2, PRMT5, PRMT6和PRMT7

PRMT5抗体（A-11）是一种IgG1 κ小鼠单克隆PRMT5抗体，可通过WB、IP、IF、IHC（P）和ELISA检测到小鼠、大鼠和人类来源的PRMT5蛋白。PRMT5抗体（A-11）既可作为非偶联抗PRMT5抗体，也可作为多种偶联形式的抗PRMT5抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。剪剪接体的形成包括Sm蛋白以有序的方式组装到snRNA上。这一过程由存活的运动神经元（SMN）蛋白所介导，并通过将Sm蛋白中特定的精氨酸残基修饰为对称二甲基精氨酸（sDMAs）而增强。Sm蛋白的sDMA修饰是由甲基体催化的，甲基体是一个由II型甲基转移酶PRMT5（也被指定为JAK结合蛋白1（JBP1））、pICln和两个新因子组成的复合物。PRMT5通过其富含精氨酸和甘氨酸（RG）的结构域与Sm蛋白结合，而pICln则与Sm结构域结合。PRMT5是蛋白质精氨酸甲基转移酶（PRMT）家族的一个独特成员，主要定位于多种组织的细胞质中。PRMT5还与细胞周期蛋白E1启动子的转录起始位点区域特异性结合，因此参与转录和增殖的控制。编码人类PRMT5的基因定位于14号染色体q11区。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PRMT5 抗体 (A-11) 参考文献:

  1. 酵母蛋白 Skb1 和 Hsl7p 的人类同源物与 Jak 激酶相互作用，并具有蛋白甲基转移酶活性。  |  Pollack, BP., et al. 1999. J Biol Chem. 274: 31531-42. PMID: 10531356
  2. PRMT3 是精氨酸 N-甲基转移酶家族中的一个独特成员。通过锌指结构域实现底物特异性。  |  Frankel, A. and Clarke, S. 2000. J Biol Chem. 275: 32974-82. PMID: 10931850
  3. Prmt5 形成独特的同源异构体，是蛋白精氨酸甲基转移酶家族的成员。  |  Rho, J., et al. 2001. J Biol Chem. 276: 11393-401. PMID: 11152681
  4. PRMT5（Janus 激酶结合蛋白 1）催化蛋白质中对称二甲基精氨酸残基的形成。  |  Branscombe, TL., et al. 2001. J Biol Chem. 276: 32971-6. PMID: 11413150
  5. 甲基化组是一个包含 JBP1 和 pICln 的 20S 复合物，可产生二甲基精氨酸修饰的 Sm 蛋白。  |  Friesen, WJ., et al. 2001. Mol Cell Biol. 21: 8289-300. PMID: 11713266
  6. 含有 PRMT5 和推定 U snRNP 组装因子 pICln 的复合物对 Sm 蛋白的甲基化作用。  |  Meister, G., et al. 2001. Curr Biol. 11: 1990-4. PMID: 11747828
  7. II 型精氨酸甲基转移酶 PRMT5 对转录的负调控。  |  Fabbrizio, E., et al. 2002. EMBO Rep. 3: 641-5. PMID: 12101096
  8. 蛋白甲基转移酶 PRMT5 可选择性地阻断致癌基因 ras-p21 的有丝分裂信号转导。  |  Chie, L., et al. 2003. Ann Clin Lab Sci. 33: 200-7. PMID: 12817625
  9. 癌症中的 MTAP 基因缺失导致易受 MAT2A/PRMT5/RIOK1 轴靶向作用的影响。  |  Marjon, K., et al. 2016. Cell Rep. 15: 574-587. PMID: 27068473
  10. PRMT5干扰通过重编程T细胞介导的反应和调节PD-L1的表达来驱动宫颈癌的抗肿瘤免疫。  |  Jiang, Y., et al. 2021. Theranostics. 11: 9162-9176. PMID: 34522232
  11. PRMT5 对血管平滑肌细胞表型转换和新内膜形成的表观遗传调控。  |  Zhu, N., et al. 2023. Cardiovasc Res. 119: 2244-2255. PMID: 37486354
  12. PRMT5 介导的 FUBP1 甲基化加速了前列腺癌的进展。  |  Yan, W., et al. 2024. J Clin Invest. 134: PMID: 39146021

订购信息

产品名称产品编号规格价格数量收藏夹

PRMT5 抗体 (A-11)

sc-376937
200 µg/ml
RMB2377.00

PRMT5 抗体 (A-11) AC

sc-376937 AC
500 µg/ml, 25% agarose
RMB3129.00

PRMT5 抗体 (A-11) HRP

sc-376937 HRP
200 µg/ml
RMB2377.00

PRMT5 抗体 (A-11) FITC

sc-376937 FITC
200 µg/ml
RMB2482.00

PRMT5 抗体 (A-11) PE

sc-376937 PE
200 µg/ml
RMB2580.00

PRMT5 抗体 (A-11) Alexa Fluor® 488

sc-376937 AF488
200 µg/ml
RMB2685.00

PRMT5 抗体 (A-11) Alexa Fluor® 546

sc-376937 AF546
200 µg/ml
RMB2685.00

PRMT5 抗体 (A-11) Alexa Fluor® 594

sc-376937 AF594
200 µg/ml
RMB2685.00

PRMT5 抗体 (A-11) Alexa Fluor® 647

sc-376937 AF647
200 µg/ml
RMB2685.00

PRMT5 抗体 (A-11) Alexa Fluor® 680

sc-376937 AF680
200 µg/ml
RMB2685.00

PRMT5 抗体 (A-11) Alexa Fluor® 790

sc-376937 AF790
200 µg/ml
RMB2685.00

Do you have a stock of this antibody now?

Asked by: yuans
Thank you for your question. Please contact our Customer Care Department for current product availability.
Answered by: Technical Support
Date published: 2018-12-12

What is the total volume provided of PRMT5 (A-11):sc-376937 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 4
Date published: 2017-01-13

Can PRMT5 (A-11): sc-376937 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, PRMT5 (A-11): sc-376937 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 5 out of 5 by from The sc-376937 ab works very wellI prepared 10µg of whole cell lysates of HuH7 or PLC cells and run them on a 10% SDS-PAGE gel. Proteins were transferred from the gel into a nitrocellulose membrane and after blocking, membranes were incubated with the respective antibody rocking them in a shaker at 4 degree over night. The membranes were then washed and incubated with the appropriate secondary antibody and developed with the LI-COR Odyssey imager. The antibody against PRMT5 specifically recognized one single protein and the staining produced a band at the expected molecular weight.
Date published: 2024-01-23
Rated 5 out of 5 by from Good in WBA clear band around the target size are detected in HepG2, Huh1 and HEK293 cells, although specificity is not confirmed by knockdown.
Date published: 2023-02-21
Rated 4 out of 5 by from Good AntibodyWorked fine on whole cell lysates from Colorectal cancer cell lines (HCT116, Caco2, DLD1) after some optimisations. Dilution used for Western Blotting 1:250
Date published: 2023-01-24
Rated 5 out of 5 by from Great antibodyWe purchased it a month ago and it works very well in western blotting, IF and IP.
Date published: 2021-06-18
Rated 3 out of 5 by from it is fineI tested the muscle tissue for WB. It has the signal but it is not very good.
Date published: 2020-02-04
Rated 5 out of 5 by from Very good in WBProduces a single very clean band in WB of untransfected whole lysates, used in 1% milk or non-milk blocks at 1:1,000 to 1:4,000.
Date published: 2018-04-13
Rated 5 out of 5 by from Good antibody!I got a free sample two weeks ago and it worked well on my WB.
Date published: 2018-02-15
Rated 5 out of 5 by from Worked excellent!This antibody worked well at 1:1000 with clear background for the western blot assay. I will recommend this antibody to my colleague
Date published: 2018-01-18
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PRMT5 抗体 (A-11) is rated 4.9 out of 5 by 23.
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