Date published: 2025-9-8
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PPARγ Antibody (E-8) - Western Blotting - Image 397989
PPARγ 抗体 (E-8): sc-7273. HeLa (A), Jurkat (B), U-937 (C), THP-1 (D) 和 MDA-MB-231 (E) 全细胞裂解液中 PPARγ 表达的 Western 印迹分析. 所用检测试剂: m-IgG1 BP-HRP: sc-525408.
PPARγ 抗体 (E-8): sc-7273. HeLa (A), Jurkat (B), U-937 (C), THP-1 (D) 和 MDA-MB-231 (E) 全细胞裂解液中 PPARγ 表达的 Western 印迹分析. 所用检测试剂: m-IgG1 BP-HRP: sc-525408.

PPARγ 抗体 (E-8): sc-7273

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寫評論提問

说明书
  • PPAR gamma 抗体 E-8 是小鼠单克隆 IgG1 κ，PPARγ抗体, 在1,181篇文献中引用，规格为200 µg/ml
  • 特定于原点 human 的 486-505 氨基酸之间的表位映射，位于 C-terminus 的 PPARγ（与相应的鼠序列相同）
  • PPAR gamma 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PPARγ1 and PPARγ2
  • PPAR gamma抗体（E-8）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联生物素 用于WB, IHC(P) 以及 ELISA;偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-7273 X, 200 µg/0.1 ml)
  • 关于如何获取PPARγ (E-8): sc-7273的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 PPARγ 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PPARγ 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 PPAR 抗体 包括 PPAR α, PPAR β, PPAR γ和PPAR γ 2

PPARγ抗体（E-8）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源的PPARγ蛋白。PPARγ (E-8) 抗体有非结合型和多种结合型，包括琼脂糖、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合型。建议使用 PPARγ 单克隆抗体 (E-8) 检测 PPARγ1 和 PPARγ2 基因产物。PPARγ是核激素受体超家族的一员，在调节与胰岛素敏感性、脂肪细胞分化以及脂质代谢相关的基因表达方面发挥着关键作用。它与类维生素X受体（RXRs）形成异二聚体，从而调节关键代谢过程中的基因转录，影响肥胖、糖尿病和心血管疾病等病症的病理生理学。PPARγ调节炎症并影响巨噬细胞活性，这对于维持免疫稳态至关重要。PPARγ小鼠单克隆抗体（E-8）有助于研究代谢紊乱的影响以及与各种配体的相互作用，包括可激活转录活性的前列腺素和噻唑烷二酮。PPARγ2基因中的Pro12Ala多态性与转激活能力改变有关，可能影响对氧化低密度脂蛋白的免疫反应，与2型糖尿病和腹部肥胖有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PPARγ 抗体 (E-8) 参考文献:

  1. 血管平滑肌PPARγ的抗炎机制。  |  Mukohda, M. and Ozaki, H. 2021. J Smooth Muscle Res. 57: 1-7. PMID: 33658456
  2. PPARγ在代谢、免疫和癌症中的作用：统一而多样的作用机制。  |  Hernandez-Quiles, M., et al. 2021. Front Endocrinol (Lausanne). 12: 624112. PMID: 33716977
  3. 非酒精性脂肪肝中的PPAR-γ信号：发病机制和治疗靶点。  |  Chen, H., et al. 2023. Pharmacol Ther. 245: 108391. PMID: 36963510
  4. PPAR-γ通过促进糖酵解来调节人类T辅助9细胞的效应物功能。  |  Bertschi, NL., et al. 2023. Nat Commun. 14: 2471. PMID: 37120582
  5. 天然PPARγ激动剂在非酒精性脂肪肝（NAFLD）中的治疗作用。  |  Singh, S., et al. 2024. Tissue Barriers. 12: 2289830. PMID: 38050958
  6. 过氧化物酶体增殖激活受体（PPARs）的差异表达：成年大鼠 PPAR-α, -beta 和-gamma 的组织分布。  |  Braissant, O., et al. 1996. Endocrinology. 137: 354-66. PMID: 8536636
  7. 过氧化物酶体增殖激活受体α基因的表达受到压力的刺激，并遵循昼夜节律。  |  Lemberger, T., et al. 1996. J Biol Chem. 271: 1764-9. PMID: 8576180
  8. 多种 PPAR 异构体对脂肪生成的不同激活作用。  |  Brun, RP., et al. 1996. Genes Dev. 10: 974-84. PMID: 8608944
  9. 大鼠肝脏中过氧化物酶体增殖激活受体-α mRNA 的调控。  |  Sterchele, PF., et al. 1996. Arch Biochem Biophys. 326: 281-9. PMID: 8611035
  10. 孤儿核荷尔蒙受体 LXR alpha 与过氧化物酶体增殖激活受体相互作用，抑制过氧化物酶体增殖因子信号传导。  |  Miyata, KS., et al. 1996. J Biol Chem. 271: 9189-92. PMID: 8621574
  11. 甲状腺激素和过氧化物酶体增殖物激活受体在调节过氧化物酶体增殖物反应基因中的相互关系  |  Hunter, J., et al. 1996. Mol Cell Endocrinol. 116: 213-21. PMID: 8647322
  12. 过氧化物酶体增殖激活受体（PPAR）在小鼠结肠粘膜中的表达。  |  Mansén, A., et al. 1996. Biochem Biophys Res Commun. 222: 844-51. PMID: 8651933

订购信息

产品名称产品编号规格价格数量收藏夹

PPARγ 抗体 (E-8)

sc-7273
200 µg/ml
RMB2377.00

PPARγ (E-8): m-IgG Fc BP-HRP 套装

sc-526493
200 µg Ab; 10 µg BP
RMB2662.00

PPARγ (E-8): m-IgG1 BP-HRP 套装

sc-531866
200 µg Ab; 20 µg BP
RMB2662.00

PPARγ 抗体 (E-8) X

sc-7273 X
200 µg/0.1 ml
RMB2377.00

PPARγ 抗体 (E-8) AC

sc-7273 AC
500 µg/ml, 25% agarose
RMB3129.00

PPARγ 抗体 (E-8) FITC

sc-7273 FITC
200 µg/ml
RMB2482.00

PPARγ 抗体 (E-8) PE

sc-7273 PE
200 µg/ml
RMB2580.00

PPARγ 抗体 (E-8) Alexa Fluor® 488

sc-7273 AF488
200 µg/ml
RMB2685.00

PPARγ 抗体 (E-8) Alexa Fluor® 546

sc-7273 AF546
200 µg/ml
RMB2685.00

PPARγ 抗体 (E-8) Alexa Fluor® 594

sc-7273 AF594
200 µg/ml
RMB2685.00

PPARγ 抗体 (E-8) Alexa Fluor® 647

sc-7273 AF647
200 µg/ml
RMB2685.00

PPARγ 抗体 (E-8) Alexa Fluor® 680

sc-7273 AF680
200 µg/ml
RMB2685.00

PPARγ 抗体 (E-8) Alexa Fluor® 790

sc-7273 AF790
200 µg/ml
RMB2685.00

PPARγ 抗体 (E-8) B

sc-7273 B
200 µg/ml
RMB2407.00

PPARγ 抗体 (E-8) TRITC

sc-7273 TRITC
200 µg/ml
RMB2407.00

PPARγ 抗体 (E-8) Alexa Fluor® 405

sc-7273 AF405
200 µg/ml
RMB2685.00

PPARγ (E-8) 阻断肽

sc-7273 P
100 µg/0.5 ml
RMB511.00

请问IP时一般稀释浓度是多少？

Asked by: Caicz
感谢您的提问。请参考官网IP的实验流程，https://www.scbt.com/zh/resources/protocols/immunoprecipitation-western-blots. 如果有其他问题，请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持” Thank you for the question. Please refer to the online protocol: https://www.scbt.com/zh/resources/protocols/immunoprecipitation-western-blots. If you have any other question, please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Sen Li
Date published: 2024-10-08

请问PPARγ 抗体 (E-8): sc-7273是什么来源的呢？如果要做小鼠小肠切片的免疫荧光染色需要用什么来源的二抗呢？推荐的稀释浓度是多少呢？

Asked by: Huang
感谢您的提问，PPAR gamma 抗体 E-8 是小鼠单克隆 IgG1 κ。二抗请选用其他种属抗小鼠。稀释比例为1:50-1:500。谢谢。 Thank you for your question, PPAR gamma antibody E-8 is a mouse monoclonal IgG1 κ. For secondary antibody, please use an anti-mouse of another species. The dilution ratio is 1:50-1:500. Thank you.
Answered by: Jiajun Z
Date published: 2024-07-22

Does this product react with the human placenta?

Asked by: Kamila
Thank you for your question. We have not tested the antibody for human placenta but we do warranty the antibody for mouse, rat and human samples . We offer a 1 year warranty from date of purchase .
Answered by: BlakeJ
Date published: 2023-11-30

Does HEK293 cells express this protein or could HEK293 cells be stimulated to express it?

Asked by: yuner
Thank you for your question. Unfortunately we are unable to answer the expression level in HEK293 cells specifically, but this information is probably available in literature, so I would recommend looking in pubmed. We do have data in many cell lines, and we do see PPARγ expression without stimulation in most cases.
Answered by: Tech Service
Date published: 2019-08-13

The bands we are obtaining in a WB of PAG with this antibody are around 45kDa, really close to the actin band. I am using a gel concentration of 8%. Is this band that I am obtaining PPAR gamma?? Is there any publication that can prove me it is OK?

Asked by: Biozeros
Thank you for your question. Yes, PPARgamma has been reported at several different sizes, ranging down to 43kDa and up to 67kDa. I would suggest not using actin as a loading control when you are working with PPARgamma. GAPDH or Vinculin may be better so as to not be in the same range as your target protein.
Answered by: Tech Service
Date published: 2024-11-06

hi, what blocking buffer that i should use with this antibody? is it better with BSA or non-fat milk?

Asked by: hsn14
Thank you for your question. For Western blotting with chemiluminescent detection (HRP), we recommend using 5% nonfat milk + TBS + 0.05% Tween-20 (available pre-made as, TBS Blotto A: sc-2333). For Western blotting with near-infrared (NIR) detection, we recommend using our UltraCruz® Blocking Reagent: sc-516214. A link to our recommended protocol for Western blotting is below as a reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-07-26

Can I use PPARγ Antibody (E-8) for Immunohistochemistry (IHC) staining on mouse tissue?

Asked by: Dr Ninau Qelp
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. Our PPARγ Antibody (E-8) is currently available conjugated to AF405, AF488, AF647, Biotin, FITC, HRP, PE, and TRITC.
Answered by: Tech Service 2
Date published: 2022-11-15

What is the recommended secondary reagent to be used with PPARγ Antibody (E-8)? 

Asked by: Dr Ninau Qelp
For Western blot we recommend our Mouse IgG Binding: m-IgGκ BP-HRP: sc-516102
Answered by: Tech Service 2
Date published: 2016-12-14
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Rated 5 out of 5 by from anti-PPARG works for western blotanti-PPARG antibody works best for western blots at 1:1000 dilution
Date published: 2024-04-05
Rated 4 out of 5 by from Good antibodyMultiple bands were detected but still expected size band was detected.
Date published: 2021-12-03
Rated 5 out of 5 by from 抗体很好用选择了该抗体，实验很顺利，很好显。该抗体在动物肝脏组织上面，做出了很好的效果，希望我的脂代谢方面的蛋白能顺利获取。
Date published: 2021-04-14
Rated 1 out of 5 by from l'anticorpo non funziona.l'anticorpo non funziona. Abbiamo provato ad usare diverse diluizioni, cambiare anticorpo secondario e soluzioni di sviluppo ma l'anticorpo non ha mai funzionato
Date published: 2020-11-06
Rated 5 out of 5 by from PPARgama in the VATThis antibody worked well in nuclear extracts of the visceral adipose tissue of mice.
Date published: 2019-12-10
Rated 3 out of 5 by from great antibodyi tested this antibody in wb in dilution 1/250 in N2A cells. The bands were detected were good.
Date published: 2019-04-01
Rated 5 out of 5 by from Remarkable featuresThis antibody worked well for IP、 WB and IF, published with human Human pulmonary alveolar epithelial cells.
Date published: 2019-03-29
Rated 2 out of 5 by from OKI bought this few months ago, it did not perform really clear band in western blot
Date published: 2018-09-28
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PPARγ 抗体 (E-8) is rated 4.4 out of 5 by 64.
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