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PP1β Antibody (A-6) - Western Blotting - Image 143551
PP1β (A-6): sc-365678. A-673 (A), SJRH30 (B), HeLa (C) 和 L8 (D) 全细胞裂解液中 PP1β 表达的 Western 印迹分析.
PP1β (A-6): sc-365678. A-673 (A), SJRH30 (B), HeLa (C) 和 L8 (D) 全细胞裂解液中 PP1β 表达的 Western 印迹分析.

PP1β 抗体 (A-6): sc-365678

5.0(6)
寫評論提問

说明书
  • PP1 beta 抗体 A-6 是小鼠单克隆 IgG2b κ，PP1β抗体, 在11篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的PP1β的C-terminus的氨基酸295-322之间
  • PP1 beta 抗体 (A-6) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的PP1β; 也和以下物种反应，包括： 和 canine, bovine, porcine and avian
  • 抗PP1 beta抗体（A-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PP1β (A-6): sc-365678的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是PP1β Antibody (A-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PP1β Antibody (A-6) 打包提供（请参阅下面的订购信息）。

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说明书与实验方案
研究信息

関連項目

额外PP1抗体，包括PP1 β

PP1β抗体（A-6）是一种IgG2b κ小鼠单克隆PP1β抗体（也称为PP1β抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的PP1β蛋白。PP1β抗体（A-6）有非偶联的抗PP1β抗体形式，以及多种偶联形式的抗PP1β抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在真核生物中，丝氨酸和苏氨酸残基上蛋白质的磷酸化和去磷酸化是调节广泛细胞功能的重要手段，包括分裂、稳态和凋亡。一组密切参与此过程的蛋白质是蛋白质磷酸酶。一般来说，蛋白质磷酸酶（PP）全酶是由调节亚基、可变亚基和催化亚基组成的三聚体复合物。已经鉴定出四种主要的蛋白质磷酸酶催化亚基家族，分别命名为PP1、PP2A、PP2B（钙调神经磷酸酶）和PP2C。另一种蛋白质磷酸酶催化亚基PPX（也称为PP4）是新型PP家族的假定成员。PP1家族由亚家族成员PP1α、PP1β和PP1γ组成，它们是MgATP依赖性酶。PP1的失活是通过其与抑制性蛋白NIPP-1（PP1的核抑制剂）的结合来维持的。NIPP-1被cAMP-PK或酪蛋白激酶II磷酸化后，会释放出活性的PP1。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

PP1β 抗体 (A-6)

sc-365678
200 µg/ml
RMB2377.00

PP1β (A-6): m-IgG Fc BP-HRP 套装

sc-529436
200 µg Ab; 10 µg BP
RMB2662.00

PP1β (A-6): m-IgGκ BP-HRP 套装

sc-522324
200 µg Ab, 40 µg BP
RMB2662.00

PP1β (A-6): m-IgG2b BP-HRP 套装

sc-550110
200 µg Ab; 10 µg BP
RMB2662.00

PP1β 抗体 (A-6) AC

sc-365678 AC
500 µg/ml, 25% agarose
RMB3129.00

PP1β 抗体 (A-6) HRP

sc-365678 HRP
200 µg/ml
RMB2377.00

PP1β 抗体 (A-6) FITC

sc-365678 FITC
200 µg/ml
RMB2482.00

PP1β 抗体 (A-6) PE

sc-365678 PE
200 µg/ml
RMB2580.00

PP1β 抗体 (A-6) Alexa Fluor® 488

sc-365678 AF488
200 µg/ml
RMB2685.00

PP1β 抗体 (A-6) Alexa Fluor® 546

sc-365678 AF546
200 µg/ml
RMB2685.00

PP1β 抗体 (A-6) Alexa Fluor® 594

sc-365678 AF594
200 µg/ml
RMB2685.00

PP1β 抗体 (A-6) Alexa Fluor® 647

sc-365678 AF647
200 µg/ml
RMB2685.00

PP1β 抗体 (A-6) Alexa Fluor® 680

sc-365678 AF680
200 µg/ml
RMB2685.00

PP1β 抗体 (A-6) Alexa Fluor® 790

sc-365678 AF790
200 µg/ml
RMB2685.00

PP1β (A-6) 中和勝肽

sc-365678 P
100 µg/0.5 ml
RMB511.00

Does this antibody recognize the alpha or gamma isoforms of PP1?

Asked by: MarkA
Thank you for your question. sc-365678 does not detect the alpha or gamma isoforms.
Answered by: BlakeJ
Date published: 2023-03-29

What application is the blocking peptide sc-365678 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good for WB and IPworked very good and showed specificity in WB (x500, RT 1hr, HeLa S3), IP (~1 µg)
Date published: 2022-08-07
Rated 5 out of 5 by from One of the best antibodies for PP1bIt detects endogenous PP1b in HEK293, A549 and RAW264.7 cells. A single strong and right size band was detected.
Date published: 2017-11-30
Rated 5 out of 5 by from Great for WesternIt works great for Western blot using K562 whole cell lysate!
Date published: 2017-07-06
Rated 5 out of 5 by from Excellent antibody for Western blotting.This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It very strongly detects an appropriately sized 37 kDa target protein in immunoblots.
Date published: 2016-10-13
Rated 5 out of 5 by from Produced superb immunofluorescence membraneProduced superb immunofluorescence membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-05-11
Rated 5 out of 5 by from Satisfactory Western Blot data of PP1 expressionSatisfactory Western Blot data of PP1 expression in A-673, SJRH30, HeLa and L8 whole cell lysates. -SCBT QC
Date published: 2013-11-17
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PP1β 抗体 (A-6) is rated 5.0 out of 5 by 6.
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