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Polyoma virus middle T antigen 抗体 (PyMT): sc-53481

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说明书
  • Polyoma virus middle T antigen 抗体 PyMT 是大鼠单克隆 IgG2b, 在19篇文献中引用,提供 200 µg/ml
  • 小鼠纤维母细胞瘤病毒转化细胞系REWA5/T1A1
  • 建议使用Polyoma virus middle T antigen抗体(PyMT)通过IP和IF检测Polyoma Virus
  • 抗Polyoma virus middle T antigen抗体(PyMT)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Polyoma virus middle T antigen (PyMT): sc-53481免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Polyoma virus middle T antigen 抗体 (PyMT)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

多瘤病毒中间T抗原抗体(PyMT)是一种鼠单克隆IgG2b抗体,可通过免疫沉淀(IP)和免疫荧光(IF)检测多瘤病毒来源的多瘤病毒中间T抗原。抗多瘤病毒中间T抗原抗体(PyMT)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。多瘤病毒是一种小型致癌DNA病毒,属于多瘤病毒科,以在感染宿主中诱发多种肿瘤而闻名。中间T抗原由421个氨基酸组成,是一种关键的转化蛋白,在细胞转化中起着重要作用,是转化过程必不可少的。中间T抗原至少分为三个功能域,其中一些与大T抗原和小T抗原域重叠,这突出了病毒生命周期中的复杂相互作用。中T抗原激活多种信号通路,促进细胞增殖和存活,因此这种蛋白质在肿瘤发生中起着关键作用。了解中T抗原的功能对于开发病毒引起的癌症的靶向治疗和干预措施至关重要,因为这项研究为肿瘤发生机制和潜在治疗靶标提供了见解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Polyoma virus middle T antigen 抗体 (PyMT) 参考文献:

  1. 多瘤病毒中间T抗原及其在识别癌症相关分子中的作用。  |  Dilworth, SM. 2002. Nat Rev Cancer. 2: 951-6. PMID: 12459733
  2. 多瘤病毒中间T抗原的转化机制。  |  Kaplan, DR., et al. 1989. Biochim Biophys Acta. 948: 345-64. PMID: 2465782
  3. 从重组杆状病毒中表达具有生物活性的多瘤病毒中间 T 抗原。  |  Forstová, J., et al. 1989. Nucleic Acids Res. 17: 1427-43. PMID: 2646594
  4. 多瘤早期基因产品在大肠杆菌中的表达。  |  Schaffhausen, B., et al. 1985. Nucleic Acids Res. 13: 501-19. PMID: 2987799
  5. 猿猴病毒 40 和多瘤病毒的大 T 抗原可有效建立原代成纤维细胞。  |  Jat, PS. and Sharp, PA. 1986. J Virol. 59: 746-50. PMID: 3016337
  6. 多瘤病毒小 T 抗原可增强病毒基因组在 3T6 小鼠成纤维细胞中的复制。  |  Berger, H. and Wintersberger, E. 1986. J Virol. 60: 768-70. PMID: 3022009
  7. 多瘤病毒大 T 抗原与人类 hsp70 启动子的结合不是反式激活所必需的。  |  Kingston, RE., et al. 1986. Mol Cell Biol. 6: 3180-90. PMID: 3023966
  8. 针对多瘤病毒肿瘤抗原的单克隆抗体。  |  Dilworth, SM. and Griffin, BE. 1982. Proc Natl Acad Sci U S A. 79: 1059-63. PMID: 6175960
  9. 多瘤病毒中间T抗原和pp60c-src的复合物。  |  Courtneidge, SA. and Smith, AE. 1984. EMBO J. 3: 585-91. PMID: 6325177
  10. 与多瘤病毒中间 T 抗原不同抗原形式相关的蛋白激酶作用。  |  Dilworth, SM. 1982. EMBO J. 1: 1319-28. PMID: 6327264
  11. 多瘤病毒 T 抗原干扰干扰素诱导基因的表达。  |  Weihua, X., et al. 1998. Proc Natl Acad Sci U S A. 95: 1085-90. PMID: 9448289
  12. 多瘤病毒大 T 抗原与转录辅激活蛋白 p300 结合。  |  Nemethova, M. and Wintersberger, E. 1999. J Virol. 73: 1734-9. PMID: 9882390

订购信息

产品名称产品编号规格价格数量收藏夹

Polyoma virus middle T antigen 抗体 (PyMT)

sc-53481
200 µg/ml
RMB2377.00

Polyoma virus middle T antigen 抗体 (PyMT) AC

sc-53481 AC
500 µg/ml, 25% agarose
RMB3129.00

Polyoma virus middle T antigen 抗体 (PyMT) HRP

sc-53481 HRP
200 µg/ml
RMB2377.00

Polyoma virus middle T antigen 抗体 (PyMT) FITC

sc-53481 FITC
200 µg/ml
RMB2482.00

Polyoma virus middle T antigen 抗体 (PyMT) PE

sc-53481 PE
200 µg/ml
RMB2580.00

Polyoma virus middle T antigen 抗体 (PyMT) Alexa Fluor® 488

sc-53481 AF488
200 µg/ml
RMB2685.00

Polyoma virus middle T antigen 抗体 (PyMT) Alexa Fluor® 546

sc-53481 AF546
200 µg/ml
RMB2685.00

Polyoma virus middle T antigen 抗体 (PyMT) Alexa Fluor® 594

sc-53481 AF594
200 µg/ml
RMB2685.00

Polyoma virus middle T antigen 抗体 (PyMT) Alexa Fluor® 647

sc-53481 AF647
200 µg/ml
RMB2685.00

Polyoma virus middle T antigen 抗体 (PyMT) Alexa Fluor® 680

sc-53481 AF680
200 µg/ml
RMB2685.00

Polyoma virus middle T antigen 抗体 (PyMT) Alexa Fluor® 790

sc-53481 AF790
200 µg/ml
RMB2685.00

Hi, How can we obtain the free 10 ug sample to test if this antibody will work for us? Thanks for your time.

Asked by: climatechange
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

What is the exact epitope recognized by Polyoma virus middle T antigen (PyMT): sc-53481 monoclonal antibody?

Asked by: cjMara
Polyoma virus middle T antigen (PyMT): sc-53481 is a mouse monoclonal raised against Polyoma virus-transformed Wistar rat fibroblast cell line REWA5/T1A1. The exact epitope has not been determined.
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 3 out of 5 by from OK for IHCUsed in IHC (frozen sections) - specific but weak signal, some background
Date published: 2018-02-01
Rated 4 out of 5 by from good antibodyI purchased this product to detect disseminating tumour cells in mice. The signal is good and specific,
Date published: 2017-11-23
Rated 5 out of 5 by from Cited antibodyThis monoclonal has been cited in several publications!
Date published: 2017-01-16
Rated 4 out of 5 by from The antibody worked for IF assay in mouse sampleThe antibody worked for IF assay in mouse sample.
Date published: 2015-06-26
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Polyoma virus middle T antigen 抗体 (PyMT) is rated 4.0 out of 5 by 5.
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