Date published: 2025-9-8

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POLA2 抗体 (D-2): sc-398255

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说明书
  • POLA2 抗体 D-2 是小鼠单克隆 IgG1 κ,POLA2抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列121-420位于human物种的POLA2的内部区域内
  • POLA2 抗体 (D-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 POLA2
  • 抗POLA2抗体(D-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取POLA2 (D-2): sc-398255免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是POLA2 Antibody (D-2) for WB applications. 的首选辅助检测试剂,这些试剂现与POLA2 Antibody (D-2) 打包提供(请参阅下面的订购信息)。

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関連項目

POLA2抗体(D-2)是一种IgG1 κ小鼠单克隆POLA2抗体(也被指定为POLA2抗体),它可以通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的POLA2蛋白。POLA2抗体(D-2)既可以非偶联的抗POLA2抗体形式提供,也可以多种偶联形式的抗POLA2抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。DNA聚合酶α是一种由4个亚基组成的酶复合物:DNA引发酶大亚基、DNA引发酶小亚基以及DNA聚合酶A和B亚基。该复合物在细胞周期中组装,是DNA复制的重要组成部分。POLA2,也被称为DNA聚合酶α B亚基,是DNA聚合酶α家族蛋白中的一个由598个氨基酸组成的成员。POLA2通过其250个氨基酸的N端结构域整合到4亚基酶复合物中。在染色体DNA复制的早期阶段,POLA2将引发酶/聚合酶复合物与复制机制偶联起来。POLA2定位于细胞核,并可能在细胞周期的G2/M期被磷酸化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

POLA2 抗体 (D-2) 参考文献:

  1. E2F介导了由Sp1控制的人类DNA聚合酶epsilon B亚基基因POLE2启动子的诱导。  |  Huang, D., et al. 2001. Nucleic Acids Res. 29: 2810-21. PMID: 11433027
  2. DNA 引物酶是 DNA 复制过程中的分子制动器。  |  Lee, JB., et al. 2006. Nature. 439: 621-4. PMID: 16452983
  3. 单链 DNA 在将 REV1 靶向引物末端方面的作用。  |  Masuda, Y. and Kamiya, K. 2006. J Biol Chem. 281: 24314-21. PMID: 16803901
  4. 人类 GINS 复合物能与人类 DNA 聚合酶 alpha-primase 结合,并对其产生特异性刺激。  |  De Falco, M., et al. 2007. EMBO Rep. 8: 99-103. PMID: 17170760
  5. 高等植物拟南芥和水稻核心 DNA 复制机制的全基因组分析。  |  Shultz, RW., et al. 2007. Plant Physiol. 144: 1697-714. PMID: 17556508
  6. 嗜卤古细菌模型 NRC-1 中的必要和非必要 DNA 复制基因。  |  Berquist, BR., et al. 2007. BMC Genet. 8: 31. PMID: 17559652

订购信息

产品名称产品编号规格价格数量收藏夹

POLA2 抗体 (D-2)

sc-398255
200 µg/ml
RMB2377.00

POLA2 (D-2): m-IgG Fc BP-HRP 套装

sc-527757
200 µg Ab; 10 µg BP
RMB2662.00

POLA2 (D-2): m-IgG1 BP-HRP 套装

sc-533130
200 µg Ab; 20 µg BP
RMB2662.00

POLA2 抗体 (D-2) AC

sc-398255 AC
500 µg/ml, 25% agarose
RMB3129.00

POLA2 抗体 (D-2) HRP

sc-398255 HRP
200 µg/ml
RMB2377.00

POLA2 抗体 (D-2) FITC

sc-398255 FITC
200 µg/ml
RMB2482.00

POLA2 抗体 (D-2) PE

sc-398255 PE
200 µg/ml
RMB2580.00

POLA2 抗体 (D-2) Alexa Fluor® 488

sc-398255 AF488
200 µg/ml
RMB2685.00

POLA2 抗体 (D-2) Alexa Fluor® 546

sc-398255 AF546
200 µg/ml
RMB2685.00

POLA2 抗体 (D-2) Alexa Fluor® 594

sc-398255 AF594
200 µg/ml
RMB2685.00

POLA2 抗体 (D-2) Alexa Fluor® 647

sc-398255 AF647
200 µg/ml
RMB2685.00

POLA2 抗体 (D-2) Alexa Fluor® 680

sc-398255 AF680
200 µg/ml
RMB2685.00

POLA2 抗体 (D-2) Alexa Fluor® 790

sc-398255 AF790
200 µg/ml
RMB2685.00

You say the second band is due to the fact there are two isoforms. One isoform is 66 KDa, the other around 40 KDa. I would expect a band running faster in the gel. Right?

Asked by: Vicky
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service. Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-03-13

Any comments on the double band ? Thanks

Asked by: Vicky
Thank you for your question. DNA polymerase alpha subunit B has a truncated alterntive splicing isoform that contains the epitope sequence the antibody targets, which is why the antibody detects both isoforms in western blots.
Answered by: Tech Support Europe
Date published: 2025-02-17

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with POLA2 (D-2): sc-398255?

Asked by: jenniferc
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from GreatThe antibody is pretty good for IF staining. The background is very low.
Date published: 2017-08-02
Rated 5 out of 5 by from Very good Western blot data of POLA2 expressionVery good Western blot data of POLA2 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2014-05-16
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POLA2 抗体 (D-2) is rated 5.0 out of 5 by 2.
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