Date published: 2025-9-8
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Podocalyxin-like 1 Antibody (3D3) - Western Blotting - Image 8610
PCLP1 (3D3): sc-23904. Ecv304 (A), H9 (B), Raji (C) 和 MIA PaCa-2 (D) 全细胞裂解液中 PCLP1 表达的 Western 印迹分析.
PCLP1 (3D3): sc-23904. Ecv304 (A), H9 (B), Raji (C) 和 MIA PaCa-2 (D) 全细胞裂解液中 PCLP1 表达的 Western 印迹分析.

Podocalyxin-like 1 抗体 (3D3): sc-23904

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说明书
  • Podocalyxin-like 1 抗体 3D3 是小鼠单克隆 IgG1 κ，Podocalyxin-like 1抗体, 在29篇文献中引用，规格为200 µg/ml
  • 针对human重组荚膜蛋白样蛋白
  • Podocalyxin-like 1 抗体 (3D3) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 Podocalyxin-like 1
  • 抗Podocalyxin-like 1抗体（3D3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Podocalyxin-like 1 (3D3): sc-23904的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Podocalyxin-like 1 抗体 (3D3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Podocalyxin-like 1 抗体 (3D3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Podocalyxin-like 1 Antibody (3D3) 是一种小鼠单克隆IgG1 kappa轻链抗体，针对人类重组类足钙蛋白样蛋白，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）特异性检测人类来源的类足钙蛋白样蛋白1（PODXL）。PODXL又名PCLP1或podocalyxin，是一种跨膜唾液酸蛋白，具有广泛的翻译后修饰，包括其胞外结构域的N-和O-连接糖基化和唾液酸化。这些修饰赋予其强大的负电荷，有助于PODXL的抗粘附特性，并维持肾小球滤过屏障中必不可少的足突。PODXL的细胞内结构域包含多个磷酸化位点，在细胞内信号传导通路中发挥重要作用，调节细胞粘附、迁移和极性。除肾脏外，PODXL在各种上皮组织中表达，包括乳腺、胰腺和前列腺，以及内皮细胞和造血干细胞的前体--血管母细胞。PODXL参与癌症的进展和转移，尤其是乳腺癌和前列腺癌，凸显其在肿瘤学中的临床意义。PODXL通过与维持细胞形状和极性至关重要的ezrin结合，与肌动蛋白细胞骨架相互作用，并作为L-选择素的配体，在炎症反应期间促进白细胞在内皮细胞上滚动。在结构上与CD34相关，PODXL在炎症过程中介导白细胞与内皮细胞之间的相互作用。抗PODXL抗体（3D3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor® 结合物、抗PODXL抗体（3D3）是研究人员研究PODXL在生理和病理条件下的作用的多功能工具，例如癌症进展和转移，因为其影响细胞粘附和迁移。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Podocalyxin-like 1 抗体 (3D3) 参考文献:

  1. 鉴定荚膜萼蛋白样蛋白 1 作为小鼠主动脉-性腺-肾小管区域血管母细胞的新型细胞表面标记物。  |  Hara, T., et al. 1999. Immunity. 11: 567-78. PMID: 10591182
  2. 鉴定内聚糖，CD34/podocalyxin sialomucins 家族的成员。  |  Sassetti, C., et al. 2000. J Biol Chem. 275: 9001-10. PMID: 10722749
  3. 原发性肝肿瘤和类肿瘤病变中的类足钙素样蛋白1表达。  |  Heukamp, LC., et al. 2006. Histopathology. 49: 242-7. PMID: 16918970
  4. Podocalyxin 样蛋白在多形性胶质母细胞瘤干样细胞中表达，并与不良预后有关。  |  Binder, ZA., et al. 2013. PLoS One. 8: e75945. PMID: 24146797
  5. 尿道膀胱癌的预后生物标志物--Podocalyxin样蛋白和RNA结合基序蛋白3：一项验证性研究。  |  Boman, K., et al. 2017. Biomark Res. 5: 10. PMID: 28293425
  6. 类足钙素样蛋白在各种癌症中的预后作用：系统回顾和荟萃分析。  |  Wang, J., et al. 2017. Oncotarget. 8: 52457-52464. PMID: 28881743
  7. 鉴定 LEA（一种荚膜萼蛋白样糖蛋白）作为结直肠癌进展的预测因子。  |  Yuan, D., et al. 2018. Cancer Med. 7: 5155-5166. PMID: 30277651
  8. 可切除胃癌和食管腺癌新辅助化疗获益的预测性生物标志物--鬼臼毒素样蛋白  |  Borg, D., et al. 2018. J Transl Med. 16: 290. PMID: 30355278
  9. 作为肾小球荚膜细胞和血管内皮细胞跨膜蛋白的兔荚膜alyxin 样蛋白 1 的分子克隆, 表达和表征。  |  Kershaw, DB., et al. 1995. J Biol Chem. 270: 29439-46. PMID: 7493982
  10. 兔和人炎症性肾小球疾病（新月体肾炎）中的肾小球上皮细胞蛋白 1 和荚膜alyxin 样蛋白 1。  |  Yang, DH., et al. 1996. Lab Invest. 74: 571-84. PMID: 8600307
  11. 选择素的 Sialomucin 配体：一个新的细胞粘附分子家族。  |  Lasky, LA. 1994. Princess Takamatsu Symp. 24: 81-90. PMID: 8983065
  12. 鉴定荚膜萼蛋白样蛋白为 L-选择素的高内皮静脉配体：与 CD34 相似。  |  Sassetti, C., et al. 1998. J Exp Med. 187: 1965-75. PMID: 9625756

订购信息

产品名称产品编号规格价格数量收藏夹

Podocalyxin-like 1 抗体 (3D3)

sc-23904
200 µg/ml
RMB2377.00

Podocalyxin-like 1 (3D3): m-IgG Fc BP-HRP 套装

sc-526629
200 µg Ab; 10 µg BP
RMB2662.00

Podocalyxin-like 1 (3D3): m-IgG1 BP-HRP 套装

sc-532002
200 µg Ab; 20 µg BP
RMB2662.00

Podocalyxin-like 1 抗体 (3D3) AC

sc-23904 AC
500 µg/ml, 25% agarose
RMB3129.00

Podocalyxin-like 1 抗体 (3D3) HRP

sc-23904 HRP
200 µg/ml
RMB2377.00

Podocalyxin-like 1 抗体 (3D3) FITC

sc-23904 FITC
200 µg/ml
RMB2482.00

Podocalyxin-like 1 抗体 (3D3) PE

sc-23904 PE
200 µg/ml
RMB2580.00

Podocalyxin-like 1 抗体 (3D3) Alexa Fluor® 488

sc-23904 AF488
200 µg/ml
RMB2685.00

Podocalyxin-like 1 抗体 (3D3) Alexa Fluor® 546

sc-23904 AF546
200 µg/ml
RMB2685.00

Podocalyxin-like 1 抗体 (3D3) Alexa Fluor® 594

sc-23904 AF594
200 µg/ml
RMB2685.00

Podocalyxin-like 1 抗体 (3D3) Alexa Fluor® 647

sc-23904 AF647
200 µg/ml
RMB2685.00

Podocalyxin-like 1 抗体 (3D3) Alexa Fluor® 680

sc-23904 AF680
200 µg/ml
RMB2685.00

Podocalyxin-like 1 抗体 (3D3) Alexa Fluor® 790

sc-23904 AF790
200 µg/ml
RMB2685.00

What would be your advise concerning the dilution to use the antibody in an ELISA ?

Asked by: Murielle
Thank you for your question. This antibody is not recommended for ELISA applications, so we do not have a recommended dilution for such assays with the product.
Answered by: Tech Support Europe
Date published: 2022-04-28

What is the recommended Antigen retrieval method for this antibody?

Asked by: DishaV
Thank you for your question. We recommend antigen retrieval with sodium citrate with heat at pH 6 when using paraffin embedded sections for IHC.
Answered by: Santa Cruz TS
Date published: 2021-07-05

What is the specific PODXL epitope recognized by the PODXL antibody (3D3; SC-23904?)

Asked by: Joe H
Thank you for your question. Podocalyxin-like 1 Antibody (3D3): sc-23904 has been raised against full-length protein of human origin. Since no epitope mapping has been performed for this antibody, the exact epitope is not known.
Answered by: Tech Support Europe
Date published: 2020-11-16

for the podexl 3d3 antibody how much protein should i load, 50ug or 100ug?

Asked by: Rich375
Thank you for your question. Depending on the type of sample you are using we recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. You can find additional information in our recommended western blotting protocol. You can find the protocol by following this link: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2019-06-26

I would like to perform some IHC staining on my mouse samples. Do you have any tips for doing this with a mouse monoclonal?

Asked by: Cweed
We recommend using a directly conjugated primary antibody. This product, sc-23904, is available directly conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 13
Date published: 2017-01-09
  • y_2025, m_9, d_7, h_4CST
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Rated 1 out of 5 by from IFUsed for IF on human T cells and saw only minimal/background staining.
Date published: 2017-11-20
Rated 5 out of 5 by from Just a great product !!Outstanding western data; strong, clear signal; also great IHC and IF data
Date published: 2017-01-26
Rated 5 out of 5 by from Reliable Antibody, Great for IHCThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-11
Rated 5 out of 5 by from Excellent immunoperoxidase membrane stainingExcellent immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-23
Rated 4 out of 5 by from Robust band seen in WB using Ecv304Robust band seen in WB using Ecv304, H9, Raji and MIA PaCa-2 whole cell lysates. -SCBT QC
Date published: 2015-03-07
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Podocalyxin-like 1 抗体 (3D3) is rated 4.0 out of 5 by 5.
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