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PMCA Antibody (H-8) - Western Blotting - Image 300452 — PMCA 抗体 (H-8): sc-271194. Ramos (A) 和 IMR-32 (B) 全细胞裂解液及小鼠脑组织提取物 (C) 中 PMCA 表达的 Western 印迹分析.

PMCA 抗体 (H-8): sc-271194. Ramos (A) 和 IMR-32 (B) 全细胞裂解液及小鼠脑组织提取物 (C) 中 PMCA 表达的 Western 印迹分析.

PMCA 抗体 (H-8): sc-271194

Name: PMCA抗体 (H-8)
Brand: SCBIO
SKU: sc-271194
Price: 2377.0 CNY
Availability: InStock

5.0(3)

寫評論提問

说明书

PMCA抗体(H-8)是小鼠单克隆IgG_2a κ, 在2篇文献中引用，规格为200 µg/ml
针对氨基酸481-780，在human起源的PMCA的细胞质域内进行映射
推荐用于 mouse, rat 和 human 来源的PMCA1, PMCA2, PMCA3 and PMCA4 WB, IP, IF, IHC(P) 和 ELISA检测
m-IgG Fc BP-HRP, m-IgG_2a BP-HRP 和 m-IgGκ BP-HRP 是 PMCA 抗体 (H-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PMCA 抗体 (H-8) 组合成套装提供。 (请参阅下面的订购信息)。

查看产品引用文献(2)

快捷链接

PMCA 抗体 (H-8) 参考文献:

质膜钙泵（PMCA）在钙调蛋白和磷脂酸激活后疏水结构域的不同暴露。 | Mangialavori, I., et al. 2011. J Biol Chem. 286: 18397-404. PMID: 21454645
SERCA和PMCA泵导致了人类CF上皮细胞中Ca2+稳态的失调。 | Philippe, R., et al. 2015. Biochim Biophys Acta. 1853: 892-903. PMID: 25661196
tau 对 PMCA 活性的抑制是衰老和阿尔茨海默氏症神经病理学的一种功能。 | Berrocal, M., et al. 2015. Biochim Biophys Acta. 1852: 1465-76. PMID: 25892185
磷脂和钙调素调节 tau 对 PMCA 活性的抑制。 | Berrocal, M., et al. 2017. Biochim Biophys Acta Mol Cell Res. 1864: 1028-1035. PMID: 27818274
亚甲基蓝能激活 PMCA 的活性，并与淀粉样β肽发生交叉作用，从而阻断 Aβ- 介导的 PMCA 抑制作用。 | Berrocal, M., et al. 2018. Neuropharmacology. 139: 163-172. PMID: 30003902
应用 PMCA 筛查用于生产生物疗法的人类细胞系中的朊病毒感染。 | Lyon, A., et al. 2019. Sci Rep. 9: 4847. PMID: 30890734
从致命家族性失眠症患者嗅粘膜中提取的 PMCA 生成的朊病毒会导致小鼠朊病毒病。 | Bistaffa, E., et al. 2021. Elife. 10: PMID: 33851575
Sorcin 激活大脑 PMCA 并阻断阿尔茨海默病分子标志物对泵活性的抑制作用。 | Berrocal, M., et al. 2021. Int J Mol Sci. 22: PMID: 34205207
CFTR 表达缺陷导致的 PMCA 活性调节受损会促进酒精性胰腺炎和肝炎的上皮细胞损伤。 | Madácsy, T., et al. 2022. Cell Mol Life Sci. 79: 265. PMID: 35484438
PLEKHA7-PDZD11复合物调节钙泵PMCA的定位和培养细胞中的钙处理。 | Sluysmans, S., et al. 2022. J Biol Chem. 298: 102138. PMID: 35714771

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
PMCA 抗体 (H-8)	sc-271194	200 µg/ml	RMB2377.00
PMCA (H-8): m-IgG Fc BP-HRP 套装	sc-537585	200 µg Ab; 10 µg BP	RMB2662.00
PMCA (H-8): m-IgGκ BP-HRP 套装	sc-534891	200 µg Ab; 40 µg BP	RMB2662.00
PMCA (H-8): m-IgG_2a BP-HRP 套装	sc-548058	200 µg Ab; 10 µg BP	RMB2662.00

Can PMCA (H-8): sc-271194 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero

Thank you for your question. Yes, PMCA (H-8): sc-271194 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-02-27

Rated 5 out of 5 by JT from Band detected at expected mw in HeLa wholeBand detected at expected mw in HeLa whole cell lysate. -SCBT QC

Date published: 2015-06-17

Rated 5 out of 5 by JM from Exceptional immunoperoxidase membrane andExceptional immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC

Date published: 2015-04-26

Rated 5 out of 5 by HF from GreatGreat, strong band in WB in Ramos and IMR-32 whole cell lysates and mouse brain tissue extract. -SCBT QC

Date published: 2015-03-28

PMCA 抗体 (H-8) is rated 5.0 out of 5 by 3.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	ATP2B4	Entrez Gene ID: 493	1q32.1	NM_001001396, NM_001684	UniProt ID: P23634	108732
Human	ATP2B1	Entrez Gene ID: 490	12q21.33	XM_011538407, XM_017019357, XM_017019358, XM_017019359, XM_017019360, NM_001001323, NM_001682	UniProt ID: P20020	108731
Human	ATP2B3	Entrez Gene ID: 492	Xq28	XM_005274689, XM_005274690, XM_005274691, XM_005274692, XM_011531174, XM_011531177, XM_017029553, NM_001001344, NM_021949	UniProt ID: Q16720	300014
Human	ATP2B2	Entrez Gene ID: 491	3p25.3	XM_005265179, XM_006713175, XM_011533752, XM_017006481, XM_017006482, XM_017006483, XM_017006484, XM_017006485, XM_017006486, XM_017006487, XM_017006488, XM_017006489, XM_017006490, XM_017006491, XM_017006492, NM_001001331, NM_001330611, NM_001683	UniProt ID: Q01814	601386
Mouse	Atp2b4	Entrez Gene ID: 381290	1 E4	XM_006529723, XM_006529724, XM_006529725, XM_006529726, XM_006529727, XM_006529728, XM_011248038, NM_001167949, NM_213616	UniProt ID: Q6Q477	N/A
Mouse	Atp2b1	Entrez Gene ID: 67972	10 C3	XM_006514015, XM_011243548, XM_011243549, XM_017314071, XM_017314072, NM_026482	UniProt ID: G5E829	N/A
Mouse	Atp2b2	Entrez Gene ID: 11941	6 E3	XM_011241161, XM_011241162, XM_011241163, XM_011241164, XM_011241165, XM_011241166, XM_011241167, XM_011241168, XM_011241169, XM_011241170, XM_017321353, XM_017321354, NM_001036684, NM_001347365, NM_009723	UniProt ID: Q9R0K7	N/A
Mouse	Atp2b3	Entrez Gene ID: 320707	X A7.3	XM_006528081, XM_006528084, XM_006528086, XM_011247609, XM_011247610, XM_011247611, XM_011247612, XM_011247613, XM_011247614, NM_001310537, NM_177236	UniProt ID: XP_006528144	N/A

PMCA 抗体 (H-8): sc-271194

PMCA 抗体 (H-8) 参考文献:

優選的 PMCA 抗體是 PMCA 抗體 (F-3): sc-271917.

PMCA 抗体 (H-8)

PMCA (H-8): m-IgG Fc BP-HRP 套装

PMCA (H-8): m-IgGκ BP-HRP 套装

PMCA (H-8): m-IgG2a BP-HRP 套装

Can PMCA (H-8): sc-271194 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

PMCA (H-8): m-IgG_2a BP-HRP 套装