Date published: 2025-9-7

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Plk 抗体 (F-8): sc-17783

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说明书
  • Plk 抗体 F-8 是小鼠单克隆 IgG2a κ,Plk抗体, 在247篇文献中引用,规格为200 µg/ml
  • 与氨基酸261-412结合,映射到human起源的Plk的内部区域
  • Plk 抗体 (F-8) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Plk
  • 抗Plk抗体(F-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Plk (F-8): sc-17783免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是Plk Antibody (F-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Plk Antibody (F-8) 打包提供(请参阅下面的订购信息)。

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関連項目

Plk抗体(F-8)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源Plk。Plk抗体(F-8)有非结合型和多种结合型,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合物。Plk(或称Polo样激酶1)是一种重要的丝氨酸/苏氨酸激酶,在细胞周期调节中发挥着重要作用,尤其是在有丝分裂期间。Plk对于有丝分裂纺锤体的正常运转至关重要,而纺锤体负责细胞分裂期间染色体的精确分离。Plk主要位于细胞质中,在细胞周期的S期和G2期积累,在细胞分裂开始时达到峰值,并在细胞分裂后迅速减少。这种动态表达模式凸显了Plk在细胞增殖中的重要性,并表明Plk可以作为有丝分裂活跃组织(包括结肠和胎盘以及各种肿瘤)的宝贵标记物。Plk抗体(F-8)能够帮助研究人员深入了解细胞分裂的机制以及Plk在癌症生物学中的潜在意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Plk 抗体 (F-8) 参考文献:

  1. 临床前和早期临床肿瘤学中Polo样激酶(PLK)抑制剂的研究。  |  Schöffski, P. 2009. Oncologist. 14: 559-70. PMID: 19474163
  2. 对真核生物中Plk介导的磷酸化调节的系统分析。  |  Liu, Z., et al. 2013. Brief Bioinform. 14: 344-60. PMID: 22851512
  3. PLK-1靶向抑制剂及其抗肿瘤作用。  |  Kumar, S. and Kim, J. 2015. Biomed Res Int. 2015: 705745. PMID: 26557691
  4. PLK-1及其底物的调控功能区域超出有丝分裂。  |  Kumar, S., et al. 2017. Oncotarget. 8: 37942-37962. PMID: 28415805
  5. polo是果蝇的一种有丝分裂突变体,显示出异常的纺锤极。  |  Sunkel, CE. and Glover, DM. 1988. J Cell Sci. 89 (Pt 1): 25-38. PMID: 3417791
  6. PLK抑制剂在儿童脑肿瘤治疗中崭露头角。  |  Maegawa, S. and Gopalakrishnan, V. 2022. Neuro Oncol. 24: 427-428. PMID: 35015893
  7. 编码一种保守的有丝分裂蛋白激酶的小鼠 mRNA 的细胞周期和末期分化缔合作用调控。  |  Lake, RJ. and Jelinek, WR. 1993. Mol Cell Biol. 13: 7793-801. PMID: 7902533
  8. 人类 Plk1 的细胞周期分析和染色体定位,Plk1 是果蝇有丝分裂激酶 polo 和酿酒酵母 Cdc5 的推定同源物。  |  Golsteyn, RM., et al. 1994. J Cell Sci. 107 (Pt 6): 1509-17. PMID: 7962193
  9. 果蝇 polo 丝氨酸-苏氨酸激酶的人类和鼠类同源物的克隆与表征。  |  Hamanaka, R., et al. 1994. Cell Growth Differ. 5: 249-57. PMID: 8018557
  10. PLK 是一种在增殖细胞和肿瘤中表达的人类丝氨酸/苏氨酸激酶。  |  Holtrich, U., et al. 1994. Proc Natl Acad Sci U S A. 91: 1736-40. PMID: 8127874
  11. 酿酒酵母 G1 细胞周期突变基因 dbf4 的一个多拷贝抑制基因编码一种蛋白激酶,被鉴定为 CDC5。  |  Kitada, K., et al. 1993. Mol Cell Biol. 13: 4445-57. PMID: 8321244
  12. 哺乳动物细胞的恶性转化是由组成型磷酸激酶的表达引发剂。  |  Smith, MR., et al. 1997. Biochem Biophys Res Commun. 234: 397-405. PMID: 9177283

订购信息

产品名称产品编号规格价格数量收藏夹

Plk 抗体 (F-8)

sc-17783
200 µg/ml
RMB2377.00

Plk (F-8): m-IgG Fc BP-HRP 套装

sc-528310
200 µg Ab; 10 µg BP
RMB2662.00

Plk (F-8): m-IgGκ BP-HRP 套装

sc-520654
200 µg Ab, 40 µg BP
RMB2662.00

Plk (F-8): m-IgG2a BP-HRP 套装

sc-547068
200 µg Ab; 10 µg BP
RMB2662.00

Plk 抗体 (F-8) AC

sc-17783 AC
500 µg/ml, 25% agarose
RMB3129.00

Plk 抗体 (F-8) HRP

sc-17783 HRP
200 µg/ml
RMB2377.00

Plk 抗体 (F-8) FITC

sc-17783 FITC
200 µg/ml
RMB2482.00

Plk 抗体 (F-8) PE

sc-17783 PE
200 µg/ml
RMB2580.00

Plk 抗体 (F-8) Alexa Fluor® 488

sc-17783 AF488
200 µg/ml
RMB2685.00

Plk 抗体 (F-8) Alexa Fluor® 546

sc-17783 AF546
200 µg/ml
RMB2685.00

Plk 抗体 (F-8) Alexa Fluor® 594

sc-17783 AF594
200 µg/ml
RMB2685.00

Plk 抗体 (F-8) Alexa Fluor® 647

sc-17783 AF647
200 µg/ml
RMB2685.00

Plk 抗体 (F-8) Alexa Fluor® 680

sc-17783 AF680
200 µg/ml
RMB2685.00

Plk 抗体 (F-8) Alexa Fluor® 790

sc-17783 AF790
200 µg/ml
RMB2685.00

Can I use this mouse monoclonal antibody for IHC staining of mouse tissue?

Asked by: Anna99
Yes, this antibody is available conjugated to HRP (sc-17783 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; and to either phycoerythrin (sc-17783 PE) or fluoroscein (sc-17783 FITC), Alexa Fluor® 488 (sc-17783 AF488) or Alexa Fluor® 647 (sc-17783 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2017-01-05

Is sc-17783 suitable for use on formalin-fixed paraffin-embedded tissues?

Asked by: TinTin
Thank you for your question. We have not tested the Plk (F-8) antibody for IHC(P) and so this is not a recommended application at this time. The antibody is recommended for, and has been published for IF.
Answered by: Technical Support
Date published: 2016-12-30
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Rated 4 out of 5 by from WB OKWe detected the expression of PLK in H1299 cells. 1:500
Date published: 2025-02-15
Rated 5 out of 5 by from Works great for western blot and IF1) Working dilution 1:6000 - 1:8000 on western blot depending on the amount of protein loaded. Clean bands that matched with expected size on the blot that was previously probed with phospho-PLK1 antibody. 2) Works well on IF at dilution 1:250. Note the clear PLK1 staining on the mitotic cells (metaphase vs anaphase)
Date published: 2021-11-04
Rated 5 out of 5 by from very good antibodyWe tested the antibody on western blots, it works very well.
Date published: 2021-05-06
Rated 4 out of 5 by from Gute bis sehr gute ErgebnisseSeit Jahren verwenden wir diesen Plk1-Antikörper, der in fast allen Zelllinien sehr gute Ergebnisse zeigt. Ausnahmen bilden lediglich einige Primärzellen, hier setzen wir ein Wettbewerberprodukt ein. Das ist sehr viel teurer, aber in diesen Spezialfällen geeigneter. Bei 95% unserer Blots sind wir jedoch sehr zufrieden mit dem Santa Cruz Plk1-Antikörper.
Date published: 2020-02-21
Rated 5 out of 5 by from Great antibodyThe PLK Antibody (F-8) works well in MCF7 breast cancer cells (1:500 dilution).
Date published: 2019-09-27
Rated 4 out of 5 by from Figure 5-DProtein analysis by Western blot analysis of PLK1 in three mutant lung adenocarcinoma cells (H1792.604, H2009, H358)
Date published: 2019-02-22
Rated 4 out of 5 by from Good staining in IFgood staining in immunofluorence on PFA fixed cells at 1:1000 dilution.
Date published: 2018-12-12
Rated 5 out of 5 by from Good antibody!1:200 diluted for wb of rat protein,the band is strong and special
Date published: 2018-04-11
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Plk 抗体 (F-8) is rated 4.7 out of 5 by 36.
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