Date published: 2025-9-8

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PLC β4 抗体 (D-7): sc-166133

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说明书
  • PLC β4抗体(D-7)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
  • 免疫human物种的PLC β4的氨基酸876-1115
  • 推荐用于 mouse, rat 和 human 来源的PLC β4 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是PLC β4 Antibody (D-7) for WB applications. 的首选辅助检测试剂,这些试剂现与PLC β4 Antibody (D-7) 打包提供(请参阅下面的订购信息)。

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    関連項目

    磷脂酰肌醇特异性磷脂酶C(PLC)对磷脂酰肌醇4,5二磷酸作用产生两种第二信使肌醇1,4,5-三磷酸和二酰基甘油,由此在启动受体介导的信号转导中起着关键作用。据报道总共有8种哺乳动物PLC同工酶(PLCβ1,PLCβ2,PLCβ3,PLCβ4,PLCγ1,PLCγ2,PLCδ1和PLCδ2)。γ型酶的独特之处在于它们含有SH2和SH3结构域。此外,两种γ型酶倾向于被多种能与其SH2结构域结合的蛋白酪氨酸激酶激活,并通过磷酸化诱导其激活,而β和δ型同工酶无法做到。相比之下,异三聚体G蛋白Gq的α亚基和某些G蛋白βγ亚基可以介导PLCβ1、PLC β2和PLC β3的激活作用。 PLCδ1和PLCδ2的调控机制尚未解决。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    PLC β4 抗体 (D-7) 参考文献:

    1. 使用简单的 RT-PCR 和溴化乙锭染色法对大鼠大脑中的相对 mRNA 表达进行定量。  |  Horikoshi, T. and Sakakibara, M. 2000. J Neurosci Methods. 99: 45-51. PMID: 10936641
    2. GLC1K 幼年原发性开角型青光眼基因座的精细图谱绘制及候选基因的排除。  |  Sud, A., et al. 2008. Mol Vis. 14: 1319-26. PMID: 18648523
    3. mGluR5+/+小鼠癫痫状态皮洛卡品模型中海马CA1锥体神经元的mGluR5-PLCbeta4-PKCbeta2/PKCgamma通路。  |  Liu, JX., et al. 2008. Epilepsy Res. 82: 111-23. PMID: 18774262
    4. 人类牙周韧带和骨髓间充质干细胞中长非编码 RNA 和 mRNA 表达谱的比较。  |  Dong, R., et al. 2014. Biomed Res Int. 2014: 317853. PMID: 24790996
    5. 丘脑 mGluR1-PLCβ4 通路对睡眠结构至关重要。  |  Hong, J., et al. 2016. Mol Brain. 9: 100. PMID: 27998287
    6. 罗格列酮通过 Gαq/Plcβ4/TRPC 信号通路抑制血管紧张素 II 诱导的肾小球间质细胞增殖  |  Wei, L., et al. 2017. Cell Physiol Biochem. 44: 2228-2242. PMID: 29258063
    7. 中国葡萄膜黑色素瘤患者的 GNAQ、GNA11、SF3B1、EIF1AX、PLCB4 和 CYSLTR 基因突变  |  Hou, C., et al. 2020. Ophthalmic Res. 63: 358-368. PMID: 31614358
    8. 葡萄膜黑色素瘤中 G 蛋白相关通路的继发性体细胞突变和突变特征  |  Piaggio, F., et al. 2019. Cancers (Basel). 11: PMID: 31671564
    9. 三七皂苷体外抑制猪流行性腹泻病毒:评估抗病毒潜力。  |  Hu, Y., et al. 2024. Arch Virol. 169: 89. PMID: 38565720
    10. 在 p53 诱导的细胞凋亡中分离出 10 个差异表达的 cDNAs:激活脊椎动物同源的果蝇缺席七基因。  |  Amson, RB., et al. 1996. Proc Natl Acad Sci U S A. 93: 3953-7. PMID: 8632996

    订购信息

    产品名称产品编号规格价格数量收藏夹

    PLC β4 抗体 (D-7)

    sc-166133
    200 µg/ml
    RMB2377.00

    PLC β4 (D-7): m-IgG Fc BP-HRP 套装

    sc-540022
    200 µg Ab; 10 µg BP
    RMB2662.00

    PLC β4 (D-7): m-IgG1 BP-HRP 套装

    sc-541850
    200 µg Ab; 20 µg BP
    RMB2662.00

    For Western Blot, is it recommended to use denatured or non-denatured conditions with PLC β4 (D-7): sc-166133 monoclonal antibody?

    Asked by: Trav11
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 4 out of 5 by from Light band seen in WB using ESLight band seen in WB using ES-2 whole cell lysate. -SCBT QC
    Date published: 2015-03-29
    Rated 4 out of 5 by from Strong band detected in WB using A549 wholeStrong band detected in WB using A549 whole cell lysate. -SCBT QC
    Date published: 2015-01-12
    Rated 4 out of 5 by from Robust band seen in WB using mouse cerebellumRobust band seen in WB using mouse cerebellum and rat cerebellum tissue extracts. -SCBT QC
    Date published: 2013-07-15
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    PLC β4 抗体 (D-7) is rated 4.0 out of 5 by 3.
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