Date published: 2025-9-7

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PLAP 抗体 (8B6): sc-47691

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说明书
  • PLAP 抗体 8B6 是小鼠单克隆 IgG2a κ,PLAP抗体, 在18篇文献中引用,规格为200 µg/ml
  • 针对表达PLAP的整个细胞(Hep-2)
  • PLAP 抗体 (8B6) 推荐用于WB, IP, IF 和 IHC(P),检测human 来源的 PLAP
  • 抗PLAP抗体(8B6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PLAP (8B6): sc-47691免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 PLAP 抗体 (8B6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PLAP 抗体 (8B6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

PLAP抗体(8B6)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHCP)检测人类来源的PLAP蛋白。PLAP (8B6) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。胎盘碱性磷酸酶(PLAP)蛋白,也称为ALPP,是一种由530个氨基酸组成的糖蛋白,在细胞过程中起着至关重要的作用,它催化磷酸单酯水解为无机磷酸盐和酒精,这对各种代谢途径至关重要。PLAP主要在胎盘中表达,也存在于孕妇的血清中,在某些癌症(如卵巢和睾丸肿瘤)中观察到异位表达。这些组织中的PLAP表达表明其参与引导迁移细胞和促进特定分子(包括脂肪酸和免疫球蛋白)穿过质膜的转运,这对于维持细胞稳态和支持肿瘤进展至关重要。值得注意的是,PLAP与生殖细胞碱性磷酸酶(GCAP)和肠道碱性磷酸酶(IAP)都是组织特异性碱性磷酸酶,它们具有90-98%的同源性,聚集在2q染色体上,这突出了它们在人类生理学中的进化关系和功能意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PLAP 抗体 (8B6) 参考文献:

  1. 分辨率为 1.8 A 的人类胎盘碱性磷酸酶晶体结构。底物特异性的含义。  |  Le Du, MH., et al. 2001. J Biol Chem. 276: 9158-65. PMID: 11124260
  2. 子宫内膜异位症妇女腹腔液中的胎盘型碱性磷酸(酯)酶。  |  Kang, JO., et al. 1990. Clin Chim Acta. 186: 285-94. PMID: 2311256
  3. 人胃癌细胞产生的胎盘碱性磷酸(酯)酶同工酶  |  Aizawa, K., et al. 1989. Acta Pathol Jpn. 39: 630-7. PMID: 2686348
  4. 血清胎盘样碱性磷酸酶(PLAP):用于监测卵巢癌的新型酶联免疫测定。  |  Fisken, J., et al. 1989. J Clin Pathol. 42: 40-5. PMID: 2921344
  5. 通过 H17E2 单克隆抗体测定评估睾丸生殖细胞肿瘤中胎盘碱性磷酸酶样活性的血清标记物潜力。  |  Tucker, DF., et al. 1985. Br J Cancer. 51: 631-9. PMID: 2986664
  6. 碱性磷酸(酯)酶及其同工酶的诊断方面。  |  Moss, DW. 1987. Clin Biochem. 20: 225-30. PMID: 3319285
  7. 人类胎盘和肠道碱性磷酸(酯)酶基因位于2q34-q37。  |  Griffin, CA., et al. 1987. Am J Hum Genet. 41: 1025-34. PMID: 3687940
  8. 监测睾丸肿瘤的血清胎盘碱性磷酸酶单克隆抗体测定。  |  Epenetos, AA., et al. 1985. Br J Cancer. 51: 641-4. PMID: 3994908
  9. 针对胎盘碱性磷酸酶和其他人类滋养层相关决定因子的单克隆抗体的制备和表征。  |  Travers, P. and Bodmer, W. 1984. Int J Cancer. 33: 633-41. PMID: 6202646
  10. 使用两种不同的胎盘碱性磷酸酶单克隆抗体对睾丸生殖细胞瘤进行免疫组织学研究。  |  Epenetos, AA., et al. 1984. Br J Cancer. 49: 11-5. PMID: 6362705
  11. 人类碱性磷酸(酯)酶生物学,特别是癌症。  |  Millán, JL. and Fishman, WH. 1995. Crit Rev Clin Lab Sci. 32: 1-39. PMID: 7748466
  12. 人类恶性肿瘤中胎盘碱性磷酸酶的表达概况:肿瘤细胞活化对碱性磷酸酶表达的影响。  |  Dabare, AA., et al. 1999. Urol Int. 63: 168-74. PMID: 10738188

订购信息

产品名称产品编号规格价格数量收藏夹

PLAP 抗体 (8B6)

sc-47691
200 µg/ml
RMB2377.00

PLAP (8B6): m-IgG Fc BP-HRP 套装

sc-528505
200 µg Ab; 10 µg BP
RMB2662.00

PLAP (8B6): m-IgGκ BP-HRP 套装

sc-520899
200 µg Ab, 40 µg BP
RMB2662.00

PLAP (8B6): m-IgG2a BP-HRP 套装

sc-547111
200 µg Ab; 10 µg BP
RMB2662.00

PLAP 抗体 (8B6) AC

sc-47691 AC
500 µg/ml, 25% agarose
RMB3129.00

PLAP 抗体 (8B6) HRP

sc-47691 HRP
200 µg/ml
RMB2377.00

PLAP 抗体 (8B6) FITC

sc-47691 FITC
200 µg/ml
RMB2482.00

PLAP 抗体 (8B6) PE

sc-47691 PE
200 µg/ml
RMB2580.00

PLAP 抗体 (8B6) Alexa Fluor® 488

sc-47691 AF488
200 µg/ml
RMB2685.00

PLAP 抗体 (8B6) Alexa Fluor® 546

sc-47691 AF546
200 µg/ml
RMB2685.00

PLAP 抗体 (8B6) Alexa Fluor® 594

sc-47691 AF594
200 µg/ml
RMB2685.00

PLAP 抗体 (8B6) Alexa Fluor® 647

sc-47691 AF647
200 µg/ml
RMB2685.00

PLAP 抗体 (8B6) Alexa Fluor® 680

sc-47691 AF680
200 µg/ml
RMB2685.00

PLAP 抗体 (8B6) Alexa Fluor® 790

sc-47691 AF790
200 µg/ml
RMB2685.00

SC has two antibodies for human PLAP (G-9 and 8B6). 8B6, is more widely cited, but why is the single band seen by WB not 70kDa (dimer?) while many bands for G-9? Also do you have any blots showing KD with your siRNA to PLAP (sc-38919) for either antibody?

Asked by: SCONE
Thank you for your question. There are three forms of the PLAP protein that can be detected with these antibodies (~67 kD=monomeric,~130 kD=dimeric & ~180 kD=trimeric). We generally see the monomeric or dimeric in our sampels. Since PLAP expression differs in tumor cell lines, what form will be seen varies depending on the samples. Unfortunately we do not have siRNA data for this target with these antibodies, so we are unable to provide that.
Answered by: Tech Service
Date published: 2019-09-26

人胎盘组织裂解液WB的分子量是120KD吗?

Asked by: Hechengjin
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21

Can PLAP (8B6): sc-47691 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PLAP (8B6): sc-47691 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background.
Date published: 2017-02-03
Rated 5 out of 5 by from Publishable IF data with human placentalPublishable IF data with human placental trophoblast cells - SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from WB and IF publishable data with human transfectedWB and IF publishable data with human transfected MDCK - SCBT Publication Review
Date published: 2014-03-11
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PLAP 抗体 (8B6) is rated 5.0 out of 5 by 3.
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