Date published: 2025-9-6
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PKC zeta Antibody (H-1) - Western Blotting - Image 390790
PKC ζ 抗体 (H-1) Alexa Fluor® 546: sc-17781 AF546. 3611-RF (A), PC-3 (B) 和 SK-BR-3 (C) 全细胞裂解液中 PKC ζ 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
PKC ζ 抗体 (H-1) Alexa Fluor® 546: sc-17781 AF546. 3611-RF (A), PC-3 (B) 和 SK-BR-3 (C) 全细胞裂解液中 PKC ζ 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

PKC zeta 抗体 (H-1): sc-17781

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说明书
  • PKC zeta 抗体 H-1 是小鼠单克隆 IgG2a κ，PKC zeta抗体, 在146篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于rat物种的PKC Ω的C-terminus的氨基酸573-592之间
  • PKC zeta 抗体 (H-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PKC ζ; 可能与 PKC ι and PKC λ产生交联反应
  • 抗PKC zeta抗体（H-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PKC zeta (H-1): sc-17781的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 PKC zeta 抗体 (H-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PKC zeta 抗体 (H-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外PKC抗体，包括PKC α, PKC β, PKC δ, PKC ε, PKC η, PKC γ, PKC ι, PKC λ, PKC μ和PKC ν

PKC ζ 抗体（H-1）是一种小鼠单克隆IgG2a抗体，靶向大鼠源性蛋白激酶C zeta（PKC ζ）C末端573-592位氨基酸之间的表位，也称为PRKCZ或nPKC-ζ。抗PKC ζ抗体（H-1）可与小鼠、大鼠和人类反应，适用于包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）在内的多种应用。PKCζ是蛋白激酶C家族中一个非典型的成员，在细胞生长、分化、存活、基因表达和膜功能等各种细胞过程中发挥着关键作用。与传统的PKC不同，PKCζ的激活与钙和二酰基甘油（DAG）无关，在信号传导通路中具有独特功能。PKCζ参与NF-κB激活、胰岛素信号传导、细胞极性和PI3K/Akt通路的调节，因此对于了解糖尿病、癌症和免疫失调等疾病具有重要意义。PKC ζ参与细胞存活和增殖，这凸显了PKC ζ作为潜在治疗靶点的重要性。此外，独特的激活机制使PKC ζ成为信号转导研究中一个引人入胜的主题，有可能揭示细胞通讯途径的新见解。抗PKC ζ抗体（H-1）使研究人员能够有效地研究PKC ζ在各种生物学环境中的表达和功能，有助于开发新的治疗方法。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PKC zeta 抗体 (H-1) 参考文献:

  1. 蛋白激酶 C 家族的新成员 nPKC theta 主要在骨骼肌中表达。  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
  2. 信号转导蛋白激酶家族（蛋白激酶 C 家族）的结构和功能多样性；两类不同的 PKC，即传统的 cPKC 和新型的 nPKC。  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
  3. 蛋白激酶 C-delta 的表达和特征。  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
  4. 马血小板中的PKC同工酶及其受刺激诱导的激活。  |  Aktan, I., et al. 2011. Vet Immunol Immunopathol. 141: 276-82. PMID: 21513989
  5. PKC-ζ通过抑制Wnt/β-Catenin信号传导加重阿霉素诱导的心脏毒性。  |  Cao, YJ., et al. 2022. Front Pharmacol. 13: 798436. PMID: 35237161
  6. PKC-ζ介导的细胞外囊泡相关TGF-β1减少克服了乳腺癌的放射治疗耐药性。  |  Zhang, F., et al. 2023. Breast Cancer Res. 25: 38. PMID: 37029374
  7. 膜磷脂对多功能蛋白激酶的钙依赖性激活。  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
  8. 肌醇磷脂的周转和信号转导  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
  9. 蛋白激酶 C 在细胞表面信号转导和肿瘤促进中的作用。  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
  10. 蛋白激酶 C 可能是促进肿瘤生长的植物醇酯的受体蛋白。  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
  11. 促进肿瘤生长的植物醇酯直接激活钙激活的磷脂依赖性蛋白激酶。  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
  12. 兔PKC zeta的肾内分布。  |  Hao, CM., et al. 1997. Kidney Int. 51: 1831-7. PMID: 9186872

订购信息

产品名称产品编号规格价格数量收藏夹

PKC zeta 抗体 (H-1)

sc-17781
200 µg/ml
RMB2377.00

PKC zeta (H-1): m-IgG Fc BP-HRP 套装

sc-528309
200 µg Ab; 10 µg BP
RMB2662.00

PKC zeta (H-1): m-IgGκ BP-HRP 套装

sc-520653
200 µg Ab, 40 µg BP
RMB2662.00

PKC zeta (H-1): m-IgG2a BP-HRP 套装

sc-547067
200 µg Ab; 10 µg BP
RMB2662.00

PKC zeta 抗体 (H-1) AC

sc-17781 AC
500 µg/ml, 25% agarose
RMB3129.00

PKC zeta 抗体 (H-1) HRP

sc-17781 HRP
200 µg/ml
RMB2377.00

PKC zeta 抗体 (H-1) FITC

sc-17781 FITC
200 µg/ml
RMB2482.00

PKC zeta 抗体 (H-1) PE

sc-17781 PE
200 µg/ml
RMB2580.00

PKC zeta 抗体 (H-1) Alexa Fluor® 488

sc-17781 AF488
200 µg/ml
RMB2685.00

PKC zeta 抗体 (H-1) Alexa Fluor® 546

sc-17781 AF546
200 µg/ml
RMB2685.00

PKC zeta 抗体 (H-1) Alexa Fluor® 594

sc-17781 AF594
200 µg/ml
RMB2685.00

PKC zeta 抗体 (H-1) Alexa Fluor® 647

sc-17781 AF647
200 µg/ml
RMB2685.00

PKC zeta 抗体 (H-1) Alexa Fluor® 680

sc-17781 AF680
200 µg/ml
RMB2685.00

PKC zeta 抗体 (H-1) Alexa Fluor® 790

sc-17781 AF790
200 µg/ml
RMB2685.00

PKC zeta (H-1) 中和勝肽

sc-17781 P
100 µg/0.5 ml
RMB511.00

The previous antibody (SC216) which this one has replaced worked well for IF in Drosophila tissues - is this the case of this antibody?

Asked by: Holpol
Thank you for your question. sc-17781 has not been validated to work in Drosophila. There is a 75% homology against Drosophila. We only honor the warranty if the homology is 95% or higher .
Answered by: BlakeJ
Date published: 2023-03-22

does this antibody detect the phosphorylated form of apkc zeta?

Asked by: marti
Thank you for your question. PKC ζ Antibody (H-1): sc-17781 detects both phosphorylated and unphosphorylated PKC ζ.
Answered by: Tech Support Europe
Date published: 2019-09-02

does this antibody can analyse the expression of PKMzeta protein in brain tissue?

Asked by: mehar
Thank you for your question. Yes, we have used this mouse monoclonal antibody, PKC ζ (H-1): sc-17781 for immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue. This data is available on our website. This antibody is also very popular, and has been featured in over 30 publications. You can find a complete list of product citations for this antibody on our website, and you can search to see how others have used this antibody for IHC in brain tissue.
Answered by: Technical Support
Date published: 2022-10-31

What is the best secondary to use with PKC ζ Antibody (H-1): sc-17781 in Western Blot?

Asked by: jenni
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-09
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Rated 5 out of 5 by from Super produit a naturelJe souhaite acheter se produit apres essai pour m assurer qu il n y a pas d effet secondaire
Date published: 2024-05-31
Rated 4 out of 5 by from WBI used this antibody for WB. I could detect clear bands.
Date published: 2021-01-05
Rated 5 out of 5 by from 抗体很好用，效果比预期的要好之前从公司要了试用的一小管抗体，回来后自己做免疫荧光实验，发现效果很好，准本直接从公司购买正式投入实验使用。
Date published: 2018-02-28
Rated 4 out of 5 by from Very good in IFGood apical signal in IF on mIMCD3 grown as spheroids in matrigel. Concentration 1/400.
Date published: 2017-11-07
Rated 5 out of 5 by from Excellent AntibodyI used this to determine the PKC zeta levels expressed in melanoma cell lines MeWo and SK-MEL-2. WB worked really well with 1:2000 dilution, (4% BSA, 0.05% TBST).
Date published: 2017-10-24
Rated 5 out of 5 by from Great productWe used this antibody for our western blot. give us good results.
Date published: 2017-09-27
Rated 5 out of 5 by from Expressions were much higherIn the experiment, PKC ζ Antibody has good quality in Western blot, strong bands at 1:600 dilution, expressions were much higher.
Date published: 2017-09-04
Rated 5 out of 5 by from good stainingThis antibody works in staining of human kidney at 1:250 dilution. I am giving full stars as the staining is very stong and specific and antibody concentration needed very low.
Date published: 2017-08-19
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PKC zeta 抗体 (H-1) is rated 4.8 out of 5 by 34.
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