Date published: 2025-9-6
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PKC Antibody (MC5) - Western Blotting - Image 380254
PKC 抗体 (MC5): sc-80. Jurkat (A), SUP-T1 (B), Caki-1 (C) 和 C6 (D) 全细胞裂解液中 PKC 表达的 Western 印迹分析.
PKC 抗体 (MC5): sc-80. Jurkat (A), SUP-T1 (B), Caki-1 (C) 和 C6 (D) 全细胞裂解液中 PKC 表达的 Western 印迹分析.

PKC 抗体 (MC5): sc-80

4.9(9)
寫評論提問

说明书
  • PKC抗体(MC5)是小鼠单克隆IgG2a κ, 在103篇文献中引用，规格为200 µg/ml
  • bovine起源的PKC铰链区域（残基292-317）内的表位图谱
  • 抗-PKC 抗体 (MC5) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 PKC α, PKC β and PKC γ; 也和以下物种反应，包括： bovine
  • 抗PKC抗体（MC5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PKC (MC5): sc-80的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 PKC 抗体 (MC5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PKC 抗体 (MC5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加PKC抗体，包括PKC α, PKC β, PKC δ, PKC ε, PKC η, PKC γ, PKC ι, PKC λ, PKC μ和PKC ν

PKC抗体（MC5）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠和人类样本中的PKC。PKC (MC5) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。蛋白激酶C（PKC）家族对于调节细胞生长、分化、基因表达、激素分泌和膜动力学等广泛细胞功能至关重要。PKC蛋白主要位于细胞质中，激活后转移到细胞膜，这对于其在信号转导通路中的作用至关重要。这种转移对于下游信号级联的激活至关重要，从而影响各种生理过程。PKC同工型受二酰基甘油和肿瘤促进剂苯氧乙醇酯激活，可分为传统同工型和新型同工型，每种同工型具有不同的调节机制和组织特异性表达模式。了解PKC的定位和激活对于阐明其在健康和疾病中的作用至关重要，特别是在信号通路被破坏的癌症和其他病理中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

PKC 抗体 (MC5)

sc-80
200 µg/ml
RMB2377.00

PKC (MC5): m-IgG Fc BP-HRP 套装

sc-528095
200 µg Ab; 10 µg BP
RMB2662.00

PKC (MC5): m-IgGκ BP-HRP 套装

sc-520393
200 µg Ab, 40 µg BP
RMB2662.00

PKC (MC5): m-IgG2a BP-HRP 套装

sc-547023
200 µg Ab; 10 µg BP
RMB2662.00

PKC 抗体 (MC5) AC

sc-80 AC
500 µg/ml, 25% agarose
RMB3129.00

PKC 抗体 (MC5) HRP

sc-80 HRP
200 µg/ml
RMB2377.00

PKC 抗体 (MC5) FITC

sc-80 FITC
200 µg/ml
RMB2482.00

PKC 抗体 (MC5) PE

sc-80 PE
200 µg/ml
RMB2580.00

PKC 抗体 (MC5) Alexa Fluor® 488

sc-80 AF488
200 µg/ml
RMB2685.00

PKC 抗体 (MC5) Alexa Fluor® 546

sc-80 AF546
200 µg/ml
RMB2685.00

PKC 抗体 (MC5) Alexa Fluor® 594

sc-80 AF594
200 µg/ml
RMB2685.00

PKC 抗体 (MC5) Alexa Fluor® 647

sc-80 AF647
200 µg/ml
RMB2685.00

PKC 抗体 (MC5) Alexa Fluor® 680

sc-80 AF680
200 µg/ml
RMB2685.00

PKC 抗体 (MC5) Alexa Fluor® 790

sc-80 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2017-02-02
  • y_2025, m_9, d_6, h_5CST
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Rated 5 out of 5 by from Excellent detectionI took great signals from all the lysates from lung adenocarcinoma cell lines
Date published: 2021-03-17
Rated 4 out of 5 by from Está bienEste anticuerpo recién comprado está bien pero da un poco de señal-ruido de fondo en inmunohistoquímica.
Date published: 2017-05-11
Rated 5 out of 5 by from highly specificEyeballs were fixed in 4% Paraformaldehyde for 2h. Immunoreactivity in the mole-rat retina was observed exclusively in bipolar cells with this highly specific ab.
Date published: 2017-02-08
Rated 5 out of 5 by from Good WB data with rat skeletal muscleRecently，I used this monoclonal antibody 1：1000 with rat skeletal muscle，obtained the good bands which is my desired。
Date published: 2017-01-13
Rated 5 out of 5 by from Very specific antibody fo western blotWe use this antibody for western blot and we got a very specific clear band around the correct molecular weight
Date published: 2017-01-11
Rated 5 out of 5 by from Produced a clear band with human recombinant PKCProduced a clear band with human recombinant PKC . -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Works well for WB with NIH/3T3 and JurkatWorks well for WB with NIH/3T3 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by from Great staining with paraffinGreat staining with paraffin-embedded human gall bladder marrow. -SCBT QC
Date published: 2015-01-26
  • y_2025, m_9, d_6, h_5
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PKC 抗体 (MC5) is rated 4.9 out of 5 by 9.
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