Date published: 2025-9-6

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PKAα cat 抗体 (C-7): sc-48412

4.9(8)
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说明书
  • PKAα cat抗体(C-7)是小鼠单克隆IgG2a κ, 在5篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的PKAα的C-terminus的氨基酸322-351之间
  • 推荐用于 mouse, rat 和 human 来源的PKAα catalytic subunit WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应,包括: 和 equine, bovine and porcine; 可能与 cross-reactive with β and γ subunits产生交联反应
  • 看 PKAα cat (A-2): sc-28315,可查看有标记的 PKAα cat 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP 是 PKAα cat 抗体 (C-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与PKAα cat 抗体 (C-7) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

PKAα cat 抗体(C-7)是一种小鼠单克隆 IgG2a kappa 轻链抗体,可精确靶向蛋白激酶 A 催化亚基α(PKAα cat)。抗 PKAα cat 抗体(C-7)可识别映射到人 PKAα 催化亚基 C 端 322-351 氨基酸上的独特表位,确保在包括小鼠、大鼠、人、马、牛和猪在内的多个物种中进行选择性检测,还可能与 β 和 γ 催化亚基发生交叉反应。PKAα cat 是 cAMP 依赖性蛋白激酶 A(PKA)全酶的重要组成部分,在介导增殖、离子转运、代谢调节和基因转录等多种细胞反应中发挥着关键作用。从结构上看,PKAα 猫由一个对催化活性至关重要的保守激酶结构域和一个通过与 cAMP 结合调节功能的调节结构域组成。翻译后修饰,尤其是磷酸化,对于调节 PKAα cat 的活性、稳定性以及与其他蛋白质的相互作用至关重要,从而对所支配的信号通路进行微调。PKAα cat 单克隆抗体(C-7)能识别蛋白质的这些修饰形式,从而提高了研究 PKA 在各种生理和病理环境中动态调节的效用。无论是用于免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学还是酶联免疫吸附试验(ELISA),PKAα cat (C-7) 抗体都为研究人员提供了研究 PKA 激活复杂机制的可靠工具、 包括 cAMP 与调节亚基结合后催化亚基的释放,以及随后关键底物(如丝氨酸 133 上的 cAMP 反应元件结合蛋白 (CREB))的磷酸化。通过促进对 PKAα cat 结构和修饰的详细分析,抗 PKAα cat 抗体 (C-7) 有助于阐明信号转导途径及其对细胞功能的影响,因此是高级生物医学研究中不可或缺的试剂。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

PKAα cat 抗体 (C-7)

sc-48412
200 µg/ml
RMB2377.00

PKAα cat (C-7): m-IgGκ BP-HRP 套装

sc-520938
200 µg Ab, 40 µg BP
RMB2662.00

PKAα cat (C-7) 中和勝肽

sc-48412 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-48412 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good product!I tested this product for western blot. I used it at a dilution of 1: 500 over night at 4 ° C on cytoplasmic and nuclear lysates of Jurkat cells. I found an excellent functioning of the antibody that gives a very clear specific signal (40 kDa). To note the presence of a non-specific signal of about 35 kDa in nuclear lysates. I also tested this antibody for immunofluorescence by using it at a dilution of 1: 100 overnight at 4 ° C on Jurkat cells fixed in either paraformaldehyde or methanol.
Date published: 2021-10-12
Rated 5 out of 5 by from Good performanceStrong and clean signals in Western blot analysis were performed in non transfected 293T PKA alpha cats: (a) human sc-117752 PKA alpha cat transfected 293T:sc-115793 (b) and (c) A549 cell lysate.
Date published: 2017-02-09
Rated 5 out of 5 by from Thanks to my colleaguesOur colleagues, using a second messenger loop AMP, mediate various cellular responses to external signals, such as proliferation, ion transport, metabolic regulation, and gene transcription, by activating cAMP-dependent protein kinase A, with a very good overall effect
Date published: 2017-02-03
Rated 5 out of 5 by from Robust signal in WB in human transfectedRobust signal in WB in human transfected 293T and A549 whole cell lysates. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Published for WB in rat TMJ periarticularPublished for WB in rat TMJ periarticular tissue at 1:500 dilution. -SCBT Publication Review
Date published: 2015-02-11
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Good specificity and signal strength in WBGood specificity and signal strength in WB in human transfected 293T and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-02-02
Rated 5 out of 5 by from Good publishable data in WB in rat tissueGood publishable data in WB in rat tissue sections from rat frontal cortex. -SCBT Publication Review
Date published: 2015-01-02
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PKAα cat 抗体 (C-7) is rated 4.9 out of 5 by 8.
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