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PKAα cat 抗体 (B-5): sc-28316

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说明书
  • PKAα cat抗体(B-5)是小鼠单克隆IgG1 κ, 在2篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的PKAα的C-terminus的氨基酸322-351之间
  • 推荐用于 mouse, rat 和 human 来源的PKAα catalytic subunit WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应,包括: 和 equine, bovine and porcine; 可能与 β and γ subunits产生交联反应
  • 看 PKAα cat (A-2): sc-28315,可查看有标记的 PKAα cat 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 PKAα cat 抗体 (B-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PKAα cat 抗体 (B-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

PKAα cat抗体(B-5)是一种小鼠单克隆IgG1 kappa轻链抗体,旨在以极高的精确度靶向蛋白激酶A(PKA)α催化亚基(PRKACA)。抗PKAα猫抗体(B-5)识别人类PKAα催化亚基C末端氨基酸322-351的表位,确保在多种物种(包括小鼠、大鼠和人类)的蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF )、免疫组织化学和酶联免疫吸附测定(ELISA)应用中,在多种物种(包括小鼠、大鼠和人类)以及马、牛和猪等其他物种中均可实现可靠检测,但可能与PKA的β和γ亚基发生交叉反应。PKAα猫是cAMP依赖性蛋白激酶A(PKA)信号传导通路的关键组成部分,在调节细胞增殖、离子转运、代谢和基因转录等细胞过程方面发挥着关键作用。在静止状态下,PKAα cat主要位于细胞质中,在环磷酸腺苷(cAMP)激活后,它会转移到细胞核中,在那里对关键转录因子(如CREB(cAMP反应元件结合蛋白))的丝氨酸133进行磷酸化。这种磷酸化作用对于启动基因转录至关重要,它将细胞外信号与基因表达调控联系起来,并影响细胞功能和行为。PKAα cat在细胞内的精确定位和激活对于细胞对各种刺激做出适当反应至关重要,这使得PKAα cat成为信号转导通路及其在多种疾病中的影响研究的重要靶标。PKAα cat 单克隆抗体(B-5)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物,为适应不同的实验需求提供了多种选择,有助于对PKA介导的细胞过程及其在细胞内的空间动态机制进行深入研究。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

PKAα cat 抗体 (B-5)

sc-28316
200 µg/ml
RMB2377.00

PKAα cat (B-5): m-IgG Fc BP-HRP 套装

sc-536711
200 µg Ab; 10 µg BP
RMB2662.00

PKAα cat (B-5): m-IgGκ BP-HRP 套装

sc-533871
200 µg Ab; 40 µg BP
RMB2662.00

PKAα cat (B-5): m-IgG1 BP-HRP 套装

sc-544777
200 µg Ab; 20 µg BP
RMB2662.00

PKAα cat (B-5) 中和勝肽

sc-28316 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-28316 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good publishable data in WB in human normal (NGood publishable data in WB in human normal (N-HSFs) and DS human foetal skin fibroblast(DS-HSFs) cell lines. -SCBT Publication Review
Date published: 2015-03-05
Rated 5 out of 5 by from Strong band in WB in MCF7Strong band in WB in MCF7, PC-3, NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Good signal strength in WB in human transfectedGood signal strength in WB in human transfected 293T and A549 whole cell lysates. -SCBT QC
Date published: 2015-02-21
Rated 5 out of 5 by from Good specificity in WB in NIH/3T3 whole cellGood specificity in WB in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-02-11
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-01-26
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PKAα cat 抗体 (B-5) is rated 5.0 out of 5 by 5.
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