Date published: 2025-9-7

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PKAα/β/γ cat 抗体 (B-4): sc-365615

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说明书
  • PKA alpha / beta / gamma cat 抗体 B-4 是小鼠单克隆 IgG2b κ,PKAα/β/γ cat抗体, 在22篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列226-320位于human物种的PKAα cat的C-terminus附近
  • PKA alpha / beta / gamma cat 抗体 (B-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 PKAα cat, PKAβ cat and PKAγ cat
  • 抗PKA alpha / beta / gamma cat抗体(B-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PKAα/β/γ cat (B-4): sc-365615免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是PKAα/β/γ cat Antibody (B-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PKAα/β/γ cat Antibody (B-4) 打包提供(请参阅下面的订购信息)。

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関連項目

PKAα/β/γ cat抗体(B-4)是一种IgG2b κ小鼠单克隆PKA α/β/γ cat抗体(也称为PRKACA抗体、PRKACB抗体、PRKACG抗体或蛋白激酶cAMP激活催化亚基抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的PKA α/β/γ cat蛋白。PKAα/β/γ cat抗体(B-4)既可作为非偶联抗PKA α/β/γ cat抗体形式提供,也可作为多种偶联形式的抗PKA α/β/γ cat抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。第二信使环磷酸腺苷(cAMP)通过激活cAMP依赖性蛋白激酶(cAPK或PKA),介导细胞对外部信号的各种反应,如增殖、离子转运、代谢调节和基因转录。当cAMP与四聚体PKA全酶的两个调节亚基结合时,PKA被激活,从而释放出活性催化亚基。已鉴定出三种催化(C)亚基,分别命名为Cα、Cβ和Cγ,每种亚基都代表特定的基因产物。Cα和Cβ密切相关(93%的氨基酸序列相似性),而Cγ与Cα和Cβ的相似性分别为83%和79%。cAMP水平升高导致转录激活的原因是PKA易位至细胞核,在丝氨酸133处磷酸化转录因子cAMP反应元件结合蛋白(CREB),进而导致TFIIB与TATA盒结合蛋白TBP1结合,从而将磷酸化CREB与pol II转录起始复合物连接起来。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

PKAα/β/γ cat 抗体 (B-4)

sc-365615
200 µg/ml
RMB2377.00

PKAα/β/γ cat (B-4): m-IgG Fc BP-HRP 套装

sc-529409
200 µg Ab; 10 µg BP
RMB2662.00

PKAα/β/γ cat (B-4): m-IgGκ BP-HRP 套装

sc-522293
200 µg Ab, 40 µg BP
RMB2662.00

PKAα/β/γ cat (B-4): m-IgG2b BP-HRP 套装

sc-550106
200 µg Ab; 10 µg BP
RMB2662.00

PKAα/β/γ cat 抗体 (B-4) AC

sc-365615 AC
500 µg/ml, 25% agarose
RMB3129.00

PKAα/β/γ cat 抗体 (B-4) HRP

sc-365615 HRP
200 µg/ml
RMB2377.00

PKAα/β/γ cat 抗体 (B-4) FITC

sc-365615 FITC
200 µg/ml
RMB2482.00

PKAα/β/γ cat 抗体 (B-4) PE

sc-365615 PE
200 µg/ml
RMB2580.00

PKAα/β/γ cat 抗体 (B-4) Alexa Fluor® 488

sc-365615 AF488
200 µg/ml
RMB2685.00

PKAα/β/γ cat 抗体 (B-4) Alexa Fluor® 546

sc-365615 AF546
200 µg/ml
RMB2685.00

PKAα/β/γ cat 抗体 (B-4) Alexa Fluor® 594

sc-365615 AF594
200 µg/ml
RMB2685.00

PKAα/β/γ cat 抗体 (B-4) Alexa Fluor® 647

sc-365615 AF647
200 µg/ml
RMB2685.00

PKAα/β/γ cat 抗体 (B-4) Alexa Fluor® 680

sc-365615 AF680
200 µg/ml
RMB2685.00

PKAα/β/γ cat 抗体 (B-4) Alexa Fluor® 790

sc-365615 AF790
200 µg/ml
RMB2685.00

Can PKAα/β/γ cat (B-4): sc-365615 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PKAα/β/γ cat (B-4): sc-365615 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 功能很出色我先前使用了试用装,功能很优秀,条带清晰,实验次数之间不存在 误差,实验可顺利完成,由于实验需要 ,希望可再申请再试试
Date published: 2017-10-19
Rated 5 out of 5 by from ExcellentThis product works perfectly and I have to admit that it is so far the best antibody I have ever used
Date published: 2017-07-17
Rated 5 out of 5 by from great featuresI bought PKAα/β/γ cat antibody on November 2, 2016 , I am so happy that I chose this product ,we use this antibody to detect PKAα/β/γ catalytic subunit of mouse’s RAW264.7 cell line by WB. This product works perfectly and I have to admit that it is so far the best antibody I have ever used
Date published: 2016-12-06
Rated 5 out of 5 by from Positive signal in WB in MCF7 and PCPositive signal in WB in MCF7 and PC-3 whole cell lysates. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Good specificity in WB in MCF7 and PCGood specificity in WB in MCF7 and PC-3 whole cell lysates. -SCBT QC
Date published: 2015-03-28
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-20
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PKAα/β/γ cat 抗体 (B-4) is rated 5.0 out of 5 by 6.
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